北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学第二临床医学院  > 血液科  > 期刊论文
学科主题: 临床医学
题名:
Significance of cytogenetic and fluorescence in situ hybridization analysis in evaluating antichronic myeloid leukemia efficacy of different immune effector cells
作者: Tong, CR1; Hong, B1; Qiu, JY1; Chen, Z1; Lu, DP1
刊名: CANCER GENETICS AND CYTOGENETICS
发表日期: 2002-04-01
卷: 134, 期:1, 页:21-24
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology ; Genetics & Heredity
研究领域[WOS]: Oncology ; Genetics & Heredity
英文摘要:

We report on the antileukemia effect of interleukin 2 (IL2) on different immune cells from 22 patients with chronic myeloid leukemia (CML). Bone marrow cells from these patients were first cultured in modified long-term bone marrow culture medium for several days, then separately cultured with lymphokine activated killer cells (LAK), cytokine-induced killer cells (CIK), and dendritic cell cocultured CIK (DCCIK) for another 1-2 days. They were then detected for presence of the Philadelphia chromosome (Ph) by cytogenetic analysis and fluorescence in situ hybridization (FISH). The percentage of Ph-chromosome-positive cells in the bone marrow mononuclear cells after culturing with CIK and DC-CIK was significantly lower than that after culturing with IL2 or LAK. Our results demonstrate that cytogenetics and FISH are useful techniques for the evaluation of the anti-CML effect of immune cells and that CIK or DCCIK can be appropriate candidates for adoptive immune cell therapy in vivo or for leukemia cell purging ex vivo. (C) 2002 Elsevier Science Inc. All rights reserved.

语种: 英语
WOS记录号: WOS:000175165700005
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/52841
Appears in Collections:北京大学第二临床医学院_血液科_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Peking Univ, Inst Hematol, Cellular Therapy Ctr, Beijing 100044, Peoples R China
2.Peking Univ, Peoples Hosp, Inst Hematol, Genet & Cytogenet Lab, Beijing 100044, Peoples R China
3.Univ Utah, Sch Med, Div Med Genet, Salt Lake City, UT 84123 USA

Recommended Citation:
Tong, CR,Hong, B,Qiu, JY,et al. Significance of cytogenetic and fluorescence in situ hybridization analysis in evaluating antichronic myeloid leukemia efficacy of different immune effector cells[J]. CANCER GENETICS AND CYTOGENETICS,2002,134(1):21-24.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Tong, CR]'s Articles
[Hong, B]'s Articles
[Qiu, JY]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Tong, CR]‘s Articles
[Hong, B]‘s Articles
[Qiu, JY]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace