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学科主题: 临床医学
题名:
SARS-CoV nucleocapsid protein induced apoptosis of COS-1 mediated by the mitochondrial pathway
作者: Zhang, Lu; Wei, Lai; Jiang, Dong; Wang, Jianghua; Cong, Xu; Fei, Ran
关键词: apoptosis ; mitochondrial pathway ; reactive oxygen species (ROS) ; SARS coronavirus nuleocapsid
刊名: ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY
发表日期: 2007
DOI: 10.1080/10731190601188422
卷: 35, 期:2, 页:237-253
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biotechnology & Applied Microbiology ; Engineering, Biomedical ; Materials Science, Biomaterials
研究领域[WOS]: Biotechnology & Applied Microbiology ; Engineering ; Materials Science
关键词[WOS]: ACUTE RESPIRATORY SYNDROME ; CORONAVIRUS ; CELLS ; PHOSPHATIDYLSERINE ; ACTIVATION ; INDUCTION ; CASPASE-3 ; PATHOLOGY ; DOMAIN
英文摘要:

To investigate the apoptosis effect of SARS coronavirus nucleocapsid protein on cultured cell lines and to explore the possible pathway of apoptosis. pCDNA3.1(-)/his-myc vector containing the SARS coronavirus nucleocapsid gene (N), matric gene (M), spike gene (S) were transfected into COS-1, Huh-7 and HepG2 cells. Apoptosis induced by SARS coronavirus N protein under starvation of serum of COS-1 cells was monitored by Annexin V and electron microscopy assays. Intracellular reactive oxygen species (ROS) and mitochondrial membrane potential (Delta Psi m) were determined by flow cytometric assay. Cytochrome C, cleaved caspase (cysteine aspartic acid protease)-3, 9, and poly (ADP-ribose) polymerase (PARP) were detected by Western blot. After removal of serum in COS-1 cells, we observed the loss of Delta Psi m, the increase of ROS and cytochrome C release into cytosol and subsequent activation of caspase-3 and PARP cleavage. The pan-caspase inhibitor z-VAD-fmk can block the activation of caspase 3, 9 and PARP cleavage. In conclusion, SARS coronavirus N protein can induce apoptosis of COS-1 cells by activating mitochondrial pathway. SARS coronavirus M, S protein can not induce apoptosis in COS-1, HepG2 and Huh-7 and SARS coronavirus N protein can not induce apoptosis in HepG2 and Huh-7 by methods used in this study.

语种: 英语
WOS记录号: WOS:000245791700007
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/52816
Appears in Collections:北京大学第二临床医学院_北京大学肝病研究所_期刊论文

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作者单位: Peking Univ, Hlth Sci Ctr, Peoples Hosp, Inst Hepatol, Beijing 100044, Peoples R China

Recommended Citation:
Zhang, Lu,Wei, Lai,Jiang, Dong,et al. SARS-CoV nucleocapsid protein induced apoptosis of COS-1 mediated by the mitochondrial pathway[J]. ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY,2007,35(2):237-253.
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