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学科主题基础医学
Two-dimensional crystallization of the ryanodine receptor Ca2+ release channel on lipid membranes
Yin, CC1; Han, HM1; Wei, RS1; Lai, FA1
关键词Ca2++ Release Channel Electron Crystallography Ryanodine Receptor Two-dimensional Crystallization
刊名JOURNAL OF STRUCTURAL BIOLOGY
2005-02-01
DOI10.1016/j.jsb.2004.10.008
149期:2页:219-224
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Biophysics ; Cell Biology
研究领域[WOS]Biochemistry & Molecular Biology ; Biophysics ; Cell Biology
关键词[WOS]SKELETAL-MUSCLE ; SARCOPLASMIC-RETICULUM ; ELECTRON CRYOMICROSCOPY ; RECONSTITUTION ; JUNCTION ; PROTEIN ; SPARKS ; CELLS ; MODEL
英文摘要

The ryanodine receptor (RyR) is the largest known membrane protein with a total molecular mass of 2.3 x 10(3) kDa. Well ordered, two-dimensional (213) crystals are an essential prerequisite to enable RyR structure determination by electron crystallography. Conventionally, the 2D crystallization of membrane proteins is based on a ′trial-and-error′ strategy, which is both time-consuming and chance-directed. By adopting a new strategy that utilizes protein sequence information and predicted transmembrane topology, we successfully crystallized the RyR on positively charged lipid membranes. Image processing of negatively stained crystals reveals that they are well ordered, with diffraction spots of IQ less than or equal to 4 extending to similar to20 Angstrom, the resolution attainable in negative stain. The RyR crystals obtained on the charged lipid membrane have characteristics consistent with 2D arrays that have been observed in native sarcoplasmic reticulum of muscle tissues. These crystals provide ideal materials to enable structural analysis of RyR by high-resolution electron crystallography. Moreover, the reconstituted native-like 2D array provides an ideal model system to gain structural insights into the mechanism of RyR-mediated Ca2+ signaling processes, in which the intrinsic ability of RyR oligomers to organize into a 2D array plays a crucial role. (C) 2004 Elsevier Inc. All rights reserved.

语种英语
WOS记录号WOS:000226843800011
引用统计
被引频次:31[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/52754
专题北京大学基础医学院_生物物理学系
北京大学基础医学院
作者单位1.Peking Univ, Ctr Hlth Sci, Dept Biophys, Beijing 100083, Peoples R China
2.Wales Coll Med, Wales Heart Res Inst, Dept Cardiol, Cardiff CF14 4XN, S Glam, Wales
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Yin, CC,Han, HM,Wei, RS,et al. Two-dimensional crystallization of the ryanodine receptor Ca2+ release channel on lipid membranes[J]. JOURNAL OF STRUCTURAL BIOLOGY,2005,149(2):219-224.
APA Yin, CC,Han, HM,Wei, RS,&Lai, FA.(2005).Two-dimensional crystallization of the ryanodine receptor Ca2+ release channel on lipid membranes.JOURNAL OF STRUCTURAL BIOLOGY,149(2),219-224.
MLA Yin, CC,et al."Two-dimensional crystallization of the ryanodine receptor Ca2+ release channel on lipid membranes".JOURNAL OF STRUCTURAL BIOLOGY 149.2(2005):219-224.
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