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学科主题临床医学
Rapid identification of Fusarium graminearum species complex using Rolling Circle Amplification (RCA)
Davari, Mahdi1,2; van Diepeningen, Anne D.1; Babai-Ahari, Assadollah2; Arzanlou, Mahdi2; Najafzadeh, Mohammed Javad3; van der Lee, Theo A. J.4; de Hoog, G. Sybren1,5,6,7
关键词Rca Identification Fusarium Head Blight Fusarium Graminearum Species Complex (Fgsc) Fusarium Incarnatum-equiseti Species Complex (Fiesc) Mycotoxins Gramineae
刊名JOURNAL OF MICROBIOLOGICAL METHODS
2012-04-01
DOI10.1016/j.mimet.2012.01.017
89期:1页:63-70
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemical Research Methods ; Microbiology
资助者Iranian Ministry of Science, Research and Technology, University of Tabriz ; Iranian Ministry of Science, Research and Technology, University of Tabriz
研究领域[WOS]Biochemistry & Molecular Biology ; Microbiology
关键词[WOS]HEAD BLIGHT PATHOGEN ; DNA-SEQUENCE DATABASE ; GENEALOGICAL CONCORDANCE ; PADLOCK PROBES ; NORTH-AMERICA ; WHEAT ; MYCOTOXINS ; CEREALS ; BARLEY ; POPULATIONS
英文摘要

Rolling Circle Amplification (RCA) of DNA is a sensitive and cost effective method for the rapid identification of pathogenic fungi without the need for sequencing. Amplification products can be visualized on 1% agarose gel to verify the specificity of probe-template binding or directly by adding fluorescent dyes. Fusarium Head Blight (FHB) is currently the world′s largest threat to the production of cereal crops with the production of a range of mycotoxins as an additional risk. We designed sets of RCA padlock probes based on polymorphisms in the elongation factor 1-alpha (EF-1 alpha) gene to detect the dominant FHB species, comprising lineages of the Fusarium graminearum species complex (FGSC). The method also enabled the identification of species of the Fusarium oxysporum (FOSC), the Fusarium incarnatum-equiseti (FIESC), and the Fusarium tricinctum (FTSC) species complexes, and used strains from the CBS culture collection as reference. Subsequently probes were applied to characterize isolates from wheat and wild grasses, and inoculated wheat kernels. The RCA assays successfully amplified DNA of the target fungi, both in environmental samples and in the contaminated wheat samples, while no cross reactivity was observed with uncontaminated what or related Fusarium species. As RCA does not require expensive instrumentation, the technique has a good potential for local and point of care screening for toxigenic Fusarium species in cereals. (C) 2012 Elsevier B.V. All rights reserved.

语种英语
资助者Iranian Ministry of Science, Research and Technology, University of Tabriz ; Iranian Ministry of Science, Research and Technology, University of Tabriz
WOS记录号WOS:000302760700012
Citation statistics
Cited Times:12[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/52853
Collection北京大学第一临床医学院_皮肤性病科
作者单位1.CBS KNAW Fungal Biodivers Ctr, NL-3584 CT Utrecht, Netherlands
2.Univ Tabriz, Dept Plant Pathol, Fac Agr, Tabriz, Iran
3.Mashhad Univ Med Sci, Dept Parasitol & Mycol, Ghaem Hosp, Sch Med, Mashhad, Iran
4.Plant Res Int, Biointeract & Plant Hlth, NL-6708 PB Wageningen, Netherlands
5.Univ Amsterdam, Inst Biodivers & Ecosyst Dynam, Amsterdam, Netherlands
6.Peking Univ, Hlth Sci Ctr, Res Ctr Med Mycol, Beijing 100871, Peoples R China
7.Sun Yat Sen Univ, Sun Yat Sen Mem Hosp, Guangzhou 510275, Guangdong, Peoples R China
Recommended Citation
GB/T 7714
Davari, Mahdi,van Diepeningen, Anne D.,Babai-Ahari, Assadollah,et al. Rapid identification of Fusarium graminearum species complex using Rolling Circle Amplification (RCA)[J]. JOURNAL OF MICROBIOLOGICAL METHODS,2012,89(1):63-70.
APA Davari, Mahdi.,van Diepeningen, Anne D..,Babai-Ahari, Assadollah.,Arzanlou, Mahdi.,Najafzadeh, Mohammed Javad.,...&de Hoog, G. Sybren.(2012).Rapid identification of Fusarium graminearum species complex using Rolling Circle Amplification (RCA).JOURNAL OF MICROBIOLOGICAL METHODS,89(1),63-70.
MLA Davari, Mahdi,et al."Rapid identification of Fusarium graminearum species complex using Rolling Circle Amplification (RCA)".JOURNAL OF MICROBIOLOGICAL METHODS 89.1(2012):63-70.
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