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学科主题临床医学
Simultaneous determination of liensinine, isoliensinine and neferine from seed embryo of Nelumbo nucifera Gaertn. in rat plasma by a rapid HPLC method and its application to a pharmacokinetic study
Huang, Ying2; Zhao, Libo3; Bai, Yun1; Liu, Ping1; Wang, Jialing1; Xiang, Jizhou1
关键词Hplc Isoliensinine Liensinine Neferine Pharmacokinetics
刊名ARZNEIMITTEL-FORSCHUNG-DRUG RESEARCH
2011
61期:6页:347-352
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Chemistry, Medicinal ; Chemistry, Multidisciplinary ; Pharmacology & Pharmacy
研究领域[WOS]Pharmacology & Pharmacy ; Chemistry
关键词[WOS]ALKALOIDS ; LOTI ; CHROMATOGRAPHY ; SEPARATION
英文摘要

A rapid and specific HPLC method has been developed and validated for the simultaneous determination of liensinine (CAS 2586-96-1), isoliensinine (CAS 6817-41-0) and neferine (CAS 2292-16-2) in rat plasma. The sample was prepared by a liquid-liquid extraction with diethyl ether and the recovery was above 80 % from the plasma for the three compounds. Chromatographic separation was achieved with a Hypersil BDS C(18) column (4.0 mm x 250 mm, particle size 5 pm). A mobile phase consisting of methanol : 0.2 M KH(2)PO(4) : 0.2 M NaOH : triethylamine (71 : 17 : 12 : 0.002, v/v/v/v, pH 9.2-9.3) was slowly delivered at 0.8 ml/min in isocratic mode with a detection wavelength of 282 nm. The linearity of calibration curves were good (r > 0.999) in the concentration range of 0.031-2.00 mu g/ml. The lower limit of quantification can reach 0.03 mu g/ml for the three compounds. The intra-day and inter-day variations estimated with QC samples were less than 8% for the three tested concentration levels. This developed method was applied in the plasma pharmacokinetic study of total bisbenzylisoquinoline alkaloids (TAL) of the lotus flower (Lian Zi Xin) following a single oral and intravenous administration of TAL in rats.

语种英语
WOS记录号WOS:000292536500004
引用统计
被引频次:9[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/52923
专题北京大学第二临床医学院_药剂科
作者单位1.WuXi AppTec Co Ltd, Wuxi, Peoples R China
2.Huazhong Univ Sci & Technol, Dept Pharmacol, Tongji Med Coll, Wuhan 430030, Hubei, Peoples R China
3.Peking Univ, Dept Pharm, Peoples Hosp, Beijing 100871, Peoples R China
推荐引用方式
GB/T 7714
Huang, Ying,Zhao, Libo,Bai, Yun,et al. Simultaneous determination of liensinine, isoliensinine and neferine from seed embryo of Nelumbo nucifera Gaertn. in rat plasma by a rapid HPLC method and its application to a pharmacokinetic study[J]. ARZNEIMITTEL-FORSCHUNG-DRUG RESEARCH,2011,61(6):347-352.
APA Huang, Ying,Zhao, Libo,Bai, Yun,Liu, Ping,Wang, Jialing,&Xiang, Jizhou.(2011).Simultaneous determination of liensinine, isoliensinine and neferine from seed embryo of Nelumbo nucifera Gaertn. in rat plasma by a rapid HPLC method and its application to a pharmacokinetic study.ARZNEIMITTEL-FORSCHUNG-DRUG RESEARCH,61(6),347-352.
MLA Huang, Ying,et al."Simultaneous determination of liensinine, isoliensinine and neferine from seed embryo of Nelumbo nucifera Gaertn. in rat plasma by a rapid HPLC method and its application to a pharmacokinetic study".ARZNEIMITTEL-FORSCHUNG-DRUG RESEARCH 61.6(2011):347-352.
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