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学科主题基础医学
Cloning of crucian carp (Carassius cuvieri) metallothionein-II gene and characterization of its gene promoter region
Ren, HW1; Xu, MX1; He, PF1; Muto, N1; Itoh, N1; Tanaka, K1; Xing, J1; Chu, MM1
关键词metallothionein gene regulation MRE GRE Carassius cuvieri
刊名BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
2006-04-21
DOI10.1016/j.bbrc.2006.02.082
342期:4页:1297-1304
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]METAL-RESPONSIVE ELEMENTS ; REGULATORY ELEMENTS ; B-GENE ; FUNCTIONAL-ANALYSIS ; ZINC-METABOLISM ; CDNA CLONING ; COMMON CARP ; A GENE ; EXPRESSION ; FISH
英文摘要

The genomic DNA of crucian carp (Carassius cuvieri) metallothiownein-II (ccMT-II), with its upstream region was obtained. The sequence analysis Of its upstream region revealed several putative cis-acting elements including seven metal regulatory elements (MREs), three activator protein 1 (AP1), two glucocorticoid response element (GREs) etc. The seven MREs locate into two clusters, a distal cluster with four MREs within -800/-600 bp from the translation start site and a proximal cluster with three MREs close to TATA box. In transient luciferase gene expression assays, both of the distal and proximal cluster MREs have significantly shown synergistic effects in the transcription of ccMT-II gene; the proximal cluster of MREs serves as the major elements in metal inducing activity; Zn2+ and Cd2+ served as much stronger inducers than Cu2+ shown in ccMT-II expression. The two GRE homologous sequences in ccMT-II promoter showed not inductive in either HepG2 or HEK293. (c) 2006 Elsevier Inc. All rights reserved.

语种英语
WOS记录号WOS:000236316100043
Citation statistics
Cited Times:15[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/52770
Collection北京大学基础医学院_北京大学人类疾病基因研究中心
作者单位1.Peking Univ, Dept Biochem & Mol Biol, Coll Life Sci, Beijing 100871, Peoples R China
2.Peking Univ, Ctr Human Dis Genom, Beijing 100083, Peoples R China
3.Osaka Univ, Grad Sch Pharmaceut Sci, Dept Toxicol, Suita, Osaka 5650871, Japan
Recommended Citation
GB/T 7714
Ren, HW,Xu, MX,He, PF,et al. Cloning of crucian carp (Carassius cuvieri) metallothionein-II gene and characterization of its gene promoter region[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2006,342(4):1297-1304.
APA Ren, HW.,Xu, MX.,He, PF.,Muto, N.,Itoh, N.,...&Chu, MM.(2006).Cloning of crucian carp (Carassius cuvieri) metallothionein-II gene and characterization of its gene promoter region.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,342(4),1297-1304.
MLA Ren, HW,et al."Cloning of crucian carp (Carassius cuvieri) metallothionein-II gene and characterization of its gene promoter region".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 342.4(2006):1297-1304.
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