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学科主题: 基础医学
题名:
Two-dimensional crystallization of the ryanodine receptor Ca2+ release channel on lipid membranes
作者: Yin, CC1; Han, HM1; Wei, RS1; Lai, FA1
关键词: Ca2+ release channel ; electron crystallography ; ryanodine receptor ; two-dimensional crystallization
刊名: JOURNAL OF STRUCTURAL BIOLOGY
发表日期: 2005-02-01
DOI: 10.1016/j.jsb.2004.10.008
卷: 149, 期:2, 页:219-224
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Biophysics ; Cell Biology
研究领域[WOS]: Biochemistry & Molecular Biology ; Biophysics ; Cell Biology
关键词[WOS]: SKELETAL-MUSCLE ; SARCOPLASMIC-RETICULUM ; ELECTRON CRYOMICROSCOPY ; RECONSTITUTION ; JUNCTION ; PROTEIN ; SPARKS ; CELLS ; MODEL
英文摘要:

The ryanodine receptor (RyR) is the largest known membrane protein with a total molecular mass of 2.3 x 10(3) kDa. Well ordered, two-dimensional (213) crystals are an essential prerequisite to enable RyR structure determination by electron crystallography. Conventionally, the 2D crystallization of membrane proteins is based on a ′trial-and-error′ strategy, which is both time-consuming and chance-directed. By adopting a new strategy that utilizes protein sequence information and predicted transmembrane topology, we successfully crystallized the RyR on positively charged lipid membranes. Image processing of negatively stained crystals reveals that they are well ordered, with diffraction spots of IQ less than or equal to 4 extending to similar to20 Angstrom, the resolution attainable in negative stain. The RyR crystals obtained on the charged lipid membrane have characteristics consistent with 2D arrays that have been observed in native sarcoplasmic reticulum of muscle tissues. These crystals provide ideal materials to enable structural analysis of RyR by high-resolution electron crystallography. Moreover, the reconstituted native-like 2D array provides an ideal model system to gain structural insights into the mechanism of RyR-mediated Ca2+ signaling processes, in which the intrinsic ability of RyR oligomers to organize into a 2D array plays a crucial role. (C) 2004 Elsevier Inc. All rights reserved.

语种: 英语
WOS记录号: WOS:000226843800011
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/52754
Appears in Collections:基础医学院_生物物理学系_期刊论文

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作者单位: 1.Peking Univ, Ctr Hlth Sci, Dept Biophys, Beijing 100083, Peoples R China
2.Wales Coll Med, Wales Heart Res Inst, Dept Cardiol, Cardiff CF14 4XN, S Glam, Wales

Recommended Citation:
Yin, CC,Han, HM,Wei, RS,et al. Two-dimensional crystallization of the ryanodine receptor Ca2+ release channel on lipid membranes[J]. JOURNAL OF STRUCTURAL BIOLOGY,2005,149(2):219-224.
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