北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 基础医学院  > 期刊论文
学科主题: 基础医学
题名:
p53-Induced Uncoupling Expression of Aquaporin-4 and Inwardly Rectifying K+ 4.1 Channels in Cytotoxic Edema after Subarachnoid Hemorrhage
作者: Yan, Jun-hao1,2; Khatibi, Nikan H.3; Han, Hong-bin4; Hu, Qin2; Chen, Chun-hua1,2; Li, Li1; Yang, Xiao-mei1; Zhou, Chang-man1
关键词: Astrocyte ; Cytotoxic edema ; p53 ; Rat ; Subarachnoid hemorrhage
刊名: CNS NEUROSCIENCE & THERAPEUTICS
发表日期: 2012
DOI: 10.1111/j.1755-5949.2012.00299.x
卷: 18, 期:4, 页:334-342
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Neurosciences ; Pharmacology & Pharmacy
研究领域[WOS]: Neurosciences & Neurology ; Pharmacology & Pharmacy
关键词[WOS]: EARLY BRAIN-INJURY ; CEREBRAL-ISCHEMIA ; PIFITHRIN-ALPHA ; RAT MODEL ; P53 ; ACTIVATION ; ASTROCYTES ; VASOSPASM ; APOPTOSIS ; MICE
英文摘要:

Aims: To investigate the mechanism behind cytotoxic edema formation following subarachnoid hemorrhage (SAH). Methods: We explored the role of aquaporin-4 (AQP4), inwardly rectifying K+ 4.1 (Kir4.1) channels and their upstream orchestrators p53 and p38MAPK in this process. A p53 inhibitor, pifithrin-a (PFT-a) was administered intraperitoneally to rats undergoing SAH by endovascular perforation. Totally, 98 male SD rats were categorized into sham, SAH, SAH+ dimethyl sulfoxide (DMSO), SAH+ 0.2 or 2.0 mg/kg PFT-a groups. At 24 h after SAH, MRI (diffusion-weighted imaging [DWI]), immunohistochemistry, and Western blot were used. Results: MRI (DWI) showed a significant cytotoxic edema in the brain following SAH with PFT-a therapy reducing it. Immunohistochemistry and Western blot showed an increased level of p53, phosphorylated-p38MAPK and AQP4 and a reduced level of Kir4.1; all of which could be reversed following PFT-a treatment. Treble labeling staining revealed colocalization of p53 with phosphorylated-p38MAPK and unmatched expression of AQP4 and Kir4.1 within astrocyte cells. Conclusion: These results indicated p53 mediates the formation of cytotoxic edema in the brain following SAH; an uncoupling expression of AQP4 and Kir4.1 on astrocytic end feets orchestrated by p38MAPK was partly responsible.

语种: 英语
所属项目编号: 30901548 ; 30971527
项目资助者: National Natural Science Foundation of China
WOS记录号: WOS:000302355000009
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/52888
Appears in Collections:基础医学院_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Loma Linda Univ, Med Ctr, Dept Anesthesiol, Loma Linda, CA USA
2.Peking Univ, Dept Anat & Embryol, Sch Basic Med Sci, Beijing 100191, Peoples R China
3.Loma Linda Univ, Med Ctr, Dept Physiol & Pharmacol, Loma Linda, CA USA
4.Peking Univ, Dept Radiol, Hosp 3, Beijing 100191, Peoples R China

Recommended Citation:
Yan, Jun-hao,Khatibi, Nikan H.,Han, Hong-bin,et al. p53-Induced Uncoupling Expression of Aquaporin-4 and Inwardly Rectifying K+ 4.1 Channels in Cytotoxic Edema after Subarachnoid Hemorrhage[J]. CNS NEUROSCIENCE & THERAPEUTICS,2012,18(4):334-342.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Yan, Jun-hao]'s Articles
[Khatibi, Nikan H.]'s Articles
[Han, Hong-bin]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Yan, Jun-hao]‘s Articles
[Khatibi, Nikan H.]‘s Articles
[Han, Hong-bin]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace