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学科主题临床医学
Cdc42 expression in cervical cancer and its effects on cervical tumor invasion and migration
Ye, Hongnan1; Zhang, Youyi2,3; Geng, Li1; Li, Zijian2,3
关键词Cdc42 Cervical Cancer Pseudopodia Invasion Migration
刊名INTERNATIONAL JOURNAL OF ONCOLOGY
2015-02-01
DOI10.3892/ijo.2014.2748
46期:2页:757-763
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology
资助者National Natural Science Foundation of China ; National Basic Research Program of China (973 Program) ; National Natural Science Foundation of China ; National Basic Research Program of China (973 Program)
研究领域[WOS]Oncology
关键词[WOS]RHO-GTPASES ; CELL-MIGRATION ; IN-VIVO ; INVADOPODIA ; ACTIVATION ; MECHANISMS ; METASTASIS ; BIOLOGY ; FAMILY ; HPV
英文摘要

The aim of the present study was to examine Cdc42 expression in cervical cancer and explore its effects on invasion and migration capability of cervical cancer cells. Immunohistochemistry was used to detect Cdc42 expression in normal cervical tissues as well as CIN I or below, CIN II or above, and cervical cancer tissues. Western blot analysis was used to explore Cdc42 expression in normal cervical cell line Cr1-2614 and cervical cancer cell line He La. Plasmids of constitutively active Cdc42 (Cdc42 CA), wild-type Cdc42 (Cdc42 WT) and dominant negative Cdc42 (Cdc42 DN) were transfected, respectively, into He La cells to investigate the impacts of Cdc42 on migration and invasion of cervical cancer cells using Transwell and on cytoskeleton microfilaments using confocal microscopy after immunofluorescence staining. Cdc42 expression was gradually increased in the order of cervical tissues with CIN I or below, CIN II or above and cancer, showing significant difference (P<0.05), and was significantly higher in He La cells than in Cr1-2614 cells (P<0.05). Migration ability of He La cells transfected with Cdc42 CA was significantly higher than that of non-transfected, as well as Cdc42 WT- or Cdc42 DN-transfected He La cells (P<0.05). Overexpression of Cdc42 CA can promote filopodia formation in He La cells. We concluded that Cdc42 overexpression significantly improved the ability of cervical cancer cells to migrate possibly due to improved pseudopodia formation.

语种英语
所属项目编号81472429 ; 2013CB933702
资助者National Natural Science Foundation of China ; National Basic Research Program of China (973 Program) ; National Natural Science Foundation of China ; National Basic Research Program of China (973 Program)
WOS记录号WOS:000347671200036
引用统计
被引频次:6[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/52864
专题北京大学第三临床医学院
作者单位1.Peking Univ, Hosp 3, Dept Gynecol & Obstet, Beijing 100191, Peoples R China
2.Peking Univ, Hosp 4, Inst Vasc Med, Beijing 100191, Peoples R China
3.Minist Hlth, Key Lab Cardiovasc Mol Biol & Regulatory Peptide, Beijing 100191, Peoples R China
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GB/T 7714
Ye, Hongnan,Zhang, Youyi,Geng, Li,et al. Cdc42 expression in cervical cancer and its effects on cervical tumor invasion and migration[J]. INTERNATIONAL JOURNAL OF ONCOLOGY,2015,46(2):757-763.
APA Ye, Hongnan,Zhang, Youyi,Geng, Li,&Li, Zijian.(2015).Cdc42 expression in cervical cancer and its effects on cervical tumor invasion and migration.INTERNATIONAL JOURNAL OF ONCOLOGY,46(2),757-763.
MLA Ye, Hongnan,et al."Cdc42 expression in cervical cancer and its effects on cervical tumor invasion and migration".INTERNATIONAL JOURNAL OF ONCOLOGY 46.2(2015):757-763.
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