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学科主题临床医学
The lamin-A/C-LAP2 alpha-BAF1 protein complex regulates mitotic spindle assembly and positioning
Qi, Ran1,2; Xu, Nan1,2; Wang, Gang1,2; Ren, He1,2; Li, Si1,2; Lei, Jun1,2; Lin, Qiaoyu1,2; Wang, Lihao1,2; Gu, Xin1,2; Zhang, Hongyin3; Jiang, Qing1,2; Zhang, Chuanmao1,2
关键词Baf1 Lap2 Cell Cycle Lamin-a/c Spindle Assembly Spindle Positioning
刊名JOURNAL OF CELL SCIENCE
2015-08-01
DOI10.1242/jcs.164566
128期:15页:2830-2841
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cell Biology
研究领域[WOS]Cell Biology
关键词[WOS]TO-AUTOINTEGRATION FACTOR ; NUCLEAR-ENVELOPE ; GENE-EXPRESSION ; PULLING FORCES ; MOUSE OOCYTES ; CELL-DIVISION ; LAMINA ; ACTIN ; DISEASE ; ORIENTATION
英文摘要

Some nuclear proteins that are crucial in interphase relocate during the G2/M-phase transition in order to perform their mitotic functions. However, how they perform these functions and the underlying mechanisms remain largely unknown. Here, we report that a fraction of the nuclear periphery proteins lamin-A/C, LAP2 alpha and BAF1 (also known as BANF1) relocate to the spindle and the cell cortex in mitosis. Knockdown of these proteins by using RNA interference (RNAi) induces short and fluffy spindle formation, and disconnection of the spindle from the cell cortex. Disrupting the microtubule assembly leads to accumulation of these proteins in the cell cortex, whereas depolymerizing the actin microfilaments results in the formation of short spindles. We further demonstrate that these proteins are part of a stable complex that links the mitotic spindle to the cell cortex and the spindle matrix by binding to spindle-associated dynein, the actin filaments in the cell cortex and the spindle matrix. Taken together, our findings unveil a unique mechanism where the nuclear periphery proteins lamin-A/C, LAP2 alpha and BAF1 are assembled into a protein complex during mitosis in order to regulate assembly and positioning of the mitotic spindle.

语种英语
WOS记录号WOS:000359782100010
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被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/52954
专题北京大学临床肿瘤学院
作者单位1.Minist Educ, Key Lab Cell Proliferat & Differentiat, Beijing 100871, Peoples R China
2.Coll Life Sci, State Key Lab Membrane Biol, Beijing 100871, Peoples R China
3.Peking Univ, Canc Res Ctr, Peking Univ Hosp, Beijing 100871, Peoples R China
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GB/T 7714
Qi, Ran,Xu, Nan,Wang, Gang,et al. The lamin-A/C-LAP2 alpha-BAF1 protein complex regulates mitotic spindle assembly and positioning[J]. JOURNAL OF CELL SCIENCE,2015,128(15):2830-2841.
APA Qi, Ran.,Xu, Nan.,Wang, Gang.,Ren, He.,Li, Si.,...&Zhang, Chuanmao.(2015).The lamin-A/C-LAP2 alpha-BAF1 protein complex regulates mitotic spindle assembly and positioning.JOURNAL OF CELL SCIENCE,128(15),2830-2841.
MLA Qi, Ran,et al."The lamin-A/C-LAP2 alpha-BAF1 protein complex regulates mitotic spindle assembly and positioning".JOURNAL OF CELL SCIENCE 128.15(2015):2830-2841.
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