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学科主题: 基础医学
题名:
PBX3 promotes migration and invasion of colorectal cancer cells via activation of MAPK/ERK signaling pathway
作者: Han, Hai-Bo1; Gu, Jin2; Ji, Deng-Bo2; Li, Zhao-Wei2; Zhang, Yuan1; Zhao, Wei1; Wang, Li-Min1; Zhang, Zhi-Qian1
关键词: Pre-B-cell leukemia homeobox 3 ; Colorectal cancer ; Cell migration ; Cell invasion ; Mitogen-activated protein kinase signaling pathway
刊名: WORLD JOURNAL OF GASTROENTEROLOGY
发表日期: 2014-12-28
DOI: 10.3748/wjg.v20.i48.18260
卷: 20, 期:48, 页:18260-18270
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Gastroenterology & Hepatology
研究领域[WOS]: Gastroenterology & Hepatology
关键词[WOS]: TRANSCRIPTION FACTORS ; PROSTATE-CANCER ; OVARIAN-CANCER ; TARGETING HOX ; PROTEINS ; PROLIFERATION ; EXPRESSION ; ANDROGEN ; GROWTH ; GENE
英文摘要:

AIM: To investigate the role of pre-B-cell leukemia homeobox (PBX) 3 in migration and invasion of colorectal cancer (CRC) cells.

METHODS: We detected PBX3 expression in five cell lines and surgical specimens from 111 patients with CRC using real-time reverse transcription-polymerase chain reaction. We forced expression of PBX3 in low metastatic HT-29 and SW480 cells and knocked down expression of PBX3 in highly metastatic LOVO and HCT-8 cells. Wound healing and Boyden chamber assays were used to detect cell migration and invasion after altered expression of PBX3. Western blot was performed to detect the change of signaling molecule ERK1/2 following PBX3 overexpression.

RESULTS: High level of PBX3 expression was correlated with the invasive potential of CRC cells, and significantly associated with lymph node invasion (P = 0.02), distant metastasis (P = 0.04), advanced TNM stage (P = 0.03) and poor overall survival of patients (P < 0.05). Ectopic expression of PBX3 in low metastatic cells was shown to promote migration and invasion, while inhibited PBX3 expression in highly metastatic cells suppressed migration and invasion. Furthermore, upregulation of phosphorylated extracellular signal-regulated kinase (ERK) 1/2 was found to be one of the targeted molecules responsible for PBX3-induced CRC cell migration and invasion.

CONCLUSION: PBX3 induces invasion and metastasis of CRC cells partially through activation of the MAPK/ERK signaling pathway.

语种: 英语
所属项目编号: 5122012 ; 81201964 ; 81330051
项目资助者: Beijing Natural Science Foundation ; National Natural Science Foundation of China
WOS记录号: WOS:000347460700017
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/52994
Appears in Collections:基础医学院_细胞生物学系_期刊论文

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作者单位: 1.Peking Univ Canc Hosp & Inst, Dept Cell Biol, Minist Educ, Key Lab Carcinogenesis & Translat Res, Beijing 100142, Peoples R China
2.Peking Univ Canc Hosp & Inst, Dept Colorectal Canc Surg, Beijing 100142, Peoples R China

Recommended Citation:
Han, Hai-Bo,Gu, Jin,Ji, Deng-Bo,et al. PBX3 promotes migration and invasion of colorectal cancer cells via activation of MAPK/ERK signaling pathway[J]. WORLD JOURNAL OF GASTROENTEROLOGY,2014,20(48):18260-18270.
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