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学科主题: 临床医学
题名:
MicroRNA array and microarray evaluation of endometrial receptivity in patients with high serum progesterone levels on the day of hCG administration
作者: Li, Rong1; Qiao, Jie1; Wang, Lina1; Li, Li1; Zhen, Xiumei1; Liu, Ping1; Zheng, Xiaoying2
刊名: REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY
发表日期: 2011-03-06
DOI: 10.1186/1477-7827-9-29
卷: 9
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Endocrinology & Metabolism ; Reproductive Biology
研究领域[WOS]: Endocrinology & Metabolism ; Reproductive Biology
关键词[WOS]: UTERINE RECEPTIVITY ; PREMATURE LUTEINIZATION ; EMBRYO IMPLANTATION ; MENSTRUAL-CYCLE ; GONADOTROPIN ; EXPRESSION ; DONATION ; MODEL
英文摘要:

Background: To determine the effect of higher progesterone (P) level on endometrial receptivity.

Methods: This was a prospective analysis conducted in the Reproductive Medical Center of Peking University Third Hospital. All patients received IVF treatment and canceled embryo transfer in the same cycle and were divided into group 1 (normal P; 7 patients) and group 2 (elevated P; 12 patients). Endometrial biopsies were performed 6 days after oocyte retrieval. The global miRNA and mRNA gene expressions in endometrial biopsies were investigated with a V4.0 miRNA probe and 22 K Human Genome Array. Fold ratios were derived to compare gene regulation between the groups. Spp1 and Ang gene expression was selected to verify the array results by RT-PCR and the protein expression of osteopontin and VEGF was determined using an immunohistochemical method.

Results: There were 4 miRNA (all down-regulated) and 22 mRNA (13 up-regulated and 9 down-regulated) exhibiting differential expression between the groups on the microRNA and microarray chips. miRNA-451, Spp1, and Ang expression in RT-PCR verified the array results. Osteopontin and VEGF were also shown to have positive expression in the endometrium.

Conclusions: Data from microRNA and microarray analysis suggests dissimilar endometrial receptivity in patients with high P levels on the day of hCG, and elevated osteopontin and decreased VEGF had poor pregnancy rates.

语种: 英语
所属项目编号: 2007BAI04B04 ; 2007CB511901
项目资助者: Mega-projects of Science Research for the 11th Five-year Plan ; National Basic Research Program of Chinam, 973 Program
WOS记录号: WOS:000289002900001
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/52938
Appears in Collections:北京大学第三临床医学院_期刊论文

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作者单位: 1.Peking Univ, Hosp 3, Beijing 100191, Peoples R China
2.Peking Univ, Inst Populat Res, Beijing 10081, Peoples R China

Recommended Citation:
Li, Rong,Qiao, Jie,Wang, Lina,et al. MicroRNA array and microarray evaluation of endometrial receptivity in patients with high serum progesterone levels on the day of hCG administration[J]. REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY,2011,9.
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