IR@PKUHSC  > 北京大学第三临床医学院
学科主题临床医学
MicroRNA array and microarray evaluation of endometrial receptivity in patients with high serum progesterone levels on the day of hCG administration
Li, Rong1; Qiao, Jie1; Wang, Lina1; Li, Li1; Zhen, Xiumei1; Liu, Ping1; Zheng, Xiaoying2
刊名REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY
2011-03-06
DOI10.1186/1477-7827-9-29
9
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Endocrinology & Metabolism ; Reproductive Biology
研究领域[WOS]Endocrinology & Metabolism ; Reproductive Biology
关键词[WOS]UTERINE RECEPTIVITY ; PREMATURE LUTEINIZATION ; EMBRYO IMPLANTATION ; MENSTRUAL-CYCLE ; GONADOTROPIN ; EXPRESSION ; DONATION ; MODEL
英文摘要

Background: To determine the effect of higher progesterone (P) level on endometrial receptivity.

Methods: This was a prospective analysis conducted in the Reproductive Medical Center of Peking University Third Hospital. All patients received IVF treatment and canceled embryo transfer in the same cycle and were divided into group 1 (normal P; 7 patients) and group 2 (elevated P; 12 patients). Endometrial biopsies were performed 6 days after oocyte retrieval. The global miRNA and mRNA gene expressions in endometrial biopsies were investigated with a V4.0 miRNA probe and 22 K Human Genome Array. Fold ratios were derived to compare gene regulation between the groups. Spp1 and Ang gene expression was selected to verify the array results by RT-PCR and the protein expression of osteopontin and VEGF was determined using an immunohistochemical method.

Results: There were 4 miRNA (all down-regulated) and 22 mRNA (13 up-regulated and 9 down-regulated) exhibiting differential expression between the groups on the microRNA and microarray chips. miRNA-451, Spp1, and Ang expression in RT-PCR verified the array results. Osteopontin and VEGF were also shown to have positive expression in the endometrium.

Conclusions: Data from microRNA and microarray analysis suggests dissimilar endometrial receptivity in patients with high P levels on the day of hCG, and elevated osteopontin and decreased VEGF had poor pregnancy rates.

语种英语
WOS记录号WOS:000289002900001
项目编号2007BAI04B04 ; 2007CB511901
资助机构Mega-projects of Science Research for the 11th Five-year Plan ; National Basic Research Program of Chinam, 973 Program
引用统计
被引频次:37[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/52938
专题北京大学第三临床医学院
北京大学第三临床医学院_生殖医学中心
作者单位1.Peking Univ, Hosp 3, Beijing 100191, Peoples R China
2.Peking Univ, Inst Populat Res, Beijing 10081, Peoples R China
推荐引用方式
GB/T 7714
Li, Rong,Qiao, Jie,Wang, Lina,et al. MicroRNA array and microarray evaluation of endometrial receptivity in patients with high serum progesterone levels on the day of hCG administration[J]. REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY,2011,9.
APA Li, Rong.,Qiao, Jie.,Wang, Lina.,Li, Li.,Zhen, Xiumei.,...&Zheng, Xiaoying.(2011).MicroRNA array and microarray evaluation of endometrial receptivity in patients with high serum progesterone levels on the day of hCG administration.REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY,9.
MLA Li, Rong,et al."MicroRNA array and microarray evaluation of endometrial receptivity in patients with high serum progesterone levels on the day of hCG administration".REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY 9(2011).
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Li, Rong]的文章
[Qiao, Jie]的文章
[Wang, Lina]的文章
百度学术
百度学术中相似的文章
[Li, Rong]的文章
[Qiao, Jie]的文章
[Wang, Lina]的文章
必应学术
必应学术中相似的文章
[Li, Rong]的文章
[Qiao, Jie]的文章
[Wang, Lina]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。