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Significance of cytogenetic and fluorescence in situ hybridization analysis in evaluating antichronic myeloid leukemia efficacy of different immune effector cells
Tong, CR1; Hong, B1; Qiu, JY1; Chen, Z1; Lu, DP1
刊名CANCER GENETICS AND CYTOGENETICS
2002-04-01
134期:1页:21-24
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology ; Genetics & Heredity
研究领域[WOS]Oncology ; Genetics & Heredity
英文摘要

We report on the antileukemia effect of interleukin 2 (IL2) on different immune cells from 22 patients with chronic myeloid leukemia (CML). Bone marrow cells from these patients were first cultured in modified long-term bone marrow culture medium for several days, then separately cultured with lymphokine activated killer cells (LAK), cytokine-induced killer cells (CIK), and dendritic cell cocultured CIK (DCCIK) for another 1-2 days. They were then detected for presence of the Philadelphia chromosome (Ph) by cytogenetic analysis and fluorescence in situ hybridization (FISH). The percentage of Ph-chromosome-positive cells in the bone marrow mononuclear cells after culturing with CIK and DC-CIK was significantly lower than that after culturing with IL2 or LAK. Our results demonstrate that cytogenetics and FISH are useful techniques for the evaluation of the anti-CML effect of immune cells and that CIK or DCCIK can be appropriate candidates for adoptive immune cell therapy in vivo or for leukemia cell purging ex vivo. (C) 2002 Elsevier Science Inc. All rights reserved.

语种英语
WOS记录号WOS:000175165700005
引用统计
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/52841
专题北京大学第二临床医学院_血液科
作者单位1.Peking Univ, Inst Hematol, Cellular Therapy Ctr, Beijing 100044, Peoples R China
2.Peking Univ, Peoples Hosp, Inst Hematol, Genet & Cytogenet Lab, Beijing 100044, Peoples R China
3.Univ Utah, Sch Med, Div Med Genet, Salt Lake City, UT 84123 USA
推荐引用方式
GB/T 7714
Tong, CR,Hong, B,Qiu, JY,et al. Significance of cytogenetic and fluorescence in situ hybridization analysis in evaluating antichronic myeloid leukemia efficacy of different immune effector cells[J]. CANCER GENETICS AND CYTOGENETICS,2002,134(1):21-24.
APA Tong, CR,Hong, B,Qiu, JY,Chen, Z,&Lu, DP.(2002).Significance of cytogenetic and fluorescence in situ hybridization analysis in evaluating antichronic myeloid leukemia efficacy of different immune effector cells.CANCER GENETICS AND CYTOGENETICS,134(1),21-24.
MLA Tong, CR,et al."Significance of cytogenetic and fluorescence in situ hybridization analysis in evaluating antichronic myeloid leukemia efficacy of different immune effector cells".CANCER GENETICS AND CYTOGENETICS 134.1(2002):21-24.
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