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Synthesis of Cibacron Blue F3GA-coupled magnetic PMMA nanospheres and their use for protein affinity separation
Qu, Feng2; Guan, Yueping1; Ma, Zhiya3; Zhang, Qiang2
关键词Magnetic Pmma Nanospheres Cibacron Blue F3ga Bovine Serum Albumin Affinity Separation Adsorption Capacity
刊名POLYMER INTERNATIONAL
2009-08-01
DOI10.1002/pi.2607
58期:8页:888-892
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Polymer Science
研究领域[WOS]Polymer Science
关键词[WOS]POLY(2-HYDROXYETHYL METHACRYLATE) BEADS ; ALBUMIN ADSORPTION ; MINIEMULSION POLYMERIZATION ; SUSPENSION POLYMERIZATION ; SURFACE MODIFICATION ; CHROMATOGRAPHY ; POLYSTYRENE ; MICROSPHERES ; PURIFICATION ; PARTICLES
英文摘要

BACKGROUND: New magnetic carrier separation technologies, capable of treating dilute solutions in large-scale processes, even in the presence of biological debris, are necessary for the future development of biotechnology. Non-porous magnetic carriers are more resistant to fouling, show better mass transfer and have lower non-specific adsorption than porous carriers. Nanosized magnetic carriers have a surface area comparable to that of typical macroporous resins, and therefore their application has advantages.

RESULTS: Magnetic poly(methyl methacrylate) (PMMA) nanospheres with an average diameter of 76 nm and narrow size distribution were prepared by a facile mini-emulsion polymerization. After surface modification with poly(ethylene glycol), Cibacron Blue F3GA (CB) was coupled to the magnetic PMMA nanospheres to form dye ligand-attached magnetic adsorbents for bovine serum albumin (BSA) adsorption. The CB-coupled magnetic PMMA nanospheres showed very high adsorption capacity (1121.98 mg g(-1)) and little non-specific adsorption for BSA. The adsorbed protein could be easily desorbed using high ionic strength solution.

CONCLUSION: The CB-coupled magnetic PMMA nanospheres showed a high BSA adsorption capacity, low non-specific adsorption and fast adsorption kinetics in comparison with other dye-affinity adsorbents. These characteristics indicate that these magnetic PMMA nanospheres have great potential for protein affinity separation and purification. (C) 2009 Society of Chemical Industry

语种英语
WOS记录号WOS:000268558600008
项目编号2007AA03Z315 ; 2073025
资助机构National High Technology Research and Development Program of China ; Beijing Natural Science Foundation of China
引用统计
被引频次:20[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/52842
专题北京大学药学院
北京大学药学院_药剂学系
作者单位1.Univ Sci & Technol Beijing, Sch Mat Sci & Engn, Beijing 100083, Peoples R China
2.Peking Univ, Sch Pharmaceut Sci, Beijing 100083, Peoples R China
3.Chinese Acad Sci, State Key Lab Biochem Engn, Inst Proc Engn, Beijing 100080, Peoples R China
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GB/T 7714
Qu, Feng,Guan, Yueping,Ma, Zhiya,et al. Synthesis of Cibacron Blue F3GA-coupled magnetic PMMA nanospheres and their use for protein affinity separation[J]. POLYMER INTERNATIONAL,2009,58(8):888-892.
APA Qu, Feng,Guan, Yueping,Ma, Zhiya,&Zhang, Qiang.(2009).Synthesis of Cibacron Blue F3GA-coupled magnetic PMMA nanospheres and their use for protein affinity separation.POLYMER INTERNATIONAL,58(8),888-892.
MLA Qu, Feng,et al."Synthesis of Cibacron Blue F3GA-coupled magnetic PMMA nanospheres and their use for protein affinity separation".POLYMER INTERNATIONAL 58.8(2009):888-892.
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