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Permeation of vanadium(III, IV, V)-dipicolinate complexes across MDCK cell monolayer and comparison with Caco-2 cells
Zhang, Y1; Yang, XD1; Wang, K1
关键词Vanadium Complex Mdck Caco-2 Intestinal Absorption Diabetes
刊名CHINESE SCIENCE BULLETIN
2005-09-01
DOI10.1360/982005-102
50期:17页:1854-1859
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Multidisciplinary Sciences
研究领域[WOS]Science & Technology - Other Topics
关键词[WOS]BIS(MALTOLATO)OXOVANADIUM(IV) ; METABOLISM ; ABSORPTION
英文摘要

The permeation and cytotoxicity of three insulin-mimetic vanadium(III, IV, V)-dipicolinate complexes were studied using the MDCK cell monolayer in comparison with the Caco-2 cells. On MDCK cell monolayer, the apparent permeation coefficients (P-app) were estimated to be (7.5 1.0) X 10(-6), (1.0 +/- 0.2) X 10(-6), (1.7 +/- 0.4) X 10(-6) cm/s for V(V), V(IV), and V(III)-dipic complexes, respectively. The permeability of V(V)-dipic complexes is much better than the others, which is in agreement with its better hypoglycemic effect in animal tests. On Caco-2 cell monolayer, P-app were found to be in the range of 1 - 3 X le cm/s and not to be affected by excessive amounts of dipicolinate ligand. By contrast, the permeability in the AP -> BL direction across the MDCK monolayer increased greatly in the presence of free ligands, suggesting existence of active transport mechanism of vanadium complex anions on the MDCK cells. The cytotoxicity of the three complexes was found similar and the IC50 were measured in the range of 0.6-0.9 mmol/L for MDCK cells and 1.6-2 mmol/L for Caco-2 cells. The cytotoxicity of three vanadium complexes was conceivably in consistence with their permeability, suggesting that the toxicity, permeation and cellular metabolism of vanadium complexes are closely related.

语种英语
WOS记录号WOS:000233072700009
引用统计
被引频次:4[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/52927
专题北京大学药学院_化学生物学系
作者单位1.Peking Univ, Sch Pharmaceut Sci, Lab Prevent Pharmaceut, Dept Biol Chem, Beijing 100083, Peoples R China
2.Peking Univ, Sch Pharmaceut Sci, Natl Lab Nat & Biomimet Drugs, Beijing 100083, Peoples R China
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GB/T 7714
Zhang, Y,Yang, XD,Wang, K. Permeation of vanadium(III, IV, V)-dipicolinate complexes across MDCK cell monolayer and comparison with Caco-2 cells[J]. CHINESE SCIENCE BULLETIN,2005,50(17):1854-1859.
APA Zhang, Y,Yang, XD,&Wang, K.(2005).Permeation of vanadium(III, IV, V)-dipicolinate complexes across MDCK cell monolayer and comparison with Caco-2 cells.CHINESE SCIENCE BULLETIN,50(17),1854-1859.
MLA Zhang, Y,et al."Permeation of vanadium(III, IV, V)-dipicolinate complexes across MDCK cell monolayer and comparison with Caco-2 cells".CHINESE SCIENCE BULLETIN 50.17(2005):1854-1859.
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