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学科主题基础医学
PBX3 promotes migration and invasion of colorectal cancer cells via activation of MAPK/ERK signaling pathway
Han, Hai-Bo1; Gu, Jin2; Ji, Deng-Bo2; Li, Zhao-Wei2; Zhang, Yuan1; Zhao, Wei1; Wang, Li-Min1; Zhang, Zhi-Qian1
关键词Pre-b-cell Leukemia Homeobox 3 Colorectal Cancer Cell Migration Cell Invasion Mitogen-activated Protein Kinase Signaling Pathway
刊名WORLD JOURNAL OF GASTROENTEROLOGY
2014-12-28
DOI10.3748/wjg.v20.i48.18260
20期:48页:18260-18270
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Gastroenterology & Hepatology
研究领域[WOS]Gastroenterology & Hepatology
关键词[WOS]TRANSCRIPTION FACTORS ; PROSTATE-CANCER ; OVARIAN-CANCER ; TARGETING HOX ; PROTEINS ; PROLIFERATION ; EXPRESSION ; ANDROGEN ; GROWTH ; GENE
英文摘要

AIM: To investigate the role of pre-B-cell leukemia homeobox (PBX) 3 in migration and invasion of colorectal cancer (CRC) cells.

METHODS: We detected PBX3 expression in five cell lines and surgical specimens from 111 patients with CRC using real-time reverse transcription-polymerase chain reaction. We forced expression of PBX3 in low metastatic HT-29 and SW480 cells and knocked down expression of PBX3 in highly metastatic LOVO and HCT-8 cells. Wound healing and Boyden chamber assays were used to detect cell migration and invasion after altered expression of PBX3. Western blot was performed to detect the change of signaling molecule ERK1/2 following PBX3 overexpression.

RESULTS: High level of PBX3 expression was correlated with the invasive potential of CRC cells, and significantly associated with lymph node invasion (P = 0.02), distant metastasis (P = 0.04), advanced TNM stage (P = 0.03) and poor overall survival of patients (P < 0.05). Ectopic expression of PBX3 in low metastatic cells was shown to promote migration and invasion, while inhibited PBX3 expression in highly metastatic cells suppressed migration and invasion. Furthermore, upregulation of phosphorylated extracellular signal-regulated kinase (ERK) 1/2 was found to be one of the targeted molecules responsible for PBX3-induced CRC cell migration and invasion.

CONCLUSION: PBX3 induces invasion and metastasis of CRC cells partially through activation of the MAPK/ERK signaling pathway.

语种英语
WOS记录号WOS:000347460700017
项目编号5122012 ; 81201964 ; 81330051
资助机构Beijing Natural Science Foundation ; National Natural Science Foundation of China
引用统计
被引频次:20[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/52994
专题北京大学基础医学院_细胞生物学系
北京大学临床肿瘤学院_603课题组
北京大学临床肿瘤学院_结直肠肿瘤外科
作者单位1.Peking Univ Canc Hosp & Inst, Dept Cell Biol, Minist Educ, Key Lab Carcinogenesis & Translat Res, Beijing 100142, Peoples R China
2.Peking Univ Canc Hosp & Inst, Dept Colorectal Canc Surg, Beijing 100142, Peoples R China
推荐引用方式
GB/T 7714
Han, Hai-Bo,Gu, Jin,Ji, Deng-Bo,et al. PBX3 promotes migration and invasion of colorectal cancer cells via activation of MAPK/ERK signaling pathway[J]. WORLD JOURNAL OF GASTROENTEROLOGY,2014,20(48):18260-18270.
APA Han, Hai-Bo.,Gu, Jin.,Ji, Deng-Bo.,Li, Zhao-Wei.,Zhang, Yuan.,...&Zhang, Zhi-Qian.(2014).PBX3 promotes migration and invasion of colorectal cancer cells via activation of MAPK/ERK signaling pathway.WORLD JOURNAL OF GASTROENTEROLOGY,20(48),18260-18270.
MLA Han, Hai-Bo,et al."PBX3 promotes migration and invasion of colorectal cancer cells via activation of MAPK/ERK signaling pathway".WORLD JOURNAL OF GASTROENTEROLOGY 20.48(2014):18260-18270.
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