北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学第二临床医学院  > 妇科肿瘤中心  > 期刊论文
学科主题: 临床医学
题名:
Identification of Biomarkers for Endometriosis Using Clinical Proteomics
作者: Zhao, Yang1; Liu, Ya-Nan1; Li, Yi1; Tian, Li2; Ye, Xue1; Cui, Heng1; Chang, Xiao-Hong1
关键词: ClinProt ; Endometriosis ; Liquid Chromatography-tandem Mass Spectrometry ; Magnetic Bead ; Matrix-assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry ; Proteomics
刊名: CHINESE MEDICAL JOURNAL
发表日期: 2015-02-20
DOI: 10.4103/0366-6999.151108
卷: 128, 期:4, 页:520-527
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medicine, General & Internal
研究领域[WOS]: General & Internal Medicine
关键词[WOS]: FLIGHT MASS-SPECTROMETRY ; DISEASE ; BURDEN
英文摘要:

Background: We investigated possible biomarkers for endometriosis (EM) using the ClinProt technique and proteomics methods.

Methods: We enrolled 50 patients with EM, 34 with benign ovarian neoplasms and 40 healthy volunteers in this study. Serum proteomic spectra were generated by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MS) combined with weak cationic exchange (WCX) magnetic beads. Possible biomarkers were analyzed by a random and repeat pattern model-validation method that we designed, and ClinProtools software, results were refined using online liquid chromatography-tandem MS.

Results: We found a cluster of 5 peptides (4210, 5264, 2660, 5635, and 5904 Da), using 3 peptides (4210, 5904, 2660 Da) to discriminate EM patients from healthy volunteers, with 96.67% sensitivity and 100% specificity. We selected 4210 and 5904 m/z, which differed most between patients with EM and controls, and identified them as fragments of ATP1B4, and the fibrinogen alpha (FGA) isoform 1/2 of the FGA chain precursor, respectively.

Conclusions: ClinProt can identify EM biomarkers, which - most notably - distinguish even early-stage or minimal disease. We found 5 stable peaks at 4210, 5264, 2660, 5635, and 5904 Da as potential EM biomarkers, the strongest of which were associated with ATP1B4 (4210 Da) and FGA (5904 Da); this indicates that ATP1B4 and FGA are associated with EM pathogenesis.

语种: 英语
所属项目编号: 30772319 ; 81170544 ; RDB2007-10
项目资助者: National Natural Science Foundation of China ; Peking University People&prime ; s Hospital Research and Development Funds
WOS记录号: WOS:000349523900018
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/52956
Appears in Collections:北京大学第二临床医学院_妇科肿瘤中心_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Peking Univ, Gynecol Oncol Ctr, Peoples Hosp, Beijing 100044, Peoples R China
2.Peking Univ, Ctr Reprod Med, Peoples Hosp, Beijing 100044, Peoples R China

Recommended Citation:
Zhao, Yang,Liu, Ya-Nan,Li, Yi,et al. Identification of Biomarkers for Endometriosis Using Clinical Proteomics[J]. CHINESE MEDICAL JOURNAL,2015,128(4):520-527.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Zhao, Yang]'s Articles
[Liu, Ya-Nan]'s Articles
[Li, Yi]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Zhao, Yang]‘s Articles
[Liu, Ya-Nan]‘s Articles
[Li, Yi]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2018  北京大学医学部 - Feedback
Powered by CSpace