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学科主题临床医学
Dextran-coated fluorapatite crystals doped with Yb3+/Ho3+ for labeling and tracking chondrogenic differentiation of bone marrow mesenchymal stem cells in vitro and in vivo
Hu, Xiaoqing1; Zhu, Jingxian1; Li, Xiyu2; Zhang, Xin1; Meng, Qingyang1; Yuan, Lan3; Zhang, Jiying1; Fu, Xin1; Duan, Xiaoning1; Chen, Haifeng2; Ao, Yingfang1
关键词Upconversion Fluorescence In Vivo imagIng Bmscs Chondrogenic Differentiation
刊名BIOMATERIALS
2015-06-01
DOI10.1016/j.biomaterials.2015.02.050
52页:441-451
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Engineering, Biomedical ; Materials Science, Biomaterials
资助者National Natural Science Foundation of China ; Specialized Research Fund for the Doctoral Program of Higher Education ; Leading Academic Discipline Project of Beijing Education Bureau ; National Basic Research Program of China ; National Natural Science Foundation of China ; Specialized Research Fund for the Doctoral Program of Higher Education ; Leading Academic Discipline Project of Beijing Education Bureau ; National Basic Research Program of China
研究领域[WOS]Engineering ; Materials Science
关键词[WOS]UP-CONVERSION NANOPARTICLES ; CLINICAL-TRIALS ; STROMAL CELLS ; FLUORESCENCE ; CARTILAGE ; DELIVERY ; THERAPY ; NANOPHOSPHORS ; NANOCRYSTALS ; LUMINESCENCE
英文摘要

Upconversion fluorescent nanoparticles are becoming more widely used as imaging contrast agents, owing to their high resolution and penetration depth, and avoidance of tissue auto-fluorescence and photodamage to cells. Here, we synthesized upconversion fluorescent crystals from rare-earth Yb3+ and Ho3+ co-doped fluorapatite (FA:Yb3+/Ho3+) suitable for long-term tracking and monitoring cartilage development (chondrogenesis) in bone marrow mesenchymal stem cells (BMSCs) in vitro and in vivo. We initially determined the structure, morphology and luminescence of the products using X-ray powder diffraction, transmission electron microscopy and two-photon confocal microscopy. When excited at 980 nm, FA:Yb3+/Ho3+ crystals exhibited distinct upconversion fluorescence peaks at 543 nm and 654 nm. We then conjugated FA:Yb3+/Ho3+ crystals with dextran to enhance hydrophilicity, biocompatibility and cell penetration. Next, we employed the dextran-coated FA:Yb3+/Ho3+ crystals in labeling and tracking chondrogenic differentiation processes in BMSCs stably expressing green fluorescent protein (BMSCsGFP) in vitro and in vivo. Labeled BMSCsGFP were shown to reproducibly exhibit chondrogenic differentiation potential in RT-PCR analysis, histological assessment and immunohistochemistry. We observed continuous luminescence from the FA:Yb3+/Ho3+ upconversion crystals at 4 weeks and 12 weeks post transplantation in BMSCsGFP, while GFP fluorescence in both control and crystal-treated groups significantly decreased at 12 weeks after BMSCsGFP transplantation. We therefore demonstrate the high biocompatibility and stability of FA:Yb3+/Ho3+ crystals in tracking and monitoring BMSCs chondrogenesis in vitro and in vivo, highlighting their excellent cell labeling potential in cartilage tissue engineering. (C) 2015 Elsevier Ltd. All rights reserved.

语种英语
所属项目编号90919022 ; 81101390 ; 81330040 ; 20110001130001 ; 2012CB933903
资助者National Natural Science Foundation of China ; Specialized Research Fund for the Doctoral Program of Higher Education ; Leading Academic Discipline Project of Beijing Education Bureau ; National Basic Research Program of China ; National Natural Science Foundation of China ; Specialized Research Fund for the Doctoral Program of Higher Education ; Leading Academic Discipline Project of Beijing Education Bureau ; National Basic Research Program of China
WOS记录号WOS:000353091000042
引用统计
被引频次:17[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/52957
专题北京大学第三临床医学院_运动医学研究所
作者单位1.Peking Univ, Beijing Key Lab Sports Injuries, Inst Sports Med, Hosp 3, Beijing 100191, Peoples R China
2.Peking Univ, Dept Biomed Engn, Coll Engn, Beijing 100871, Peoples R China
3.Peking Univ, Med & Hlth Anal Ctr, Beijing 100191, Peoples R China
推荐引用方式
GB/T 7714
Hu, Xiaoqing,Zhu, Jingxian,Li, Xiyu,et al. Dextran-coated fluorapatite crystals doped with Yb3+/Ho3+ for labeling and tracking chondrogenic differentiation of bone marrow mesenchymal stem cells in vitro and in vivo[J]. BIOMATERIALS,2015,52:441-451.
APA Hu, Xiaoqing.,Zhu, Jingxian.,Li, Xiyu.,Zhang, Xin.,Meng, Qingyang.,...&Ao, Yingfang.(2015).Dextran-coated fluorapatite crystals doped with Yb3+/Ho3+ for labeling and tracking chondrogenic differentiation of bone marrow mesenchymal stem cells in vitro and in vivo.BIOMATERIALS,52,441-451.
MLA Hu, Xiaoqing,et al."Dextran-coated fluorapatite crystals doped with Yb3+/Ho3+ for labeling and tracking chondrogenic differentiation of bone marrow mesenchymal stem cells in vitro and in vivo".BIOMATERIALS 52(2015):441-451.
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