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学科主题: 基础医学
题名:
IL-22 Ameliorates Renal Ischemia-Reperfusion Injury by Targeting Proximal Tubule Epithelium
作者: Xu, Ming-Jiang1,2; Feng, Dechun1; Wang, Hua1; Guan, Youfei2; Yan, Xiaoqiang3; Gao, Bin1
刊名: JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY
发表日期: 2014-05-01
卷: 25, 期:5, 页:967-977
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Urology & Nephrology
研究领域[WOS]: Urology & Nephrology
关键词[WOS]: ACUTE KIDNEY INJURY ; FAILURE ; CELLS ; APOPTOSIS ; PATHWAYS ; JAK/STAT ; PATHOPHYSIOLOGY ; PROLIFERATION ; EPIDEMIOLOGY ; ACTIVATION
英文摘要:

The glomerular basement membrane (GBM) is a specialized extracellular matrix (ECM) compartment within the glomerulus that contains tissue-restricted isoforms of collagen IV and laminin. It is integral to the capillary wall and therefore, functionally linked to glomerular filtration. Although the composition of the GBM has been investigated with global and candidate-based approaches, the relative contributions of glomerular cell types to the production of ECM are not well understood. To characterize specific cellular contributions to the GBM, we used mass spectrometry-based proteomics to analyze ECM isolated from podocytes and glomerular endothelial cells in vitro. These analyses identified cell type-specific differences in ECM composition, indicating distinct contributions to glomerular ECM assembly. Coculture of podocytes and endothelial cells resulted in an altered composition and organization of ECM compared with monoculture ECMs, and electron microscopy revealed basement membrane-like ECM deposition between cocultured cells, suggesting the involvement of cell-cell cross-talk in the production of glomerular ECM. Notably, compared with monoculture ECM proteomes, the coculture ECM proteome better resembled a tissue-derived glomerular ECM dataset, indicating its relevance to GBM in vivo. Protein network analyses revealed a common core of 35 highly connected structural ECM proteins that may be important for glomerular ECM assembly. Overall, these findings show the complexity of the glomerular ECM and suggest that both ECM composition and organization are context-dependent.

语种: 英语
所属项目编号: 81270370
项目资助者: National Institute of Alcohol Abuse and Alcoholism ; National Institutes of Health ; National Natural Science Foundation of China
WOS记录号: WOS:000335583700016
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/52876
Appears in Collections:基础医学院_心血管所_期刊论文

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作者单位: 1.Generon Corp, Shanghai, Peoples R China
2.NIAAA, Lab Liver Dis, NIH, Bethesda, MD 20892 USA
3.Peking Univ, Key Lab Mol Cardiovasc Sci, Dept Physiol & Pathophysiol, Sch Basic Med Sci,Hlth Sci Ctr,Minist Educ, Beijing 100871, Peoples R China

Recommended Citation:
Xu, Ming-Jiang,Feng, Dechun,Wang, Hua,et al. IL-22 Ameliorates Renal Ischemia-Reperfusion Injury by Targeting Proximal Tubule Epithelium[J]. JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY,2014,25(5):967-977.
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