北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学第二临床医学院  > 骨关节科  > 期刊论文
学科主题: 临床医学
题名:
Role of recombinant plasmid pEGFP-N1-IGF-1 transfection in alleviating osteoporosis in ovariectomized rats
作者: Liu, Hai Chun1; Zhao, Hua1; Chen, Jian2; Wu, Wen Liang1; Wang, Hong Liang1; Jiao, Gang Jun3; Chen, Yun Zhen1
关键词: IGF-1 ; Recombinant plasmid ; Gene transfection ; Osteoporosis ; Rat
刊名: JOURNAL OF MOLECULAR HISTOLOGY
发表日期: 2013-10-01
DOI: 10.1007/s10735-013-9498-3
卷: 44, 期:5, 页:535-544
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Cell Biology
研究领域[WOS]: Cell Biology
关键词[WOS]: GROWTH-FACTOR ; GENE-EXPRESSION ; MICE ; CELLS ; IGF-1 ; DNA ; FLUORESCENCE ; DELIVERY ; SYSTEM ; LIVER
英文摘要:

Decreased levels of serum insulin-like growth factor-1 (IGF-1) have been proven to cause osteoporosis. Gene transfer of IGF-1 offers an attractive technology to treat skeletal metabolic disorders including osteoporosis, but the viral vectors are limited by their high antigenicity and immune response. Our purpose was to investigate the expression of a non-invasive vector, recombinant plasmid enhanced green fluorescent protein-N1 (pEGFP-N1) that transferred IGF-1 gene into ovariectomized (OVX) rats in vivo and evaluate the effect of this therapy on osteoporosis. OVX or sham operations were performed in 60 female, 7-month-old unmated SD rats. 12 weeks after OVX operation, the vectors were transfected to the 10-month-old rats and experimental data were detected from 48 h to 7 week after transfection. Our results showed that remarkable expression of fluorescence and serum IGF-1 was observed in the rats transfected by recombinant plasmids, indicating that IGF-1 gene was successfully transferred to OVX rats by injecting the vector through hydrodynamic method via the tail vein. The bone metabolism index including serum alkaline phosphatase, the histomorphometric parameters of lumbar vertebra including trabecular area percentage, trabecular thickness, trabecular number and trabecular separation, and the bone mineral density (BMD) and biomechanical parameters of lumbar vertebra including BMD, maximum condensing force, crushing strength in OVX rats transfected by pEGFP-N1-IGF-1 were improved remarkably compared with OVX+pEGFP-N1 rats, indicating that the transfection of recombinant plasmid pEGFP-N1-IGF-1 played a significant role in alleviating osteoporosis in rats induced by OVX. This encouraged a potential approach of IGF-1 gene therapy to the treatment of osteoporosis.

语种: 英语
WOS记录号: WOS:000325160700005
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/52913
Appears in Collections:北京大学第二临床医学院_骨关节科_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Linyi Peoples Hosp, Dept Hand Surg, Linyi, Peoples R China
2.Shandong Univ, Dept Orthopaed, Qilu Hosp, Jinan 250012, Peoples R China
3.Peking Univ, Dept Orthopaed, Peoples Hosp, Beijing 100871, Peoples R China

Recommended Citation:
Liu, Hai Chun,Zhao, Hua,Chen, Jian,et al. Role of recombinant plasmid pEGFP-N1-IGF-1 transfection in alleviating osteoporosis in ovariectomized rats[J]. JOURNAL OF MOLECULAR HISTOLOGY,2013,44(5):535-544.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Liu, Hai Chun]'s Articles
[Zhao, Hua]'s Articles
[Chen, Jian]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Liu, Hai Chun]‘s Articles
[Zhao, Hua]‘s Articles
[Chen, Jian]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace