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学科主题临床医学
Proliferation and anti-apoptotic effects of human urotensin II on human endothelial cells
Shi, Libin()1; Ding, Wenghui()1; Li, Dayuan()1; Wang, Zhijian()1; Jiang, Hongfeng()1; Zhang, Junhua()1; Tang, Chaoshu()1
关键词Human Urotensin Ii Proliferation Apoptosis Protein Kinases Endothelial Cells
刊名ATHEROSCLEROSIS
2006-10-01
DOI10.1016/j.atherosclerosis.2005.10.044
188期:2页:260-264
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Peripheral Vascular Disease
研究领域[WOS]Cardiovascular System & Cardiology
关键词[WOS]CONGESTIVE-HEART-FAILURE ; SMOOTH-MUSCLE-CELLS ; RECEPTOR GPR14 ; MAST-CELLS ; IN-VIVO ; VASOCONSTRICTOR ; EXPRESSION ; DENSITY ; LDL
英文摘要

Background: Human urotensin II (hU-II) is a potent vasoconstrictor, highly expressed in cardiac tissues and blood vessels. Recent studies indicate that hU-II participates in vascular and myocardial remodeling after injury. This study was designed to study the role of hU-II in cell DNA synthesis and apoptosis in human umbilical vein endothelial cells (HUVECs) and underlying intracellular signaling mechanisms.

Methods and Results: Cultured HUVECs were incubated with hU-II (10(-10)-10(-8) M) for 24 h. Cell DNA synthesis was examined by 3H thymidine incorporation. Apoptosis was detected by flow cytometry and TUNEL. hU-II increased the 3H thymidine incorporation into DNA in a concentration-dependent manner. hU-II inhibited endothelial apoptosis induced by serum withdrawal (5.74 +/- 0.64% versus 13.20 +/- 1.96%, P < 0.01) and TNF alpha (6.76 +/- 0.70% versus 13.80 +/- 1.02%, P < 0.01). The data from flow cytometry and TUNEL are consistent. Further studies showed that hU-II caused the phosphorylation of mitogen-activated protein kinasep42/44 (MAPKp42/44) in a concentration-dependent manner and this effect of hU-II was inhibited by pretreatment of cells with the MEK inhibitor (PD98059, 10 mu M). In addition, the use of PD98059 also attenuated 3H thymidine incorporation and anti-apoptotic effect elicited by hU-II (both P < 0.01 versus hU-II alone).

Conclusions: Our observations provide evidence that hU-II promotes cell proliferation and inhibits apoptosis in HUVECs, and MAPKp42/44 activation may play a signal transduction role in this process. (c) 2005 Elsevier Ireland Ltd. All rights reserved.

语种英语
WOS记录号WOS:000240933300005
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被引频次:43[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/52782
专题北京大学第一临床医学院_心血管内科
作者单位1.Peking Univ, Hosp 1, Dept Internal Med, Div Cardiol, Beijing 100034, Peoples R China
2.Univ Arkansas Med Sci, Div Cardiovasc Med, Little Rock, AR 72205 USA
3.Peking Univ, Dept Physiol, Beijing 100083, Peoples R China
推荐引用方式
GB/T 7714
Shi, Libin,Ding, Wenghui,Li, Dayuan,et al. Proliferation and anti-apoptotic effects of human urotensin II on human endothelial cells[J]. ATHEROSCLEROSIS,2006,188(2):260-264.
APA Shi, Libin.,Ding, Wenghui.,Li, Dayuan.,Wang, Zhijian.,Jiang, Hongfeng.,...&Tang, Chaoshu.(2006).Proliferation and anti-apoptotic effects of human urotensin II on human endothelial cells.ATHEROSCLEROSIS,188(2),260-264.
MLA Shi, Libin,et al."Proliferation and anti-apoptotic effects of human urotensin II on human endothelial cells".ATHEROSCLEROSIS 188.2(2006):260-264.
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