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学科主题基础医学
Molecular cloning and characterization of a novel rat CXC chemokine rPBP, the homologue of human and mouse PBP
Fu, WX; Zhu, ML; Gong, SY; Li, Y; Chen, WF
关键词chemokine chemotaxis EST assembly PBP
刊名CYTOKINE
2004-04-07
DOI10.1016/j.cyto.2003.12.007
26期:1页:37-43
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Cell Biology ; Immunology
研究领域[WOS]Biochemistry & Molecular Biology ; Cell Biology ; Immunology
关键词[WOS]PLATELET BASIC-PROTEIN ; MACROPHAGE-INFLAMMATORY PROTEIN-1 ; BETA-THROMBOGLOBULIN ; GENE ; EXPRESSION ; ENDOTOXIN ; FACTOR-4 ; CELLS
英文摘要

We have previously cloned the mouse platelet basic protein (mPBP), a homologue of human PBP, from mouse thymic stromal cells. Using EST alignment and RT-PCR, the rat homologue of human and mouse PBP was cloned from lung and named as rPBP. The complete open reading frame and part of the 3′- and 5′-non-coding regions were obtained through rapid amplification of cDNA ends. The rPBP cDNA encodes a protein of 111 amino acids containing a signal peptide of 37 amino acids at the N-terminus, with the mature protein of 74 amino acids. The rPBP is a new member of ELR+CXC chemokines. The mature protein of rPBP shares 69% and 45% homology with mouse and human PBP, respectively. In situ hybridization assay revealed rPBP to be predominantly localized in the pulmonary vascular endothelial cells. The eukaryotic expression vector pCDNA3-rPBP was constructed and transiently transfected into COS-7 cells. In the in vitro chemotaxis assay, the polymorphonuclear leukocytes (PMNs) were chemoattracted to the supernatants from transfected COS-7 cells in a dose-dependent manner. The implication of rPBP found in rat lung is that this chemokine may have the function to recruit PMNs to fight against pulmonary infection. (C) 2004 Elsevier Ltd. All rights reserved.

语种英语
WOS记录号WOS:000220635900006
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被引频次:2[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/52880
专题基础医学院_免疫学系
作者单位Peking Univ, Hlth Sci Ctr, Dept Immunol, Beijing 100083, Peoples R China
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Fu, WX,Zhu, ML,Gong, SY,et al. Molecular cloning and characterization of a novel rat CXC chemokine rPBP, the homologue of human and mouse PBP[J]. CYTOKINE,2004,26(1):37-43.
APA Fu, WX,Zhu, ML,Gong, SY,Li, Y,&Chen, WF.(2004).Molecular cloning and characterization of a novel rat CXC chemokine rPBP, the homologue of human and mouse PBP.CYTOKINE,26(1),37-43.
MLA Fu, WX,et al."Molecular cloning and characterization of a novel rat CXC chemokine rPBP, the homologue of human and mouse PBP".CYTOKINE 26.1(2004):37-43.
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