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学科主题: 临床医学
题名:
Simultaneous determination of liensinine, isoliensinine and neferine from seed embryo of Nelumbo nucifera Gaertn. in rat plasma by a rapid HPLC method and its application to a pharmacokinetic study
作者: Huang, Ying2; Zhao, Libo3; Bai, Yun1; Liu, Ping1; Wang, Jialing1; Xiang, Jizhou1
关键词: HPLC ; Isoliensinine ; Liensinine ; Neferine ; Pharmacokinetics
刊名: ARZNEIMITTEL-FORSCHUNG-DRUG RESEARCH
发表日期: 2011
卷: 61, 期:6, 页:347-352
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Chemistry, Medicinal ; Chemistry, Multidisciplinary ; Pharmacology & Pharmacy
研究领域[WOS]: Pharmacology & Pharmacy ; Chemistry
关键词[WOS]: ALKALOIDS ; LOTI ; CHROMATOGRAPHY ; SEPARATION
英文摘要:

A rapid and specific HPLC method has been developed and validated for the simultaneous determination of liensinine (CAS 2586-96-1), isoliensinine (CAS 6817-41-0) and neferine (CAS 2292-16-2) in rat plasma. The sample was prepared by a liquid-liquid extraction with diethyl ether and the recovery was above 80 % from the plasma for the three compounds. Chromatographic separation was achieved with a Hypersil BDS C(18) column (4.0 mm x 250 mm, particle size 5 pm). A mobile phase consisting of methanol : 0.2 M KH(2)PO(4) : 0.2 M NaOH : triethylamine (71 : 17 : 12 : 0.002, v/v/v/v, pH 9.2-9.3) was slowly delivered at 0.8 ml/min in isocratic mode with a detection wavelength of 282 nm. The linearity of calibration curves were good (r > 0.999) in the concentration range of 0.031-2.00 mu g/ml. The lower limit of quantification can reach 0.03 mu g/ml for the three compounds. The intra-day and inter-day variations estimated with QC samples were less than 8% for the three tested concentration levels. This developed method was applied in the plasma pharmacokinetic study of total bisbenzylisoquinoline alkaloids (TAL) of the lotus flower (Lian Zi Xin) following a single oral and intravenous administration of TAL in rats.

语种: 英语
WOS记录号: WOS:000292536500004
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/52923
Appears in Collections:北京大学第二临床医学院_药剂科_期刊论文

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作者单位: 1.WuXi AppTec Co Ltd, Wuxi, Peoples R China
2.Huazhong Univ Sci & Technol, Dept Pharmacol, Tongji Med Coll, Wuhan 430030, Hubei, Peoples R China
3.Peking Univ, Dept Pharm, Peoples Hosp, Beijing 100871, Peoples R China

Recommended Citation:
Huang, Ying,Zhao, Libo,Bai, Yun,et al. Simultaneous determination of liensinine, isoliensinine and neferine from seed embryo of Nelumbo nucifera Gaertn. in rat plasma by a rapid HPLC method and its application to a pharmacokinetic study[J]. ARZNEIMITTEL-FORSCHUNG-DRUG RESEARCH,2011,61(6):347-352.
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