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学科主题临床医学
Identification of Biomarkers for Endometriosis Using Clinical Proteomics
Zhao, Yang1; Liu, Ya-Nan1; Li, Yi1; Tian, Li2; Ye, Xue1; Cui, Heng1; Chang, Xiao-Hong1
关键词Clinprot Endometriosis Liquid Chromatography-tandem Mass Spectrometry Magnetic Bead Matrix-assisted Laser Desorption/ionization Time-of-flight Mass Spectrometry Proteomics
刊名CHINESE MEDICAL JOURNAL
2015-02-20
DOI10.4103/0366-6999.151108
128期:4页:520-527
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Medicine, General & Internal
研究领域[WOS]General & Internal Medicine
关键词[WOS]FLIGHT MASS-SPECTROMETRY ; DISEASE ; BURDEN
英文摘要

Background: We investigated possible biomarkers for endometriosis (EM) using the ClinProt technique and proteomics methods.

Methods: We enrolled 50 patients with EM, 34 with benign ovarian neoplasms and 40 healthy volunteers in this study. Serum proteomic spectra were generated by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MS) combined with weak cationic exchange (WCX) magnetic beads. Possible biomarkers were analyzed by a random and repeat pattern model-validation method that we designed, and ClinProtools software, results were refined using online liquid chromatography-tandem MS.

Results: We found a cluster of 5 peptides (4210, 5264, 2660, 5635, and 5904 Da), using 3 peptides (4210, 5904, 2660 Da) to discriminate EM patients from healthy volunteers, with 96.67% sensitivity and 100% specificity. We selected 4210 and 5904 m/z, which differed most between patients with EM and controls, and identified them as fragments of ATP1B4, and the fibrinogen alpha (FGA) isoform 1/2 of the FGA chain precursor, respectively.

Conclusions: ClinProt can identify EM biomarkers, which - most notably - distinguish even early-stage or minimal disease. We found 5 stable peaks at 4210, 5264, 2660, 5635, and 5904 Da as potential EM biomarkers, the strongest of which were associated with ATP1B4 (4210 Da) and FGA (5904 Da); this indicates that ATP1B4 and FGA are associated with EM pathogenesis.

语种英语
WOS记录号WOS:000349523900018
项目编号30772319 ; 81170544 ; RDB2007-10
资助机构National Natural Science Foundation of China ; Peking University People&prime ; s Hospital Research and Development Funds
引用统计
被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/52956
专题北京大学第二临床医学院_妇科肿瘤中心
作者单位1.Peking Univ, Gynecol Oncol Ctr, Peoples Hosp, Beijing 100044, Peoples R China
2.Peking Univ, Ctr Reprod Med, Peoples Hosp, Beijing 100044, Peoples R China
推荐引用方式
GB/T 7714
Zhao, Yang,Liu, Ya-Nan,Li, Yi,et al. Identification of Biomarkers for Endometriosis Using Clinical Proteomics[J]. CHINESE MEDICAL JOURNAL,2015,128(4):520-527.
APA Zhao, Yang.,Liu, Ya-Nan.,Li, Yi.,Tian, Li.,Ye, Xue.,...&Chang, Xiao-Hong.(2015).Identification of Biomarkers for Endometriosis Using Clinical Proteomics.CHINESE MEDICAL JOURNAL,128(4),520-527.
MLA Zhao, Yang,et al."Identification of Biomarkers for Endometriosis Using Clinical Proteomics".CHINESE MEDICAL JOURNAL 128.4(2015):520-527.
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