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学科主题: 药学
题名:
Permeation of vanadium(III, IV, V)-dipicolinate complexes across MDCK cell monolayer and comparison with Caco-2 cells
作者: Zhang, Y1; Yang, XD1; Wang, K1
关键词: vanadium complex ; MDCK ; Caco-2 ; intestinal absorption ; diabetes
刊名: CHINESE SCIENCE BULLETIN
发表日期: 2005-09-01
DOI: 10.1360/982005-102
卷: 50, 期:17, 页:1854-1859
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
关键词[WOS]: BIS(MALTOLATO)OXOVANADIUM(IV) ; METABOLISM ; ABSORPTION
英文摘要:

The permeation and cytotoxicity of three insulin-mimetic vanadium(III, IV, V)-dipicolinate complexes were studied using the MDCK cell monolayer in comparison with the Caco-2 cells. On MDCK cell monolayer, the apparent permeation coefficients (P-app) were estimated to be (7.5 1.0) X 10(-6), (1.0 +/- 0.2) X 10(-6), (1.7 +/- 0.4) X 10(-6) cm/s for V(V), V(IV), and V(III)-dipic complexes, respectively. The permeability of V(V)-dipic complexes is much better than the others, which is in agreement with its better hypoglycemic effect in animal tests. On Caco-2 cell monolayer, P-app were found to be in the range of 1 - 3 X le cm/s and not to be affected by excessive amounts of dipicolinate ligand. By contrast, the permeability in the AP -> BL direction across the MDCK monolayer increased greatly in the presence of free ligands, suggesting existence of active transport mechanism of vanadium complex anions on the MDCK cells. The cytotoxicity of the three complexes was found similar and the IC50 were measured in the range of 0.6-0.9 mmol/L for MDCK cells and 1.6-2 mmol/L for Caco-2 cells. The cytotoxicity of three vanadium complexes was conceivably in consistence with their permeability, suggesting that the toxicity, permeation and cellular metabolism of vanadium complexes are closely related.

语种: 英语
WOS记录号: WOS:000233072700009
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/52927
Appears in Collections:北京大学药学院_化学生物学系_期刊论文

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作者单位: 1.Peking Univ, Sch Pharmaceut Sci, Lab Prevent Pharmaceut, Dept Biol Chem, Beijing 100083, Peoples R China
2.Peking Univ, Sch Pharmaceut Sci, Natl Lab Nat & Biomimet Drugs, Beijing 100083, Peoples R China

Recommended Citation:
Zhang, Y,Yang, XD,Wang, K. Permeation of vanadium(III, IV, V)-dipicolinate complexes across MDCK cell monolayer and comparison with Caco-2 cells[J]. CHINESE SCIENCE BULLETIN,2005,50(17):1854-1859.
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