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学科主题: 临床医学
题名:
Calcium phosphate nanoparticles as a novel nonviral vector for efficient trasfection of DNA in cancer gene therapy
作者: Liu, T1; Tang, A1; Zhang, GY1; Chen, YX1; Zhang, JY1; Peng, SS1; Cai, ZM1
关键词: calcium phosphate nanoparticles (CPNP) ; nonviral vector ; gene transfer ; nasopharyngeal carcinoma (NPC)
刊名: CANCER BIOTHERAPY AND RADIOPHARMACEUTICALS
发表日期: 2005-04-01
卷: 20, 期:2, 页:141-149
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology ; Medicine, Research & Experimental ; Pharmacology & Pharmacy ; Radiology, Nuclear Medicine & Medical Imaging
研究领域[WOS]: Oncology ; Research & Experimental Medicine ; Pharmacology & Pharmacy ; Radiology, Nuclear Medicine & Medical Imaging
关键词[WOS]: NASOPHARYNGEAL CARCINOMA ; PHASE-I ; DELIVERY ; TRANSFECTION ; NANOSPHERES ; OVARIAN ; CELLS ; ADENOVIRUSES ; PROSTATE ; MODEL
英文摘要:

To explore an efficient gene vector in cancer gene therapy, a novel nonviral vector calcium phosphate nanoparticle (CPNP) was developed. Transmission electromicroscopy and Zeta potential demonstrated that CPNP was 23.5-34.5 nm diameters and had + 16.8 mV positive surface charges. The analysis of the CPNP-DNA complex showed CPNP could transfer foreign DNA. into targeted cells with high transfection efficiency as well as its DNA-binding property and protection of DNA from degradation. Furthermore, the CPNP-DNA complex had no obvious cytotoxicity for CNE-2 cells, while the liposome-DNA complex had certain cytotoxicity. When the CPNP combined with suicide genes yCDglyTK for nasopharyngeal carcinoma (NPC) therapy in vitro, just 24.76% (of cells survived when the wild-type CNE-2 cells were treated. with the CPNP-yCDglyTK complex plus the prodrug, 5-FC (200 mg/mL). Otherwise, the expression of yCDglyTK was detected in implanted CNE-2 tumors by reverse transcription-polymerase chain reaction (RT-PCR) analysis when the CNE-2 tumor was treated with an intratumoral injection of the CPNP-yCDglyTK complex. Our results showed that the CPNP might be a potential vector for gene therapy.

语种: 英语
WOS记录号: WOS:000229273500004
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/52909
Appears in Collections:北京大学深圳医院_生殖医学科_期刊论文

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作者单位: 1.Cent S Univ, Xiangya Hosp, Changsha 410078, Peoples R China
2.Shenzhen Peoples Hosp, Dept Pathol, Shenzhen, Peoples R China
3.Cent S Univ, Sch Biosci & Technol, Changsha 410078, Peoples R China
4.Peking Univ, Shenzhen Hosp, Reprod Med Ctr, Shenzhen, Peoples R China

Recommended Citation:
Liu, T,Tang, A,Zhang, GY,et al. Calcium phosphate nanoparticles as a novel nonviral vector for efficient trasfection of DNA in cancer gene therapy[J]. CANCER BIOTHERAPY AND RADIOPHARMACEUTICALS,2005,20(2):141-149.
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