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学科主题口腔医学
Construction of adipose scaffold for bone repair with gene engineering bone cells
Li, Weiming1; Fan, Jingzhang1; Chen, Feng2; Yang, Weiliang1; Su, Jian1; Bi, Zhenggang1
关键词Bone Defect Bone Repair Transplantation Tissue Engineering Engineering Bone
刊名EXPERIMENTAL BIOLOGY AND MEDICINE
2013-12-01
DOI10.1177/1535370213506677
238期:12页:1350-1354
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Medicine, Research & Experimental
研究领域[WOS]Research & Experimental Medicine
关键词[WOS]MESENCHYMAL STEM-CELLS ; REGENERATION ; EXPRESSION ; DIFFERENTIATION ; PLURIPOTENCY ; DELIVERY
英文摘要

The bone defect repairing is still a challenge in orthopedics. As the gene engineering bones have been used in the bone repairing clinic, the scaffold construction is a critical fact to be considered. This study aims to construct optimal scaffolds using adipose tissue in the bone repair together with the gene engineering osteocytes. Rat adipose stem cells (ASC) were prepared; the cells were transduced with the OCT-4 gene carrying lentiviral vectors (OCT-4-Lv). Artificial bone defects were created in the rat femoral bone. The bone defects were filled up with adipose scaffolds and shaped by using surrounding muscles and supported with orthopedic splints. ASCs with or without transducing the OCT-4-Lv were injected into the adipose scaffolds. The rats were sacrificed 12 weeks after the surgery. After receiving the OCT-4-Lv, the expressions of OCT-4, RUNX2 and osteocalcin were detected in the ASCs. X-ray examination showed that rats received the OCT-4-Lv transduced ASCs together with the adipose pad had new bone formation in the defect area; none of the control rats showed any new bone formation in situ. The results were supported by histological assessment. Using adipose scaffold and OCT-4-modified ASC transplantation can repair bone defects.

语种英语
WOS记录号WOS:000327510800003
项目编号81171692
资助机构China National Natural Science Foundation
引用统计
被引频次:2[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/52920
专题北京大学口腔医学院
作者单位1.Harbin Med Univ, Affiliated Hosp 1, Dept Orthopaed, Harbin 150001, Heilongjiang, Peoples R China
2.Peking Univ, Sch Stomatol, Dept Cent Lab, Beijing 100081, Peoples R China
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Li, Weiming,Fan, Jingzhang,Chen, Feng,et al. Construction of adipose scaffold for bone repair with gene engineering bone cells[J]. EXPERIMENTAL BIOLOGY AND MEDICINE,2013,238(12):1350-1354.
APA Li, Weiming,Fan, Jingzhang,Chen, Feng,Yang, Weiliang,Su, Jian,&Bi, Zhenggang.(2013).Construction of adipose scaffold for bone repair with gene engineering bone cells.EXPERIMENTAL BIOLOGY AND MEDICINE,238(12),1350-1354.
MLA Li, Weiming,et al."Construction of adipose scaffold for bone repair with gene engineering bone cells".EXPERIMENTAL BIOLOGY AND MEDICINE 238.12(2013):1350-1354.
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