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Heterogeneous populations of microglia/macrophages in the retina and their activation after retinal ischemia and reperfusion injury
Zhang, C1; Lam, TT1; Tso, MOM1
关键词Retinal Ischemia Microglia/macrophage Immunohistochemistry
刊名EXPERIMENTAL EYE RESEARCH
2005-12-01
DOI10.1016/j.exer.2005.04.008
81期:6页:700-709
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Ophthalmology
研究领域[WOS]Ophthalmology
关键词[WOS]FOCAL CEREBRAL-ISCHEMIA ; MICROGLIAL RESPONSE ; RAT-BRAIN ; RECEPTOR ANTAGONIST ; HEMATOPOIETIC-CELLS ; TEMPORAL PROFILE ; MIGRATION ; PROTEIN ; CHEMOATTRACTANT ; LOCALIZATION
英文摘要

Activation of Microglia/macrophages has been observed in ischemia-reperfusion injury of the brain. This study was undertaken to investigate the different subpopulations of microglia/macrophages in the normal rat retina and their activation after retinal ischemia. Retinal ischemia was induced by elevation of intraocular pressure to 120 mmHg for 60 min. Microglia/macrophages were identified on frozen retinal sections by four antibodies, namely OX42, 5D4, OX6 and ED1. In the normal retina, there were heterogeneous populations of resident microglia/macrophages as characterized by their differences in morphology, antigen expression and distribution. OX42+ cells had delicate processes and were located in the inner layers of the retina, while 5D4+ cells were highly ramified and mostly scattered in the inner plexiform layer (IPL) and the outer plexiform layer. Few amoeboid ED1+ cells were also seen in the ganglion cell layer and IPL. OX6+ (MHC-II antigen presenting) cells were not detected in the normal retinas. Double labeling with OX42 and 5D4 antibodies on normal retinal sections showed few microglia exhibited positive labeling with both OX42 and 5134, while the majority of the microglia were labeled with either OX42 or 5D4 antibodies. After retinal ischemia single labeling with these antibodies showed increased number of these antigen-expressing cells, disappearance of normal cellular processes, and rounding or amoeboid like appearance of the cell bodies. At I day after ischemia, there was a significant infiltration of round OX42+, ED1+ and OX6+ cells with loss of the cellular processes in the inner retina. From 3 to 14 days, all subpopulations of microglia/macrophages differentiated cellular processes and became dendritic again. Double labeling on retinas after I day of recovery showed OX42+ cells were co-labeled with ED1+ or OX6+ cells, but not with 5D4+ cells. Scattered amoeboid OX42+, 5D4+, and ED1+ cells were noted in the subretinal space 3-14 days after ischemia. In summary, there were heterogeneous populations of resident microglia/macrophages in the normal inner retina and they were activated early after ischemia-reperfusion injury and exhibited different antigenic expression which were further altered in the recovery phase. (c) 2005 Elsevier Ltd. All rights reserved.

语种英语
WOS记录号WOS:000234332700010
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被引频次:69[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/53018
专题北京大学第三临床医学院_眼科
作者单位1.Johns Hopkins Univ, Sch Med, Wilmer Eye Inst, Baltimore, MD 21287 USA
2.Univ So Calif, Keck Sch Med, Doheny Eye Inst, Dept Ophthalmol, Los Angeles, CA 90033 USA
3.Peking Univ, Hosp 3, Ctr Eye, Beijing 100871, Peoples R China
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Zhang, C,Lam, TT,Tso, MOM. Heterogeneous populations of microglia/macrophages in the retina and their activation after retinal ischemia and reperfusion injury[J]. EXPERIMENTAL EYE RESEARCH,2005,81(6):700-709.
APA Zhang, C,Lam, TT,&Tso, MOM.(2005).Heterogeneous populations of microglia/macrophages in the retina and their activation after retinal ischemia and reperfusion injury.EXPERIMENTAL EYE RESEARCH,81(6),700-709.
MLA Zhang, C,et al."Heterogeneous populations of microglia/macrophages in the retina and their activation after retinal ischemia and reperfusion injury".EXPERIMENTAL EYE RESEARCH 81.6(2005):700-709.
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