学科主题临床医学
Senescence marker protein 30 in acute liver failure: validation of a mass spectrometry proteomics assay
Lv, Sa; Wang, Jiang-Hua; Liu, Feng; Gao, Yan; Fei, Ran; Du, Shao-Cai; Wei, Lai
刊名BMC GASTROENTEROLOGY
2008-05-28
DOI10.1186/1471-230X-8-17
8
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Gastroenterology & Hepatology
研究领域[WOS]Gastroenterology & Hepatology
关键词[WOS]HEPATOMA H4-II-E CELLS ; DEOXYRIBONUCLEIC-ACID SYNTHESIS ; REGENERATING RAT-LIVER ; HEP G2 CELLS ; ENDOGENOUS REGUCALCIN ; CARBON-TETRACHLORIDE ; SMP30 ; PROLIFERATION ; SERUM ; OVEREXPRESSION
英文摘要

Background: Our previous proteomic study showed that the senescence marker protein (SMP30) is selectively present in the plasma of a murine model of acute liver failure (ALF). The aim of this study was to validate this SMP30 expression in the plasma and liver tissues of mice and humans with ALF.

Methods: After the proteomic analysis of plasma from a murine model of D-galactosamine/ lipopolysaccharide (GalN/LPS)-induced ALF by two-dimensional electrophoresis (2-DE) and mass spectrometry, the expression levels of SMP30 in the plasma and liver tissues were validated by western blot and RT-PCR analyses. These results were then confirmed in plasma samples from humans.

Results: These data validate the results of 2-DE, and western blot showed that SMP30 protein levels were only elevated in the plasma of ALF mice. Further analysis revealed that GalN/LPS induced the downregulation of SMP30 protein levels in liver tissues (by approximately 25% and 16% in the GalN/LPS-treated mice and in the treated mice that survived, respectively; P < 0.01). Hepatic SMP30 mRNA levels decreased by about 90% only in the mice that survived the GalN/LPS treatment. Importantly, plasma obtained from patients with ALF also contained higher levels of SMP30, about (3.65 +/- 0.34) times those observed in healthy volunteers.

Conclusion: This study shows that SMP30 is not only a potential biomarker for the diagnosis and even prognosis of ALF. It also plays a very important role in a self-protective mechanism in survival and participates in the pathophysiological processes of ALF.

语种英语
WOS记录号WOS:000257344800001
引用统计
被引频次:12[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/52947
专题北京大学第二临床医学院_北京大学肝病研究所
作者单位Peking Univ, Inst Hepatol, Peking Univ Peoples Hosp, Beijing 100044, Peoples R China
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GB/T 7714
Lv, Sa,Wang, Jiang-Hua,Liu, Feng,et al. Senescence marker protein 30 in acute liver failure: validation of a mass spectrometry proteomics assay[J]. BMC GASTROENTEROLOGY,2008,8.
APA Lv, Sa.,Wang, Jiang-Hua.,Liu, Feng.,Gao, Yan.,Fei, Ran.,...&Wei, Lai.(2008).Senescence marker protein 30 in acute liver failure: validation of a mass spectrometry proteomics assay.BMC GASTROENTEROLOGY,8.
MLA Lv, Sa,et al."Senescence marker protein 30 in acute liver failure: validation of a mass spectrometry proteomics assay".BMC GASTROENTEROLOGY 8(2008).
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