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学科主题: 基础医学
题名:
Cloning of crucian carp (Carassius cuvieri) metallothionein-II gene and characterization of its gene promoter region
作者: Ren, HW1; Xu, MX1; He, PF1; Muto, N1; Itoh, N1; Tanaka, K1; Xing, J1; Chu, MM1
关键词: metallothionein ; gene regulation ; MRE ; GRE ; Carassius cuvieri
刊名: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
发表日期: 2006-04-21
DOI: 10.1016/j.bbrc.2006.02.082
卷: 342, 期:4, 页:1297-1304
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]: Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]: METAL-RESPONSIVE ELEMENTS ; REGULATORY ELEMENTS ; B-GENE ; FUNCTIONAL-ANALYSIS ; ZINC-METABOLISM ; CDNA CLONING ; COMMON CARP ; A GENE ; EXPRESSION ; FISH
英文摘要:

The genomic DNA of crucian carp (Carassius cuvieri) metallothiownein-II (ccMT-II), with its upstream region was obtained. The sequence analysis Of its upstream region revealed several putative cis-acting elements including seven metal regulatory elements (MREs), three activator protein 1 (AP1), two glucocorticoid response element (GREs) etc. The seven MREs locate into two clusters, a distal cluster with four MREs within -800/-600 bp from the translation start site and a proximal cluster with three MREs close to TATA box. In transient luciferase gene expression assays, both of the distal and proximal cluster MREs have significantly shown synergistic effects in the transcription of ccMT-II gene; the proximal cluster of MREs serves as the major elements in metal inducing activity; Zn2+ and Cd2+ served as much stronger inducers than Cu2+ shown in ccMT-II expression. The two GRE homologous sequences in ccMT-II promoter showed not inductive in either HepG2 or HEK293. (c) 2006 Elsevier Inc. All rights reserved.

语种: 英语
WOS记录号: WOS:000236316100043
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/52770
Appears in Collections:基础医学院_北京大学人类疾病基因研究中心_期刊论文

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作者单位: 1.Peking Univ, Dept Biochem & Mol Biol, Coll Life Sci, Beijing 100871, Peoples R China
2.Peking Univ, Ctr Human Dis Genom, Beijing 100083, Peoples R China
3.Osaka Univ, Grad Sch Pharmaceut Sci, Dept Toxicol, Suita, Osaka 5650871, Japan

Recommended Citation:
Ren, HW,Xu, MX,He, PF,et al. Cloning of crucian carp (Carassius cuvieri) metallothionein-II gene and characterization of its gene promoter region[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2006,342(4):1297-1304.
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