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学科主题基础医学
IL-22 Ameliorates Renal Ischemia-Reperfusion Injury by Targeting Proximal Tubule Epithelium
Xu, Ming-Jiang1,2; Feng, Dechun1; Wang, Hua1; Guan, Youfei2; Yan, Xiaoqiang3; Gao, Bin1
刊名JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY
2014-05-01
25期:5页:967-977
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Urology & Nephrology
研究领域[WOS]Urology & Nephrology
关键词[WOS]ACUTE KIDNEY INJURY ; FAILURE ; CELLS ; APOPTOSIS ; PATHWAYS ; JAK/STAT ; PATHOPHYSIOLOGY ; PROLIFERATION ; EPIDEMIOLOGY ; ACTIVATION
英文摘要

The glomerular basement membrane (GBM) is a specialized extracellular matrix (ECM) compartment within the glomerulus that contains tissue-restricted isoforms of collagen IV and laminin. It is integral to the capillary wall and therefore, functionally linked to glomerular filtration. Although the composition of the GBM has been investigated with global and candidate-based approaches, the relative contributions of glomerular cell types to the production of ECM are not well understood. To characterize specific cellular contributions to the GBM, we used mass spectrometry-based proteomics to analyze ECM isolated from podocytes and glomerular endothelial cells in vitro. These analyses identified cell type-specific differences in ECM composition, indicating distinct contributions to glomerular ECM assembly. Coculture of podocytes and endothelial cells resulted in an altered composition and organization of ECM compared with monoculture ECMs, and electron microscopy revealed basement membrane-like ECM deposition between cocultured cells, suggesting the involvement of cell-cell cross-talk in the production of glomerular ECM. Notably, compared with monoculture ECM proteomes, the coculture ECM proteome better resembled a tissue-derived glomerular ECM dataset, indicating its relevance to GBM in vivo. Protein network analyses revealed a common core of 35 highly connected structural ECM proteins that may be important for glomerular ECM assembly. Overall, these findings show the complexity of the glomerular ECM and suggest that both ECM composition and organization are context-dependent.

语种英语
WOS记录号WOS:000335583700016
项目编号81270370
资助机构National Institute of Alcohol Abuse and Alcoholism ; National Institutes of Health ; National Natural Science Foundation of China
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被引频次:30[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/52876
专题北京大学基础医学院_心血管所
作者单位1.Generon Corp, Shanghai, Peoples R China
2.NIAAA, Lab Liver Dis, NIH, Bethesda, MD 20892 USA
3.Peking Univ, Key Lab Mol Cardiovasc Sci, Dept Physiol & Pathophysiol, Sch Basic Med Sci,Hlth Sci Ctr,Minist Educ, Beijing 100871, Peoples R China
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Xu, Ming-Jiang,Feng, Dechun,Wang, Hua,et al. IL-22 Ameliorates Renal Ischemia-Reperfusion Injury by Targeting Proximal Tubule Epithelium[J]. JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY,2014,25(5):967-977.
APA Xu, Ming-Jiang,Feng, Dechun,Wang, Hua,Guan, Youfei,Yan, Xiaoqiang,&Gao, Bin.(2014).IL-22 Ameliorates Renal Ischemia-Reperfusion Injury by Targeting Proximal Tubule Epithelium.JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY,25(5),967-977.
MLA Xu, Ming-Jiang,et al."IL-22 Ameliorates Renal Ischemia-Reperfusion Injury by Targeting Proximal Tubule Epithelium".JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY 25.5(2014):967-977.
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