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学科主题: 口腔医学
题名:
Construction of adipose scaffold for bone repair with gene engineering bone cells
作者: Li, Weiming1; Fan, Jingzhang1; Chen, Feng2; Yang, Weiliang1; Su, Jian1; Bi, Zhenggang1
关键词: Bone defect ; bone repair ; transplantation ; tissue engineering ; engineering bone
刊名: EXPERIMENTAL BIOLOGY AND MEDICINE
发表日期: 2013-12-01
DOI: 10.1177/1535370213506677
卷: 238, 期:12, 页:1350-1354
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medicine, Research & Experimental
研究领域[WOS]: Research & Experimental Medicine
关键词[WOS]: MESENCHYMAL STEM-CELLS ; REGENERATION ; EXPRESSION ; DIFFERENTIATION ; PLURIPOTENCY ; DELIVERY
英文摘要:

The bone defect repairing is still a challenge in orthopedics. As the gene engineering bones have been used in the bone repairing clinic, the scaffold construction is a critical fact to be considered. This study aims to construct optimal scaffolds using adipose tissue in the bone repair together with the gene engineering osteocytes. Rat adipose stem cells (ASC) were prepared; the cells were transduced with the OCT-4 gene carrying lentiviral vectors (OCT-4-Lv). Artificial bone defects were created in the rat femoral bone. The bone defects were filled up with adipose scaffolds and shaped by using surrounding muscles and supported with orthopedic splints. ASCs with or without transducing the OCT-4-Lv were injected into the adipose scaffolds. The rats were sacrificed 12 weeks after the surgery. After receiving the OCT-4-Lv, the expressions of OCT-4, RUNX2 and osteocalcin were detected in the ASCs. X-ray examination showed that rats received the OCT-4-Lv transduced ASCs together with the adipose pad had new bone formation in the defect area; none of the control rats showed any new bone formation in situ. The results were supported by histological assessment. Using adipose scaffold and OCT-4-modified ASC transplantation can repair bone defects.

语种: 英语
所属项目编号: 81171692
项目资助者: China National Natural Science Foundation
WOS记录号: WOS:000327510800003
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/52920
Appears in Collections:北京大学口腔医学院_期刊论文

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作者单位: 1.Harbin Med Univ, Affiliated Hosp 1, Dept Orthopaed, Harbin 150001, Heilongjiang, Peoples R China
2.Peking Univ, Sch Stomatol, Dept Cent Lab, Beijing 100081, Peoples R China

Recommended Citation:
Li, Weiming,Fan, Jingzhang,Chen, Feng,et al. Construction of adipose scaffold for bone repair with gene engineering bone cells[J]. EXPERIMENTAL BIOLOGY AND MEDICINE,2013,238(12):1350-1354.
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