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A near infrared fluorescent/ultrasonic bimodal contrast agent for imaging guided pDNA delivery via ultrasound targeted microbubble destruction
Yang, Yongbo2; Wang, Jinrui3; Li, Xiaoda2; Lin, Li2; Yue, Xiuli1
Source PublicationRSC ADVANCES
2015
Volume5Issue:11Pages:8404-8414
Indexed BySCI
Abstract

This paper reported the development of a multifunctional contrast agent for near infrared (NIR) fluorescent/ultrasonic bimodal imaging and gene delivery. CuInS2-ZnS alloyed quantum dots (ZCIS QDs) were coated with polyethyleneimine (PEI) to complex plasmid DNA (pDNA), followed by adsorption onto the surface of microbubbles (MBs) generated from the surfactant mixture of Span 60 and Tween 80. It was found that the obtained composite agent of MBs@QDs@PEI/pDNA had excellent capability to enhance both ultrasound and fluorescence imaging. In addition, an in vitro cell experiment showed that pDNA could be released from MBs@QDs@PEI/pDNA and internalized by target HeLa cells to realize a relatively high transfection efficiency by ultrasound-targeted microbubble destruction. Furthermore, the cytotoxicity, immune toxicity and histological evaluation showed that MBs@QDs@PEI/pDNA had a good biocompatibility for medical application. Therefore, such a multifunction agent could operate as a promising platform for targeting gene delivery under the guidance of NIR fluorescent/ultrasonic bimodal imaging.

Subject Area临床医学
SubtypeArticle
DOI10.1039/c4ra15066j
WOS HeadingsScience & Technology
Language英语
Funding OrganizationNational Natural Science Foundation of China ; State Key Program of National Natural Science of China
WOS Research AreaChemistry
WOS SubjectChemistry, Multidisciplinary
WOS KeywordSEMICONDUCTOR QUANTUM DOTS ; IN-VIVO ; GENE DELIVERY ; PHOTOTHERMAL THERAPY ; SIRNA DELIVERY ; NANOPARTICLES ; POLYETHYLENIMINE ; NANOCRYSTALS ; TRANSFECTION ; EFFICIENCY
WOS IDWOS:000347720900084
Citation statistics
Cited Times:7[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.bjmu.edu.cn/handle/400002259/52901
Collection北京大学第三临床医学院_超声诊断科
北京大学临床肿瘤学院_肝胆胰外二科
北京大学临床肿瘤学院_胸部肿瘤外一科
Affiliation1.Harbin Inst Technol, Sch Municipal & Environm Engn, Harbin 150001, Peoples R China
2.Harbin Inst Technol, Sch Life Sci & Technol, Harbin 150001, Peoples R China
3.Peking Univ, Hosp 3, Dept Ultrasonog, Beijing 100083, Peoples R China
Recommended Citation
GB/T 7714
Yang, Yongbo,Wang, Jinrui,Li, Xiaoda,et al. A near infrared fluorescent/ultrasonic bimodal contrast agent for imaging guided pDNA delivery via ultrasound targeted microbubble destruction[J]. RSC ADVANCES,2015,5(11):8404-8414.
APA Yang, Yongbo,Wang, Jinrui,Li, Xiaoda,Lin, Li,&Yue, Xiuli.(2015).A near infrared fluorescent/ultrasonic bimodal contrast agent for imaging guided pDNA delivery via ultrasound targeted microbubble destruction.RSC ADVANCES,5(11),8404-8414.
MLA Yang, Yongbo,et al."A near infrared fluorescent/ultrasonic bimodal contrast agent for imaging guided pDNA delivery via ultrasound targeted microbubble destruction".RSC ADVANCES 5.11(2015):8404-8414.
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