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Computational modeling and functional analysis of Herpes simplex virus type-1 thymidine kinase and Escherichia coli cytosine deaminase fusion protein
Zhang, Jufeng1; Wang, Zhanli1; Wei, Fang1; Qiu, Wei1; Zhang, Liangren1; Huang, Qian1
关键词Molecular Modeling Fusion Peptide Cytosine Deaminase Herpes Simplex Virus Type-1 Thymidine Kinase
刊名BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
2007-08-17
DOI10.1016/j.bbrc.2007.05.220
360期:1页:46-50
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]SUICIDE GENE-THERAPY ; GLIOMA-CELLS ; 5-FLUOROCYTOSINE ; CYTOTOXICITY ; ADENOVIRUS ; RECEPTOR ; GANCICLOVIR ; PREDICTION ; PEPTIDES
英文摘要

Herpes simplex virus type-1 thymidine kinase (HSV-1TK) and Escherichia coli cytosine deaminase (CD) fusion protein was designed using InsightII software. The structural rationality of the fusion proteins incorporating a series of flexible linker peptide was analyzed, and a suitable linker peptide was chosen for further investigated. The recombinant plasmid containing the coding regions of HSV-1TK and CD cDNA connected by this linker peptide coding sequence was generated and subsequently transfected into the human embryonic kidney 293 cells (HEK293). The Western blotting indicated that the recombinant fusion protein existed as a dimer with a molecular weight of approximately 90 kDa. The toxicity of the prodrug on the recombinant plasmid-transfected human lung cancer cell line NCIH460 was evaluated, which showed that TKglyCD-expressing cells conferred upon cells prodrug sensitivities equivalent to that observed for each enzyme independently. Most noteworthy, cytotoxicity could be enhanced by concurrently treating TKglyCD-expressing cells with prodrugs GCV and 5-FC. The results indicate that we have successfully constructed a HSV-1TKglyCD fusion gene which might have a potential application for cancer gene therapy. (c) 2007 Elsevier Inc. All rights reserved.

语种英语
WOS记录号WOS:000248137400008
引用统计
被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/52902
专题北京大学药学院
作者单位1.Shanghai Jiao Tong Univ, Peoples Hosp 1, Cent Expt Lab, Shanghai 200080, Peoples R China
2.NeoTrident Technol LTd, Ctr Technol, Beijing 100080, Peoples R China
3.Peking Univ, Sch Pharmaceut Sci, Beijing 100083, Peoples R China
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Zhang, Jufeng,Wang, Zhanli,Wei, Fang,et al. Computational modeling and functional analysis of Herpes simplex virus type-1 thymidine kinase and Escherichia coli cytosine deaminase fusion protein[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2007,360(1):46-50.
APA Zhang, Jufeng,Wang, Zhanli,Wei, Fang,Qiu, Wei,Zhang, Liangren,&Huang, Qian.(2007).Computational modeling and functional analysis of Herpes simplex virus type-1 thymidine kinase and Escherichia coli cytosine deaminase fusion protein.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,360(1),46-50.
MLA Zhang, Jufeng,et al."Computational modeling and functional analysis of Herpes simplex virus type-1 thymidine kinase and Escherichia coli cytosine deaminase fusion protein".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 360.1(2007):46-50.
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