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Pyruvate-Associated Acid Resistance in Bacteria
Wu, Jianting1; Li, Yannan3; Cai, Zhiming1,2; Jin, Ye4,5
刊名APPLIED AND ENVIRONMENTAL MICROBIOLOGY
2014-07-01
DOI10.1128/AEM.01001-14
80期:14页:4108-4113
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biotechnology & Applied Microbiology ; Microbiology
研究领域[WOS]Biotechnology & Applied Microbiology ; Microbiology
关键词[WOS]ENTEROHEMORRHAGIC ESCHERICHIA-COLI ; GENE-EXPRESSION ; CYCLIC-AMP ; TRANSCRIPTION ACTIVATION ; STATIONARY-PHASE ; RECEPTOR PROTEIN ; METABOLIC FLUXES ; ACETATE ; GROWTH ; PH
英文摘要

Glucose confers acid resistance on exponentially growing bacteria by repressing formation of the cyclic AMP (cAMP)-cAMP receptor protein (CRP) complex and consequently activating acid resistance genes. Therefore, in a glucose-rich growth environment, bacteria are capable of resisting acidic stresses due to low levels of cAMP-CRP. Here we reveal a second mechanism for glucose-conferred acid resistance. We show that glucose induces acid resistance in exponentially growing bacteria through pyruvate, the glycolysis product. Pyruvate and/or the downstream metabolites induce expression of the small noncoding RNA (sncRNA) Spot42, and the sncRNA, in turn, activates expression of the master regulator of acid resistance, RpoS. In contrast to glucose, pyruvate has little effect on levels of the cAMP-CRP complex and does not require the complex for its effects on acid resistance. Another important difference between glucose and pyruvate is that pyruvate can be produced by bacteria. This means that bacteria have the potential to protect themselves from acidic stresses by controlling glucose-derived generation of pyruvate, pyruvate-acetate efflux, or reversion from acetate to pyruvate. We tested this possibility by shutting down pyruvate-acetate efflux and found that the resulting accumulation of pyruvate elevated acid resistance. Many sugars can be broken into glucose, and the subsequent glycolysis generates pyruvate. Therefore, pyruvate-associated acid resistance is not confined to glucose-grown bacteria but is functional in bacteria grown on various sugars.

语种英语
WOS记录号WOS:000338706800004
项目编号C010301 ; JCYJ20120614155650545 ; 2014CB745200
资助机构National Natural Science Foundation of China ; Basic Research Program of Shenzhen ; National Program on Key Basic Research Project
引用统计
被引频次:8[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/52940
专题北京大学深圳医院
作者单位1.Peking Univ, Shenzhen Hosp, Guangdong & Shenzhen Key Lab Male Reprod Med & Ge, Shenzhen, Peoples R China
2.Shenzhen Univ, Affiliated Hosp 1, Shenzhen Peoples Hosp 2, Shenzhen Key Lab Genitourinary Tumor, Shenzhen, Peoples R China
3.Shenzhen Univ Town, Shenzhen Inst Adv Technol, Inst Biomed & Biotechnol, Ctr Synthet Biol Engn Res, Shenzhen, Peoples R China
4.Chinese Univ Hong Kong, Dept Med & Therapeut, Shatin, Hong Kong, Peoples R China
5.Chinese Univ Hong Kong, State Key Lab Digest Dis, Shatin, Hong Kong, Peoples R China
推荐引用方式
GB/T 7714
Wu, Jianting,Li, Yannan,Cai, Zhiming,et al. Pyruvate-Associated Acid Resistance in Bacteria[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY,2014,80(14):4108-4113.
APA Wu, Jianting,Li, Yannan,Cai, Zhiming,&Jin, Ye.(2014).Pyruvate-Associated Acid Resistance in Bacteria.APPLIED AND ENVIRONMENTAL MICROBIOLOGY,80(14),4108-4113.
MLA Wu, Jianting,et al."Pyruvate-Associated Acid Resistance in Bacteria".APPLIED AND ENVIRONMENTAL MICROBIOLOGY 80.14(2014):4108-4113.
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