IR@PKUHSC  > 北京大学第二临床医学院
学科主题临床医学
Comparative genomics of methicillin-resistant Staphylococcus aureus ST239: distinct geographical variants in Beijing and Hong Kong
Wang, Zheng1; Zhou, Haokui1; Wang, Hui2; Chen, Hongbin2; Leung, K. K.3; Tsui, Stephen3; Ip, Margaret1
关键词Mrsa Beijing Hong Kong Genome Orthologous Gene Groups St239
刊名BMC GENOMICS
2014-06-26
DOI10.1186/1471-2164-15-529
15
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biotechnology & Applied Microbiology ; Genetics & Heredity
研究领域[WOS]Biotechnology & Applied Microbiology ; Genetics & Heredity
关键词[WOS]SEQUENCE TYPE 239 ; INTERCONTINENTAL SPREAD ; CLONE ; VIRULENCE ; CHINA ; MRSA ; EVOLUTION
英文摘要

Background: The ST239 lineage is a globally disseminated, multiply drug-resistant hospital-associated methicillin-resistant Staphylococcus aureus (HA-MRSA). We performed whole-genome sequencing of representative HA-MRSA isolates of the ST239 lineage from bacteremic patients in hospitals in Hong Kong (HK) and Beijing (BJ) and compared them with three published complete genomes of ST239, namely T0131, TW20 and JKD6008. Orthologous gene group (OGG) analyses of the Hong Kong and Beijing cluster strains were also undertaken.

Results: Homology analysis, based on highest-percentage nucleotide identity, indicated that HK isolates were closely related to TW20, whereas BJ isolates were more closely related to T0131 from Tianjin. Phylogenetic analysis, incorporating a total of 30 isolates from different continents, revealed that strains from HK clustered with TW20 into the ′Asian clade′, whereas BJ isolates and T0131 clustered closely with strains of the ′Turkish clade′ from Eastern Europe. HK isolates contained the typical phi SP beta-like prophage with the SasX gene similar to TW20. In contrast, BJ isolates contained a unique 15 kb PT1028-like prophage but lacked phi SP beta-like and phi SA1 prophages. Besides distinct mobile genetic elements (MGE) in the two clusters, OGG analyses and whole-genome alignment of these clusters highlighted differences in genes located in the core genome, including the identification of single nucleotide deletions in several genes, resulting in frameshift mutations and the subsequent predicted truncation of encoded proteins involved in metabolism and antimicrobial resistance.

Conclusions: Comparative genomics, based on de novo assembly and deep sequencing of HK and BJ strains, revealed different origins of the ST239 lineage in northern and southern China and identified differences between the two clades at single nucleotide polymorphism (SNP), core gene and MGE levels. The results suggest that ST239 strains isolated in Hong Kong since the 1990s belong to the Asian clade, present mainly in southern Asia, whereas those that emerged in northern China were of a distinct origin, reflecting the complexity of dissemination and the dynamic evolution of this ST239 lineage.

语种英语
WOS记录号WOS:000338252200001
项目编号CU-09-05-01
资助机构Research Fund for the Control of Infectious Diseases (Health and Food Bureau, HKSAR) ; Wellcome Trust
引用统计
被引频次:9[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/52845
专题北京大学第二临床医学院
作者单位1.Chinese Univ Hong Kong, Dept Microbiol, Prince Wales Hosp, Shatin, Hong Kong, Peoples R China
2.Peking Univ, Peoples Hosp, Dept Clin Lab, Beijing 100044, Peoples R China
3.Chinese Univ Hong Kong, Sch Biomed Sci, Shatin, Hong Kong, Peoples R China
推荐引用方式
GB/T 7714
Wang, Zheng,Zhou, Haokui,Wang, Hui,et al. Comparative genomics of methicillin-resistant Staphylococcus aureus ST239: distinct geographical variants in Beijing and Hong Kong[J]. BMC GENOMICS,2014,15.
APA Wang, Zheng.,Zhou, Haokui.,Wang, Hui.,Chen, Hongbin.,Leung, K. K..,...&Ip, Margaret.(2014).Comparative genomics of methicillin-resistant Staphylococcus aureus ST239: distinct geographical variants in Beijing and Hong Kong.BMC GENOMICS,15.
MLA Wang, Zheng,et al."Comparative genomics of methicillin-resistant Staphylococcus aureus ST239: distinct geographical variants in Beijing and Hong Kong".BMC GENOMICS 15(2014).
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