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学科主题: 基础医学
题名:
Study on method of determining CD4 on T lymphocyte.
作者: Han, M1; Lu, P1; He, QH1; Yuan, L1; Ma, SL1; Tiao, JP1
关键词: T lymphocyte ; CD4 ; flow cytometry ; laser scanning confocal microscopy
刊名: PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS
发表日期: 2000-04-01
卷: 27, 期:2, 页:218-220
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]: Biochemistry & Molecular Biology ; Biophysics
英文摘要:

To investigate the differentiation of pre-preparation method to determine CD4 on T lymphocyte in blood, CD4 expression on T lymphocyte of monkey was determined by the flow cytometry as a quantitative analysis with three different pre-preparation methods. These results showed that (b) method (whole blood was labeled by FITC-CD4 McAb. Then red blood cells were dissolved by erythrocytolysis) was better (a) method (red blood cells were dissolved by erythrocytolysis in whole blood. Then it was labeled by FITC-CD4 McAb). CD4 results with (b) method were equal to (c) method ( traditional method of lymphocyte separated). But the volume of sample by (b) method only was 1/5 of (c) method. The pre-preparation of (b) method also was sampler than (c) method. Morphologic study by laser scanning confocal microscopy showed (b) method was similar to (c) method. Both of them had a clear fluorescent labeled on the cell membrane but not in the cell.

语种: 中文
WOS记录号: WOS:000086656900029
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/52788
Appears in Collections:基础医学院_免疫学系_期刊论文

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作者单位: 1.Beijing Med Univ, Med & Hlth Analyt Ctr, Beijing 100083, Peoples R China
2.Beijing Med Univ, Dept Immunol, Beijing 100083, Peoples R China

Recommended Citation:
Han, M,Lu, P,He, QH,et al. Study on method of determining CD4 on T lymphocyte.[J]. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,2000,27(2):218-220.
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