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学科主题: 临床医学
题名:
Rapid identification of Fusarium graminearum species complex using Rolling Circle Amplification (RCA)
作者: Davari, Mahdi1,2; van Diepeningen, Anne D.1; Babai-Ahari, Assadollah2; Arzanlou, Mahdi2; Najafzadeh, Mohammed Javad3; van der Lee, Theo A. J.4; de Hoog, G. Sybren1,5,6,7
关键词: RCA identification ; Fusarium Head Blight ; Fusarium graminearum species complex (FGSC) ; Fusarium incarnatum-equiseti species complex (FIESC) ; Mycotoxins ; Gramineae
刊名: JOURNAL OF MICROBIOLOGICAL METHODS
发表日期: 2012-04-01
DOI: 10.1016/j.mimet.2012.01.017
卷: 89, 期:1, 页:63-70
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Microbiology
研究领域[WOS]: Biochemistry & Molecular Biology ; Microbiology
关键词[WOS]: HEAD BLIGHT PATHOGEN ; DNA-SEQUENCE DATABASE ; GENEALOGICAL CONCORDANCE ; PADLOCK PROBES ; NORTH-AMERICA ; WHEAT ; MYCOTOXINS ; CEREALS ; BARLEY ; POPULATIONS
英文摘要:

Rolling Circle Amplification (RCA) of DNA is a sensitive and cost effective method for the rapid identification of pathogenic fungi without the need for sequencing. Amplification products can be visualized on 1% agarose gel to verify the specificity of probe-template binding or directly by adding fluorescent dyes. Fusarium Head Blight (FHB) is currently the world′s largest threat to the production of cereal crops with the production of a range of mycotoxins as an additional risk. We designed sets of RCA padlock probes based on polymorphisms in the elongation factor 1-alpha (EF-1 alpha) gene to detect the dominant FHB species, comprising lineages of the Fusarium graminearum species complex (FGSC). The method also enabled the identification of species of the Fusarium oxysporum (FOSC), the Fusarium incarnatum-equiseti (FIESC), and the Fusarium tricinctum (FTSC) species complexes, and used strains from the CBS culture collection as reference. Subsequently probes were applied to characterize isolates from wheat and wild grasses, and inoculated wheat kernels. The RCA assays successfully amplified DNA of the target fungi, both in environmental samples and in the contaminated wheat samples, while no cross reactivity was observed with uncontaminated what or related Fusarium species. As RCA does not require expensive instrumentation, the technique has a good potential for local and point of care screening for toxigenic Fusarium species in cereals. (C) 2012 Elsevier B.V. All rights reserved.

语种: 英语
项目资助者: Iranian Ministry of Science, Research and Technology, University of Tabriz
WOS记录号: WOS:000302760700012
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/52853
Appears in Collections:北京大学第一临床医学院_皮肤性病科_期刊论文

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作者单位: 1.CBS KNAW Fungal Biodivers Ctr, NL-3584 CT Utrecht, Netherlands
2.Univ Tabriz, Dept Plant Pathol, Fac Agr, Tabriz, Iran
3.Mashhad Univ Med Sci, Dept Parasitol & Mycol, Ghaem Hosp, Sch Med, Mashhad, Iran
4.Plant Res Int, Biointeract & Plant Hlth, NL-6708 PB Wageningen, Netherlands
5.Univ Amsterdam, Inst Biodivers & Ecosyst Dynam, Amsterdam, Netherlands
6.Peking Univ, Hlth Sci Ctr, Res Ctr Med Mycol, Beijing 100871, Peoples R China
7.Sun Yat Sen Univ, Sun Yat Sen Mem Hosp, Guangzhou 510275, Guangdong, Peoples R China

Recommended Citation:
Davari, Mahdi,van Diepeningen, Anne D.,Babai-Ahari, Assadollah,et al. Rapid identification of Fusarium graminearum species complex using Rolling Circle Amplification (RCA)[J]. JOURNAL OF MICROBIOLOGICAL METHODS,2012,89(1):63-70.
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