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Activation of the cAMP-PKA signaling pathway in rat dorsal root ganglion and spinal cord contributes toward induction and maintenance of bone cancer pain
Zhu, Gui-Qin1; Liu, Su1,2; He, Duan-Duan3; Liu, Yue-Peng1,2; Song, Xue-Jun1,4,5
关键词Bone Cancer Pain Cyclic Adenosine Monophosphate Dorsal Root Ganglia Protein Kinase a Rat Spinal Cord
刊名BEHAVIOURAL PHARMACOLOGY
2014-08-01
DOI10.1097/FBP.0000000000000048
25期:4页:267-276
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Behavioral Sciences ; Neurosciences ; Pharmacology & Pharmacy
研究领域[WOS]Behavioral Sciences ; Neurosciences & Neurology ; Pharmacology & Pharmacy
关键词[WOS]PROTEIN-KINASE-A ; SENSORY NEURONS ; CYCLIC-AMP ; ANTINOCICEPTIVE TOLERANCE ; THERMAL HYPERALGESIA ; TRANSDUCTION CASCADE ; SYNAPTIC PLASTICITY ; CHRONIC COMPRESSION ; INFLAMMATORY PAIN ; NEUROPATHIC PAIN
英文摘要

The objective of this study was to explore the role of cyclic adenosine monophosphate-protein kinase A (cAMP-PKA) signaling in the development of bone cancer pain in rats. Female Sprague-Dawley rats (N = 48) were divided randomly into four groups: sham (n = 8), tumor cell implantation (TCI) (n = 16), TCI+ saline (n = 8), and TCI + PKA inhibitor (n = 16). Bone cancer-induced pain behaviors - thermal hyperalgesia and mechanical allodynia - were tested at postoperative days -3, -1, 1, 3, 5, 7, 10, and 14. A PKA inhibitor, Rp-cAMPS (1 mmol/l/20 mu l), was injected intrathecally on postoperative days 3, 4, and 5 (early phase) or 7, 8, and 9 postoperative days (late phase). The expression of PKA mRNA in dorsal root ganglia (DRG) was detected by reverse transcription-PCR. The concentration of cAMP and activity of PKA in DRG and spinal cord were measured by enzyme-linked immunosorbent assay. TCI treatment induced significant pain behaviors, manifested as thermal hyperalgesia and mechanical allodynia. Spinal administration of the PKA inhibitor Rp-cAMPS during the early phase and late phase significantly delayed or reversed, respectively, TCI-induced thermal hyperalgesia and mechanical allodynia. TCI treatment also led to obvious tumor growth and bone destruction. The level of PKA mRNA in the DRG, as well as the concentration of cAMP and the activity of PKA, in both the DRG and spinal cord were significantly increased after TCI treatment (P < 0.01). We conclude that the inhibition of the cAMP-PKA signaling pathway may reduce bone cancer pain. (C) 2014 Wolters Kluwer Health vertical bar Lippincott Williams & Wilkins.

语种英语
WOS记录号WOS:000339258500002
引用统计
被引频次:8[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/53002
专题北京大学第三临床医学院_麻醉科
作者单位1.Liangyungang City Dongfang Hosp, Ctr Clin Res & Translat Med, Dept Anesthesiol, Liangyungang, Jiangsu, Peoples R China
2.Xuzhou Med Coll, Dept Anesthesiol, Affiliated Hosp, Xuzhou 221002, Jiangsu, Peoples R China
3.Peking Univ, Affiliated Hosp 3, Dept Anesthesiol, Beijing 100871, Peoples R China
4.Peking Univ, Neurosci Res Inst, Beijing 100871, Peoples R China
5.Peking Univ, Ctr Pain Med, Beijing 100871, Peoples R China
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Zhu, Gui-Qin,Liu, Su,He, Duan-Duan,et al. Activation of the cAMP-PKA signaling pathway in rat dorsal root ganglion and spinal cord contributes toward induction and maintenance of bone cancer pain[J]. BEHAVIOURAL PHARMACOLOGY,2014,25(4):267-276.
APA Zhu, Gui-Qin,Liu, Su,He, Duan-Duan,Liu, Yue-Peng,&Song, Xue-Jun.(2014).Activation of the cAMP-PKA signaling pathway in rat dorsal root ganglion and spinal cord contributes toward induction and maintenance of bone cancer pain.BEHAVIOURAL PHARMACOLOGY,25(4),267-276.
MLA Zhu, Gui-Qin,et al."Activation of the cAMP-PKA signaling pathway in rat dorsal root ganglion and spinal cord contributes toward induction and maintenance of bone cancer pain".BEHAVIOURAL PHARMACOLOGY 25.4(2014):267-276.
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