IR@PKUHSC  > 北京大学深圳医院  > 生殖医学科
学科主题临床医学
Calcium phosphate nanoparticles as a novel nonviral vector for efficient trasfection of DNA in cancer gene therapy
Liu, T1; Tang, A1; Zhang, GY1; Chen, YX1; Zhang, JY1; Peng, SS1; Cai, ZM1
关键词Calcium Phosphate Nanoparticles (Cpnp) Nonviral Vector Gene Transfer Nasopharyngeal Carcinoma (Npc)
刊名CANCER BIOTHERAPY AND RADIOPHARMACEUTICALS
2005-04-01
20期:2页:141-149
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology ; Medicine, Research & Experimental ; Pharmacology & Pharmacy ; Radiology, Nuclear Medicine & Medical Imaging
研究领域[WOS]Oncology ; Research & Experimental Medicine ; Pharmacology & Pharmacy ; Radiology, Nuclear Medicine & Medical Imaging
关键词[WOS]NASOPHARYNGEAL CARCINOMA ; PHASE-I ; DELIVERY ; TRANSFECTION ; NANOSPHERES ; OVARIAN ; CELLS ; ADENOVIRUSES ; PROSTATE ; MODEL
英文摘要

To explore an efficient gene vector in cancer gene therapy, a novel nonviral vector calcium phosphate nanoparticle (CPNP) was developed. Transmission electromicroscopy and Zeta potential demonstrated that CPNP was 23.5-34.5 nm diameters and had + 16.8 mV positive surface charges. The analysis of the CPNP-DNA complex showed CPNP could transfer foreign DNA. into targeted cells with high transfection efficiency as well as its DNA-binding property and protection of DNA from degradation. Furthermore, the CPNP-DNA complex had no obvious cytotoxicity for CNE-2 cells, while the liposome-DNA complex had certain cytotoxicity. When the CPNP combined with suicide genes yCDglyTK for nasopharyngeal carcinoma (NPC) therapy in vitro, just 24.76% (of cells survived when the wild-type CNE-2 cells were treated. with the CPNP-yCDglyTK complex plus the prodrug, 5-FC (200 mg/mL). Otherwise, the expression of yCDglyTK was detected in implanted CNE-2 tumors by reverse transcription-polymerase chain reaction (RT-PCR) analysis when the CNE-2 tumor was treated with an intratumoral injection of the CPNP-yCDglyTK complex. Our results showed that the CPNP might be a potential vector for gene therapy.

语种英语
WOS记录号WOS:000229273500004
引用统计
被引频次:57[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/52909
专题北京大学深圳医院_生殖医学科
作者单位1.Cent S Univ, Xiangya Hosp, Changsha 410078, Peoples R China
2.Shenzhen Peoples Hosp, Dept Pathol, Shenzhen, Peoples R China
3.Cent S Univ, Sch Biosci & Technol, Changsha 410078, Peoples R China
4.Peking Univ, Shenzhen Hosp, Reprod Med Ctr, Shenzhen, Peoples R China
推荐引用方式
GB/T 7714
Liu, T,Tang, A,Zhang, GY,et al. Calcium phosphate nanoparticles as a novel nonviral vector for efficient trasfection of DNA in cancer gene therapy[J]. CANCER BIOTHERAPY AND RADIOPHARMACEUTICALS,2005,20(2):141-149.
APA Liu, T.,Tang, A.,Zhang, GY.,Chen, YX.,Zhang, JY.,...&Cai, ZM.(2005).Calcium phosphate nanoparticles as a novel nonviral vector for efficient trasfection of DNA in cancer gene therapy.CANCER BIOTHERAPY AND RADIOPHARMACEUTICALS,20(2),141-149.
MLA Liu, T,et al."Calcium phosphate nanoparticles as a novel nonviral vector for efficient trasfection of DNA in cancer gene therapy".CANCER BIOTHERAPY AND RADIOPHARMACEUTICALS 20.2(2005):141-149.
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