A near infrared fluorescent/ultrasonic bimodal contrast agent for imaging guided pDNA delivery via ultrasound targeted microbubble destruction | |
Yang, Yongbo2; Wang, Jinrui3; Li, Xiaoda2; Lin, Li2; Yue, Xiuli1 | |
Source Publication | RSC ADVANCES
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2015 | |
Volume | 5Issue:11Pages:8404-8414 |
Indexed By | SCI |
Abstract | This paper reported the development of a multifunctional contrast agent for near infrared (NIR) fluorescent/ultrasonic bimodal imaging and gene delivery. CuInS2-ZnS alloyed quantum dots (ZCIS QDs) were coated with polyethyleneimine (PEI) to complex plasmid DNA (pDNA), followed by adsorption onto the surface of microbubbles (MBs) generated from the surfactant mixture of Span 60 and Tween 80. It was found that the obtained composite agent of MBs@QDs@PEI/pDNA had excellent capability to enhance both ultrasound and fluorescence imaging. In addition, an in vitro cell experiment showed that pDNA could be released from MBs@QDs@PEI/pDNA and internalized by target HeLa cells to realize a relatively high transfection efficiency by ultrasound-targeted microbubble destruction. Furthermore, the cytotoxicity, immune toxicity and histological evaluation showed that MBs@QDs@PEI/pDNA had a good biocompatibility for medical application. Therefore, such a multifunction agent could operate as a promising platform for targeting gene delivery under the guidance of NIR fluorescent/ultrasonic bimodal imaging. |
Subject Area | 临床医学 |
Subtype | Article |
DOI | 10.1039/c4ra15066j |
WOS Headings | Science & Technology |
Language | 英语 |
Funding Organization | National Natural Science Foundation of China ; State Key Program of National Natural Science of China |
WOS Research Area | Chemistry |
WOS Subject | Chemistry, Multidisciplinary |
WOS Keyword | SEMICONDUCTOR QUANTUM DOTS ; IN-VIVO ; GENE DELIVERY ; PHOTOTHERMAL THERAPY ; SIRNA DELIVERY ; NANOPARTICLES ; POLYETHYLENIMINE ; NANOCRYSTALS ; TRANSFECTION ; EFFICIENCY |
WOS ID | WOS:000347720900084 |
Citation statistics | |
Document Type | 期刊论文 |
Identifier | http://ir.bjmu.edu.cn/handle/400002259/52901 |
Collection | 北京大学第三临床医学院_超声诊断科 北京大学临床肿瘤学院_肝胆胰外二科 北京大学临床肿瘤学院_胸部肿瘤外一科 |
Affiliation | 1.Harbin Inst Technol, Sch Municipal & Environm Engn, Harbin 150001, Peoples R China 2.Harbin Inst Technol, Sch Life Sci & Technol, Harbin 150001, Peoples R China 3.Peking Univ, Hosp 3, Dept Ultrasonog, Beijing 100083, Peoples R China |
Recommended Citation GB/T 7714 | Yang, Yongbo,Wang, Jinrui,Li, Xiaoda,et al. A near infrared fluorescent/ultrasonic bimodal contrast agent for imaging guided pDNA delivery via ultrasound targeted microbubble destruction[J]. RSC ADVANCES,2015,5(11):8404-8414. |
APA | Yang, Yongbo,Wang, Jinrui,Li, Xiaoda,Lin, Li,&Yue, Xiuli.(2015).A near infrared fluorescent/ultrasonic bimodal contrast agent for imaging guided pDNA delivery via ultrasound targeted microbubble destruction.RSC ADVANCES,5(11),8404-8414. |
MLA | Yang, Yongbo,et al."A near infrared fluorescent/ultrasonic bimodal contrast agent for imaging guided pDNA delivery via ultrasound targeted microbubble destruction".RSC ADVANCES 5.11(2015):8404-8414. |
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