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学科主题临床医学
Antitumor effects of oncolytic adenovirus armed with PSA-IZ-CD40L fusion gene against prostate cancer
Yang, Y-F1; Xue, S-Y1; Lu, Z-Z2; Xiao, F-J1; Yin, Y.3; Zhang, Q-W1; Wu, C-T1; Wang, H.1; Wang, L-S1
刊名GENE THERAPY
2014-08-01
DOI10.1038/gt.2014.46
21期:8页:723-731
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Genetics & Heredity ; Medicine, Research & Experimental
研究领域[WOS]Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Genetics & Heredity ; Research & Experimental Medicine
关键词[WOS]DENDRITIC CELLS ; IMMUNE-SYSTEM ; IMMUNOTHERAPY ; THERAPY ; APOPTOSIS ; PSA
英文摘要

Advanced prostate cancer (PC) still remains incurable. Novel immunogene therapy shows promise as treatment strategy that can target both localized and metastasized PC. In this study, we have developed a PC-specific oncolytic adenovirus (Ad-PL-PPT-E1A) armed with fusion gene of prostate-specific antigen and CD40 ligand, and aimed to evaluate its therapeutic effect in vitro and in vivo. After they were rescued in human embryonic kidney 293 cells, we confirmed that Ad-PL-PPT-ElA could mediate the expression of El A efficiently and produce abundant progeny viruses in PC cells in vitro. Our data showed that Ad-PL-PPT-ElA induced apoptosis and resulted in specific oncolytic toxicity in PC cells, which was detected by Annexin-V staining and crystal violet, respectively. After stimulation with lysates, immune phenotypes and cytokines expression of human dendritic cells was detected by flow cytometry and real-time polymerase chain reaction, respectively. And, the results showed that the lysate of Ad-PL-PPT-ElA infected LNCaP cells upregulated the expression of CD80, CD83, CD86 and mRNA level of interleukin-6 (IL-6), IL-12,1L-23 and tumor necrosis factor-a significantly. In established PC3M cell-xenografted mouse models, Ad-PL-PPT-ElA treatment improved the survival and suppressed the tumor growth obviously. In conclusion, Ad-PL-PPT-ElA exhibited enhanced antitumor activity is a promising approach for gene therapy of advanced PC.

语种英语
WOS记录号WOS:000340351900002
项目编号2012AA020807 ; 2014AA020515 ; 2012CB518205 ; 30901379
资助机构National High Technology Research and Development Program of China (863 Program) ; National Basic Research and Development of China (973 Program) ; National Natural Science Foundation of China
引用统计
被引频次:11[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/52759
专题北京大学第一临床医学院_血液内科
北京大学口腔医学院_人事处
作者单位1.Beijing Inst Radiat Med, Dept Expt Hematol, Beijing 100850, Peoples R China
2.Chinese Ctr Dis Control & Prevent, Inst Viral Dis Control & Prevent, Beijing, Peoples R China
3.Peking Univ, Hosp 1, Dept Hematol, Beijing 100871, Peoples R China
推荐引用方式
GB/T 7714
Yang, Y-F,Xue, S-Y,Lu, Z-Z,et al. Antitumor effects of oncolytic adenovirus armed with PSA-IZ-CD40L fusion gene against prostate cancer[J]. GENE THERAPY,2014,21(8):723-731.
APA Yang, Y-F.,Xue, S-Y.,Lu, Z-Z.,Xiao, F-J.,Yin, Y..,...&Wang, L-S.(2014).Antitumor effects of oncolytic adenovirus armed with PSA-IZ-CD40L fusion gene against prostate cancer.GENE THERAPY,21(8),723-731.
MLA Yang, Y-F,et al."Antitumor effects of oncolytic adenovirus armed with PSA-IZ-CD40L fusion gene against prostate cancer".GENE THERAPY 21.8(2014):723-731.
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