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学科主题: 临床医学
题名:
Serum-free media for articular chondrocytes in vitro expansion
作者: Shao Xin-xin1,2,3; Duncan, Neil A.1,2; Lin Lin3; Fu Xin3; Zhang Ji-ying3; Yu Chang-long3
关键词: articular chondrocyte ; serum-free media ; in vitro
刊名: CHINESE MEDICAL JOURNAL
发表日期: 2013-07-05
DOI: 10.3760/cma.j.issn.0366-6999.20112153
卷: 126, 期:13, 页:2523-2529
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medicine, General & Internal
研究领域[WOS]: General & Internal Medicine
关键词[WOS]: GROWTH-FACTORS ; CARTILAGE REPAIR ; CELL-ADHESION ; FIBRONECTIN ; CULTURE ; TRANSPLANTATION ; MATRIX ; REDIFFERENTIATION ; DEDIFFERENTIATION ; DIFFERENTIATION
英文摘要:

Background In vitro chondrocyte expansion is a major challenge in cell-based therapy for human articular cartilage repair. Classical culture conditions usually use animal serum as a medium supplement, which raises a number of undesirable questions. In the present study, two kinds of defined, serum-free media were developed to expand chondrocytes in monolayer culture for the purpose of cartilage tissue engineering.

Methods Bovine chondrocytes were expanded in serum-free media supplemented with fibroblast growth factor-2 and platelet-derived growth factor or fibroblast growth factor-2 and insulin-like growth factor. Expansion culture in a conventional 10% fetal bovine serum (FBS) medium served as control. Fibronectin coating was used to help cell adhesion in serum-free medium. Next, in vitro three-dimensional pellet culture was used to evaluate the chondrocyte capacity. Cell pellets were expanded in different media to re-express the differentiated phenotype (re-differentiation) and to form cartilaginous tissue. The pellets were assessed by glycosaminoglycans contents, collagen II, collagen I and collagen X immunohistological staining.

Results Chondrocytes cultured in serum-free media showed no proliferation difference than cells grown with 10% FBS medium. In addition, chondrocytes expanded in both serum-free media expressed more differentiated phenotypes at the end of monolayer culture, as indicated by higher gene expression ratios of collagen type II to collagen type I. Pellets derived from chondrocytes cultured in both serum-free media displayed comparable chondrogenic capacities to pellets from cells expanded in 10% FBS medium.

Conclusion These findings provide alternative culture approaches for chondrocytes in vitro expansion, which may benefit the clinical use of autologous chondrocytes implantation.

语种: 英语
WOS记录号: WOS:000329015200022
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/53003
Appears in Collections:北京大学第三临床医学院_运动医学研究所_期刊论文

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作者单位: 1.Univ Calgary, McCaig Inst Bone & Joint Hlth, Calgary, AB, Canada
2.Univ Calgary, Schulich Sch Engn, Calgary, AB, Canada
3.Peking Univ, Hosp 3, Inst Sports Med, Beijing 100083, Peoples R China

Recommended Citation:
Shao Xin-xin,Duncan, Neil A.,Lin Lin,et al. Serum-free media for articular chondrocytes in vitro expansion[J]. CHINESE MEDICAL JOURNAL,2013,126(13):2523-2529.
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