学科主题 | 临床医学 |
L-cystathionine inhibits oxidized low density lipoprotein-induced THP-1-derived macrophage inflammatory cytokine monocyte chemoattractant protein-1 generation via the NF-kappa B pathway | |
Zhu, Mingzhu1; Du, Junbao1,2,3; Liu, Angie Dong4; Holmberg, Lukas4; Chen, Selena Y.5; Bu, Dingfang6; Tang, Chaoshu2,7; Jin, Hongfang1 | |
刊名 | SCIENTIFIC REPORTS
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2015-05-28 | |
DOI | 10.1038/srep10453 |
卷 | 5 |
收录类别 | SCI |
文章类型 | Article |
WOS标题词 | Science & Technology |
类目[WOS] | Multidisciplinary Sciences |
资助者 | National Natural Science Foundation of China ; Major Basic Research Project of China ; Program for New Century Excellent Talents of Ministry of Education, China ; National Natural Science Foundation of China ; Major Basic Research Project of China ; Program for New Century Excellent Talents of Ministry of Education, China |
研究领域[WOS] | Science & Technology - Other Topics |
关键词[WOS] | HYDROGEN-SULFIDE ; ATHEROSCLEROSIS ; CELLS ; EXPRESSION ; GENE ; NEUTROPHILS ; METABOLISM ; APOPTOSIS ; PROTECTS ; KETIMINE |
英文摘要 | This study aimed to explore whether and how L-cystathionine had any regulatory effect on the inflammatory response in THP-1-derived macrophages cultured in vitro under oxidized low-density lipoprotein (ox-LDL) stimulation. The human monocyte line THP-1 cell was cultured in vitro and differentiated into macrophages after 24 hours of PMA induction. Macrophages were pretreated with L-cystathionine and then treated with ox-LDL. The results showed that compared with the controls, ox-LDL stimulation significantly upregulated the expression of THP-1-derived macrophage MCP-1 by enhancing NF-kappa B p65 phosphorylation, nuclear translocation and DNA binding with the MCP-1 promoter. Compared with the ox-LDL group, 0.3 mmol/L and 1.0 mmol/L L-cystathionine significantly inhibited the expression of THP-1-derived macrophage MCP-1. Mechanistically, 0.3 mmol/L and 1.0 mmol/L L-cystathionine suppressed phosphorylation and nuclear translocation of the NF-kappa B p65 protein, as well as the DNA binding activity and DNA binding level of NF-kappa B with the MCP-1 promoter, which resulted in a reduced THP-1-derived macrophage MCP-1 generation. This study suggests that L-cystathionine could inhibit the expression of MCP-1 in THP-1-derived macrophages induced by ox-LDL via inhibition of NF-kappa B p65 phosphorylation, nuclear translocation, and binding of the MCP-1 promoter sequence after entry into the nucleus. |
语种 | 英语 |
所属项目编号 | 31130030 ; 81370154 ; 91439110 ; 2012CB517806 ; 2011CB503904 ; NCET-11-0005 |
资助者 | National Natural Science Foundation of China ; Major Basic Research Project of China ; Program for New Century Excellent Talents of Ministry of Education, China ; National Natural Science Foundation of China ; Major Basic Research Project of China ; Program for New Century Excellent Talents of Ministry of Education, China |
WOS记录号 | WOS:000355546600001 |
Citation statistics | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.bjmu.edu.cn/handle/400002259/52783 |
Collection | 北京大学第一临床医学院_儿科 |
作者单位 | 1.Peking Univ, Hosp 1, Cent Lab, Beijing 100034, Peoples R China 2.Peking Univ, Hosp 1, Dept Pediat, Beijing 100034, Peoples R China 3.Minist Educ, Key Lab Mol Cardiol, Beijing 100191, Peoples R China 4.Linkoping Univ, Dept Med & Hlth Sci, S-58183 Linkoping, Sweden 5.Univ Calif San Diego, La Jolla, CA 92093 USA 6.Peking Univ, Hlth Sci Ctr, Ctr Mol & Translat Med, Beijing 100191, Peoples R China 7.Peking Univ, Hlth Sci Ctr, Dept Physiol & Pathophysiol, Beijing 100191, Peoples R China |
Recommended Citation GB/T 7714 | Zhu, Mingzhu,Du, Junbao,Liu, Angie Dong,et al. L-cystathionine inhibits oxidized low density lipoprotein-induced THP-1-derived macrophage inflammatory cytokine monocyte chemoattractant protein-1 generation via the NF-kappa B pathway[J]. SCIENTIFIC REPORTS,2015,5. |
APA | Zhu, Mingzhu.,Du, Junbao.,Liu, Angie Dong.,Holmberg, Lukas.,Chen, Selena Y..,...&Jin, Hongfang.(2015).L-cystathionine inhibits oxidized low density lipoprotein-induced THP-1-derived macrophage inflammatory cytokine monocyte chemoattractant protein-1 generation via the NF-kappa B pathway.SCIENTIFIC REPORTS,5. |
MLA | Zhu, Mingzhu,et al."L-cystathionine inhibits oxidized low density lipoprotein-induced THP-1-derived macrophage inflammatory cytokine monocyte chemoattractant protein-1 generation via the NF-kappa B pathway".SCIENTIFIC REPORTS 5(2015). |
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