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学科主题临床医学
Role of recombinant plasmid pEGFP-N1-IGF-1 transfection in alleviating osteoporosis in ovariectomized rats
Liu, Hai Chun1; Zhao, Hua1; Chen, Jian2; Wu, Wen Liang1; Wang, Hong Liang1; Jiao, Gang Jun3; Chen, Yun Zhen1
关键词Igf-1 Recombinant Plasmid Gene Transfection Osteoporosis Rat
刊名JOURNAL OF MOLECULAR HISTOLOGY
2013-10-01
DOI10.1007/s10735-013-9498-3
44期:5页:535-544
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cell Biology
研究领域[WOS]Cell Biology
关键词[WOS]GROWTH-FACTOR ; GENE-EXPRESSION ; MICE ; CELLS ; IGF-1 ; DNA ; FLUORESCENCE ; DELIVERY ; SYSTEM ; LIVER
英文摘要

Decreased levels of serum insulin-like growth factor-1 (IGF-1) have been proven to cause osteoporosis. Gene transfer of IGF-1 offers an attractive technology to treat skeletal metabolic disorders including osteoporosis, but the viral vectors are limited by their high antigenicity and immune response. Our purpose was to investigate the expression of a non-invasive vector, recombinant plasmid enhanced green fluorescent protein-N1 (pEGFP-N1) that transferred IGF-1 gene into ovariectomized (OVX) rats in vivo and evaluate the effect of this therapy on osteoporosis. OVX or sham operations were performed in 60 female, 7-month-old unmated SD rats. 12 weeks after OVX operation, the vectors were transfected to the 10-month-old rats and experimental data were detected from 48 h to 7 week after transfection. Our results showed that remarkable expression of fluorescence and serum IGF-1 was observed in the rats transfected by recombinant plasmids, indicating that IGF-1 gene was successfully transferred to OVX rats by injecting the vector through hydrodynamic method via the tail vein. The bone metabolism index including serum alkaline phosphatase, the histomorphometric parameters of lumbar vertebra including trabecular area percentage, trabecular thickness, trabecular number and trabecular separation, and the bone mineral density (BMD) and biomechanical parameters of lumbar vertebra including BMD, maximum condensing force, crushing strength in OVX rats transfected by pEGFP-N1-IGF-1 were improved remarkably compared with OVX+pEGFP-N1 rats, indicating that the transfection of recombinant plasmid pEGFP-N1-IGF-1 played a significant role in alleviating osteoporosis in rats induced by OVX. This encouraged a potential approach of IGF-1 gene therapy to the treatment of osteoporosis.

语种英语
WOS记录号WOS:000325160700005
引用统计
被引频次:4[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/52913
专题北京大学第二临床医学院_骨关节科
作者单位1.Linyi Peoples Hosp, Dept Hand Surg, Linyi, Peoples R China
2.Shandong Univ, Dept Orthopaed, Qilu Hosp, Jinan 250012, Peoples R China
3.Peking Univ, Dept Orthopaed, Peoples Hosp, Beijing 100871, Peoples R China
推荐引用方式
GB/T 7714
Liu, Hai Chun,Zhao, Hua,Chen, Jian,et al. Role of recombinant plasmid pEGFP-N1-IGF-1 transfection in alleviating osteoporosis in ovariectomized rats[J]. JOURNAL OF MOLECULAR HISTOLOGY,2013,44(5):535-544.
APA Liu, Hai Chun.,Zhao, Hua.,Chen, Jian.,Wu, Wen Liang.,Wang, Hong Liang.,...&Chen, Yun Zhen.(2013).Role of recombinant plasmid pEGFP-N1-IGF-1 transfection in alleviating osteoporosis in ovariectomized rats.JOURNAL OF MOLECULAR HISTOLOGY,44(5),535-544.
MLA Liu, Hai Chun,et al."Role of recombinant plasmid pEGFP-N1-IGF-1 transfection in alleviating osteoporosis in ovariectomized rats".JOURNAL OF MOLECULAR HISTOLOGY 44.5(2013):535-544.
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