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学科主题: 临床医学
题名:
Endothelial cell chimerism by fluorescence in situ hybridization in gender mismatched renal allograft biopsies
作者: Bai Hong-wei1; Shi Bing-yi1; Qian Ye-yong1; Na Yan-Qun1; Zeng Xuan1; Zhong Ding-rong1; Lu Min1; Zou Wan-zhong1; Wu Sha-fei1
关键词: kidney transplantation ; in situ hybridization ; fluorescence ; endothelium ; vascular ; chimerism ; cold ischemia
刊名: CHINESE MEDICAL JOURNAL
发表日期: 2007-05-20
卷: 120, 期:10, 页:859-862
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medicine, General & Internal
研究领域[WOS]: General & Internal Medicine
关键词[WOS]: Y-CHROMOSOME ; INSITU HYBRIDIZATION ; TRANSPLANTATION ; ANTIBODIES ; REJECTION ; XENOGRAFTS ; RECIPIENTS ; TURNOVER ; HEARTS
英文摘要:

Background The blood vessels of a transplanted organ are the interface between donor and recipient. The endothelium in the blood vessels is thought to be the major target for graft rejection. Endothelial cells of a transplanted organ can be of recipient origin after transplantation. In this study, we tested whether endothelial chimerism correlated with the graft rejection and cold ischemia.

Methods We studied the biopsy samples from 34 renal transplants of female recipients who received the kidney from a male donor for the presence of endothelial cells of recipient origin. We examined the tissue sections of renal biopsy samples by fluorescence in situ hybridization (FISH) for the presence of endothelial cells containing two X chromosomes using a biotinylated Y chromosome probe and digoxigenin labelled X chromosome probe, and then analyzed the relationship between the endothelial cell chimerism and the rejection and cold ischemia.

Results Endothelial chimerism was common and irrespective of rejections (P > 0.05). The cold ischemic time of chimerism group was longer than no chimerism group ((14.83 +/- 4.03) hours vs (11.27 +/- 3.87) hours, P < 0.05).

Conclusions There is no correlation between the percentage of recipient endothelial cells in vascular endothelial cells and the type of graft rejection. The endothelium damaged by ischemic injury might be repaired by the endothelial cells from the recipient.

语种: 英语
WOS记录号: WOS:000246829400001
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/53019
Appears in Collections:北京大学第一临床医学院_泌尿外科_期刊论文

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作者单位: 1.Peoples Liberat Army Gen Hosp, Transplantat Ctr PLA, Affiliated Hosp 2, Beijing 100091, Peoples R China
2.Peking Univ, Dept Urol, Inst Urol, Beijing 100034, Peoples R China
3.Beijing Union Med Coll Hosp, Dept Pathol, Beijing 100730, Peoples R China
4.Chinese Acad Med Sci, Dept Pathol, Beijing 100730, Peoples R China
5.Peking Univ, Ctr Hlth Sci, Dept Pathol, Beijing 100083, Peoples R China

Recommended Citation:
Bai Hong-wei,Shi Bing-yi,Qian Ye-yong,et al. Endothelial cell chimerism by fluorescence in situ hybridization in gender mismatched renal allograft biopsies[J]. CHINESE MEDICAL JOURNAL,2007,120(10):859-862.
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