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学科主题: 临床医学
题名:
The lamin-A/C-LAP2 alpha-BAF1 protein complex regulates mitotic spindle assembly and positioning
作者: Qi, Ran1,2; Xu, Nan1,2; Wang, Gang1,2; Ren, He1,2; Li, Si1,2; Lei, Jun1,2; Lin, Qiaoyu1,2; Wang, Lihao1,2; Gu, Xin1,2; Zhang, Hongyin3; Jiang, Qing1,2; Zhang, Chuanmao1,2
关键词: BAF1 ; LAP2 ; Cell cycle ; Lamin-A/C ; Spindle assembly ; Spindle positioning
刊名: JOURNAL OF CELL SCIENCE
发表日期: 2015-08-01
DOI: 10.1242/jcs.164566
卷: 128, 期:15, 页:2830-2841
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Cell Biology
研究领域[WOS]: Cell Biology
关键词[WOS]: TO-AUTOINTEGRATION FACTOR ; NUCLEAR-ENVELOPE ; GENE-EXPRESSION ; PULLING FORCES ; MOUSE OOCYTES ; CELL-DIVISION ; LAMINA ; ACTIN ; DISEASE ; ORIENTATION
英文摘要:

Some nuclear proteins that are crucial in interphase relocate during the G2/M-phase transition in order to perform their mitotic functions. However, how they perform these functions and the underlying mechanisms remain largely unknown. Here, we report that a fraction of the nuclear periphery proteins lamin-A/C, LAP2 alpha and BAF1 (also known as BANF1) relocate to the spindle and the cell cortex in mitosis. Knockdown of these proteins by using RNA interference (RNAi) induces short and fluffy spindle formation, and disconnection of the spindle from the cell cortex. Disrupting the microtubule assembly leads to accumulation of these proteins in the cell cortex, whereas depolymerizing the actin microfilaments results in the formation of short spindles. We further demonstrate that these proteins are part of a stable complex that links the mitotic spindle to the cell cortex and the spindle matrix by binding to spindle-associated dynein, the actin filaments in the cell cortex and the spindle matrix. Taken together, our findings unveil a unique mechanism where the nuclear periphery proteins lamin-A/C, LAP2 alpha and BAF1 are assembled into a protein complex during mitosis in order to regulate assembly and positioning of the mitotic spindle.

语种: 英语
所属项目编号: 91313302 ; 31030044 ; 31371365 ; 2010CB833705 ; 2014CB138402
项目资助者: National Natural Science Foundation of China (NSFC) ; State Key Basic Research and Development Plan of Ministry of Science and Technology of China
WOS记录号: WOS:000359782100010
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/52954
Appears in Collections:北京大学临床肿瘤学院_期刊论文

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作者单位: 1.Minist Educ, Key Lab Cell Proliferat & Differentiat, Beijing 100871, Peoples R China
2.Coll Life Sci, State Key Lab Membrane Biol, Beijing 100871, Peoples R China
3.Peking Univ, Canc Res Ctr, Peking Univ Hosp, Beijing 100871, Peoples R China

Recommended Citation:
Qi, Ran,Xu, Nan,Wang, Gang,et al. The lamin-A/C-LAP2 alpha-BAF1 protein complex regulates mitotic spindle assembly and positioning[J]. JOURNAL OF CELL SCIENCE,2015,128(15):2830-2841.
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