|Identification and expression analysis of novel LAGE-1 alleles with single nucleotide polymorphisms in cancer patients|
|Shao, Yi1,2,3; Sun, Zhen-yuan1,2,3; Sun, Shi-wei4; Zhao, Yi4; Sin, Wan Yee5; Yuan, Yan-hua3; Simpson, Andrew J.6; Old, Lloyd J.6; Sang, Xin-ting1,2; Mao, Yi-lei1,2; Xie, Yong5; Huang, Jie-fu1,2; Zhao, Hai-tao1,2|
|关键词||Single Nucleotide Polymorphism Lage-1 Gene Allele Transcript Variant Cancer|
|刊名||JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY|
|WOS标题词||Science & Technology|
|关键词[WOS]||T-CELL RESPONSES ; HUMAN TUMOR-ANTIGENS ; HEPATOCELLULAR-CARCINOMA ; ANTIBODY-RESPONSES ; IMMUNE-RESPONSE ; LUNG-CANCER ; NY-ESO-1 ; PEPTIDE ; GENE ; LYMPHOCYTES|
Objective The purpose of this study is to identify single nucleotide polymorphisms (SNPs) in the coding region alleles of the X chromosomal LAGE-1 gene, and investigate the frequency of such SNPs in both cancer patients and healthy controls, and thus determine the potential significance of these SNPs with respect to cancer vaccine therapy.
Methods In this study, different mRNAs transcribed from the LAGE-1 gene were identified by RT-PCR from healthy donors and cancer patients samples.
Results A new LAGE-1 allele containing three coding region SNPs (69A/G, 317C/G, and 397T/G) were identified. The allele is highly expressed as the LAGE-1a mRNA variant AY679089 in some of the cancer patients. The three SNPs altered the LAGE-1 gene sequence to that of NYESO-1 at both the nucleotide and amino acid level.
Conclusion There is a high frequency of the LAGE-1 gene allele with SNPs in coding regions in cancer patients. There was a clear relationship between the variant AY679089 and gastric cancer. The SNPs may lead to accelerated progress of poorly differentiated gastric cancer. The SNPs found in these alleles may also alter the immunological characteristics of LAGE-1a and should be taken into account if this antigen is adopted as a cancer vaccine component.
|作者单位||1.Chinese Acad Med Sci, Peking Union Med Coll Hosp, Dept Liver Surg, Beijing 100730, Peoples R China|
2.PUMC, Beijing 100730, Peoples R China
3.Peking Univ, Sch Oncol, Beijing Canc Hosp, Canc Immunol Therapy & Diagnosis Ctr, Beijing 100871, Peoples R China
4.Chinese Acad Sci, Inst Comp Technol, Bioinformat Lab, Beijing, Peoples R China
5.Hong Kong Univ Sci & Technol, Dept Biol, Kowloon, Hong Kong, Peoples R China
6.Ludwig Inst Canc Res, Mem Sloan Kettering Canc Ctr, New York Branch, New York, NY USA
|Shao, Yi,Sun, Zhen-yuan,Sun, Shi-wei,et al. Identification and expression analysis of novel LAGE-1 alleles with single nucleotide polymorphisms in cancer patients[J]. JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY,2008,134(4):495-502.|
|APA||Shao, Yi.,Sun, Zhen-yuan.,Sun, Shi-wei.,Zhao, Yi.,Sin, Wan Yee.,...&Zhao, Hai-tao.(2008).Identification and expression analysis of novel LAGE-1 alleles with single nucleotide polymorphisms in cancer patients.JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY,134(4),495-502.|
|MLA||Shao, Yi,et al."Identification and expression analysis of novel LAGE-1 alleles with single nucleotide polymorphisms in cancer patients".JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY 134.4(2008):495-502.|