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学科主题基础医学
Involvement of STAT5a signaling in morphine-induced up-regulation of the cyclin D1
Guo, Liyuan1; Li, Hui1; Liu, Han1; Li, Chaoying1; Li, Mengsen3; Jiang, Wei1; He, Peng1; Wang, Shanshan1; McNutt, Michael A.2; Li, Gang1
关键词Morphine Cyclin D1 STAT5a Signaling Lymphocytes
刊名BIOCHEMICAL PHARMACOLOGY
2009-05-01
DOI10.1016/j.bcp.2009.02.007
77期:9页:1553-1560
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Pharmacology & Pharmacy
研究领域[WOS]Pharmacology & Pharmacy
关键词[WOS]MU-OPIOID RECEPTOR ; SIMIAN IMMUNODEFICIENCY VIRUS ; CEM X174 CELLS ; G-PROTEIN ; IN-VITRO ; ACTIVATION ; EXPRESSION ; APOPTOSIS ; TRANSCRIPTION ; SURVIVAL
英文摘要

Opioid receptors and cytokine receptor have been verified to have a functional link and interaction. However, the pathway by which opioid receptor in lymphocytes is linked to cytokine signaling is not well defined. Using confocal microscopy and Western blotting this study showed that morphine treatment was able to activate cytoplasmic STAT5a in CEM x 174 cells, which then translocated into the nucleus and bound to elements of the cyclin D1 promoter. As a consequence the expression of the cyclin D1 was apparently up-regulated. The data from EMSA-superEMSA and ChlP-qPCR further confirmed that morphine was capable of promoting the binding of STAT5a to its elements (proximal and distal), and this was abolished by the antagonist naloxone. As shown by transient transfection assay, activity of the cyclin D1 promoter was significantly reduced by 82% (distal) and 65% (proximal) after two STAT5a elements were mutated in comparison with wild type STAT5a elements. Moreover, knockdown of STAT5a was associated with a concurrent silencing of morphine-induced expression of cyclin D1. demonstrating involvement of STAT5a in morphine-triggered signaling in the regulation of cyclin D1 expression. The finding provides evidence which demonstrates that there is cross-talk between the mu opioid receptor and cytokine signaling in lymphocytes, Thus, we conclude that morphine may modulate cyclin D1 gene expression via signal transducers and activators of transcription (STATs) signaling, which will be beneficial for further understanding of the pharmacological effect of morphine on immune regulation. (C) 2009 Elsevier Inc. All rights reserved.

语种英语
WOS记录号WOS:000265197000012
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被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/52826
专题北京大学基础医学院_病理学系
作者单位1.Hainan Med Coll, Key Lab Mol Biol, Haikou 571101, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Dept Biochem & Mol Biol, Beijing 100083, Peoples R China
3.Peking Univ, Hlth Sci Ctr, Dept Pathol, Beijing 100083, Peoples R China
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GB/T 7714
Guo, Liyuan,Li, Hui,Liu, Han,et al. Involvement of STAT5a signaling in morphine-induced up-regulation of the cyclin D1[J]. BIOCHEMICAL PHARMACOLOGY,2009,77(9):1553-1560.
APA Guo, Liyuan.,Li, Hui.,Liu, Han.,Li, Chaoying.,Li, Mengsen.,...&Li, Gang.(2009).Involvement of STAT5a signaling in morphine-induced up-regulation of the cyclin D1.BIOCHEMICAL PHARMACOLOGY,77(9),1553-1560.
MLA Guo, Liyuan,et al."Involvement of STAT5a signaling in morphine-induced up-regulation of the cyclin D1".BIOCHEMICAL PHARMACOLOGY 77.9(2009):1553-1560.
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