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学科主题: 临床医学
题名:
Dextran-coated fluorapatite crystals doped with Yb3+/Ho3+ for labeling and tracking chondrogenic differentiation of bone marrow mesenchymal stem cells in vitro and in vivo
作者: Hu, Xiaoqing1; Zhu, Jingxian1; Li, Xiyu2; Zhang, Xin1; Meng, Qingyang1; Yuan, Lan3; Zhang, Jiying1; Fu, Xin1; Duan, Xiaoning1; Chen, Haifeng2; Ao, Yingfang1
关键词: Upconversion fluorescence ; In vivo imaging ; BMSCs ; Chondrogenic differentiation
刊名: BIOMATERIALS
发表日期: 2015-06-01
DOI: 10.1016/j.biomaterials.2015.02.050
卷: 52, 页:441-451
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Engineering, Biomedical ; Materials Science, Biomaterials
研究领域[WOS]: Engineering ; Materials Science
关键词[WOS]: UP-CONVERSION NANOPARTICLES ; CLINICAL-TRIALS ; STROMAL CELLS ; FLUORESCENCE ; CARTILAGE ; DELIVERY ; THERAPY ; NANOPHOSPHORS ; NANOCRYSTALS ; LUMINESCENCE
英文摘要:

Upconversion fluorescent nanoparticles are becoming more widely used as imaging contrast agents, owing to their high resolution and penetration depth, and avoidance of tissue auto-fluorescence and photodamage to cells. Here, we synthesized upconversion fluorescent crystals from rare-earth Yb3+ and Ho3+ co-doped fluorapatite (FA:Yb3+/Ho3+) suitable for long-term tracking and monitoring cartilage development (chondrogenesis) in bone marrow mesenchymal stem cells (BMSCs) in vitro and in vivo. We initially determined the structure, morphology and luminescence of the products using X-ray powder diffraction, transmission electron microscopy and two-photon confocal microscopy. When excited at 980 nm, FA:Yb3+/Ho3+ crystals exhibited distinct upconversion fluorescence peaks at 543 nm and 654 nm. We then conjugated FA:Yb3+/Ho3+ crystals with dextran to enhance hydrophilicity, biocompatibility and cell penetration. Next, we employed the dextran-coated FA:Yb3+/Ho3+ crystals in labeling and tracking chondrogenic differentiation processes in BMSCs stably expressing green fluorescent protein (BMSCsGFP) in vitro and in vivo. Labeled BMSCsGFP were shown to reproducibly exhibit chondrogenic differentiation potential in RT-PCR analysis, histological assessment and immunohistochemistry. We observed continuous luminescence from the FA:Yb3+/Ho3+ upconversion crystals at 4 weeks and 12 weeks post transplantation in BMSCsGFP, while GFP fluorescence in both control and crystal-treated groups significantly decreased at 12 weeks after BMSCsGFP transplantation. We therefore demonstrate the high biocompatibility and stability of FA:Yb3+/Ho3+ crystals in tracking and monitoring BMSCs chondrogenesis in vitro and in vivo, highlighting their excellent cell labeling potential in cartilage tissue engineering. (C) 2015 Elsevier Ltd. All rights reserved.

语种: 英语
所属项目编号: 90919022 ; 81101390 ; 81330040 ; 20110001130001 ; 2012CB933903
项目资助者: National Natural Science Foundation of China ; Specialized Research Fund for the Doctoral Program of Higher Education ; Leading Academic Discipline Project of Beijing Education Bureau ; National Basic Research Program of China
WOS记录号: WOS:000353091000042
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/52957
Appears in Collections:北京大学第三临床医学院_运动医学研究所_期刊论文

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作者单位: 1.Peking Univ, Beijing Key Lab Sports Injuries, Inst Sports Med, Hosp 3, Beijing 100191, Peoples R China
2.Peking Univ, Dept Biomed Engn, Coll Engn, Beijing 100871, Peoples R China
3.Peking Univ, Med & Hlth Anal Ctr, Beijing 100191, Peoples R China

Recommended Citation:
Hu, Xiaoqing,Zhu, Jingxian,Li, Xiyu,et al. Dextran-coated fluorapatite crystals doped with Yb3+/Ho3+ for labeling and tracking chondrogenic differentiation of bone marrow mesenchymal stem cells in vitro and in vivo[J]. BIOMATERIALS,2015,52:441-451.
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