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学科主题: 临床医学
题名:
Cdc42 expression in cervical cancer and its effects on cervical tumor invasion and migration
作者: Ye, Hongnan1; Zhang, Youyi2,3; Geng, Li1; Li, Zijian2,3
关键词: Cdc42 ; cervical cancer ; pseudopodia ; invasion ; migration
刊名: INTERNATIONAL JOURNAL OF ONCOLOGY
发表日期: 2015-02-01
DOI: 10.3892/ijo.2014.2748
卷: 46, 期:2, 页:757-763
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology
研究领域[WOS]: Oncology
关键词[WOS]: RHO-GTPASES ; CELL-MIGRATION ; IN-VIVO ; INVADOPODIA ; ACTIVATION ; MECHANISMS ; METASTASIS ; BIOLOGY ; FAMILY ; HPV
英文摘要:

The aim of the present study was to examine Cdc42 expression in cervical cancer and explore its effects on invasion and migration capability of cervical cancer cells. Immunohistochemistry was used to detect Cdc42 expression in normal cervical tissues as well as CIN I or below, CIN II or above, and cervical cancer tissues. Western blot analysis was used to explore Cdc42 expression in normal cervical cell line Cr1-2614 and cervical cancer cell line He La. Plasmids of constitutively active Cdc42 (Cdc42 CA), wild-type Cdc42 (Cdc42 WT) and dominant negative Cdc42 (Cdc42 DN) were transfected, respectively, into He La cells to investigate the impacts of Cdc42 on migration and invasion of cervical cancer cells using Transwell and on cytoskeleton microfilaments using confocal microscopy after immunofluorescence staining. Cdc42 expression was gradually increased in the order of cervical tissues with CIN I or below, CIN II or above and cancer, showing significant difference (P<0.05), and was significantly higher in He La cells than in Cr1-2614 cells (P<0.05). Migration ability of He La cells transfected with Cdc42 CA was significantly higher than that of non-transfected, as well as Cdc42 WT- or Cdc42 DN-transfected He La cells (P<0.05). Overexpression of Cdc42 CA can promote filopodia formation in He La cells. We concluded that Cdc42 overexpression significantly improved the ability of cervical cancer cells to migrate possibly due to improved pseudopodia formation.

语种: 英语
所属项目编号: 81472429 ; 2013CB933702
项目资助者: National Natural Science Foundation of China ; National Basic Research Program of China (973 Program)
WOS记录号: WOS:000347671200036
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/52864
Appears in Collections:北京大学第三临床医学院_期刊论文

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作者单位: 1.Peking Univ, Hosp 3, Dept Gynecol & Obstet, Beijing 100191, Peoples R China
2.Peking Univ, Hosp 4, Inst Vasc Med, Beijing 100191, Peoples R China
3.Minist Hlth, Key Lab Cardiovasc Mol Biol & Regulatory Peptide, Beijing 100191, Peoples R China

Recommended Citation:
Ye, Hongnan,Zhang, Youyi,Geng, Li,et al. Cdc42 expression in cervical cancer and its effects on cervical tumor invasion and migration[J]. INTERNATIONAL JOURNAL OF ONCOLOGY,2015,46(2):757-763.
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