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学科主题: 药学
题名:
Metal ions induced heat shock protein response by elevating superoxide anion level in HeLa cells transformed by HSE-SEAP reporter gene
作者: Yu, ZJ1; Yang, XD1; Wang, K1
关键词: heat shock protein (HSP) ; reporter gene ; secreted alkaline phosphatase (SEAP) ; reactive oxygen species (ROS) ; metal ions
刊名: TOXICOLOGY
发表日期: 2006-06-01
DOI: 10.1016/j.tox.2006.02.021
卷: 223, 期:1-2, 页:1-8
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Pharmacology & Pharmacy ; Toxicology
研究领域[WOS]: Pharmacology & Pharmacy ; Toxicology
关键词[WOS]: OXIDATIVE STRESS ; SODIUM ARSENITE ; FREE-RADICALS ; EXPRESSION ; INDUCTION ; ACTIVATION ; APOPTOSIS ; CADMIUM ; HEAT-SHOCK-PROTEIN-70 ; DROSOPHILA
英文摘要:

The aim of this work is to define the relationship between heat shock protein (HSP) and reactive oxygen species (ROS) in the cells exposed to different concentrations of metal ions, and to evaluate a new method for tracing the dynamic levels of cellular reactive oxygen species using a HSE-SEAP reporter gene. The expression of heat shock protein was measured using a secreted alkaline phosphatase (SEAP) reporter gene transformed into HeLa cell strain, the levels of superoxide anion (O-center dot(2)-) and hydrogen peroxide (H2O2) were determined by NBT reduction assay and DCFH staining flow cytometry (FCM), respectively. The experimental results demonstrated that the expression of heat shock protein induced by metal ions was linearly related to the cellular superoxide anion level before cytotoxic effects were observed, but not related to the cellular hydrogen peroxide level. The experimental results suggested that metal ions might induce heat shock protein by elevating cellular superoxide anion level, and thus the expression of heat shock protein indicated by the HSE-SEAP reporter gene can be an effective model for monitoring the dynamic level of superoxide anion and early metal-induced oxidative stress/cytotoxicity. (c) 2006 Elsevier Ireland Ltd. All rights reserved.

语种: 英语
WOS记录号: WOS:000238346300001
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/53031
Appears in Collections:北京大学药学院_化学生物学系_期刊论文

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作者单位: 1.Peking Univ, Hlth Sci Ctr, Dept Biol Chem, Sch Pharmaceut Sci, Beijing 100083, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Natl Lab Nat & Biomimetr Drugs, Sch Pharmaceut Sci, Beijing 100083, Peoples R China

Recommended Citation:
Yu, ZJ,Yang, XD,Wang, K. Metal ions induced heat shock protein response by elevating superoxide anion level in HeLa cells transformed by HSE-SEAP reporter gene[J]. TOXICOLOGY,2006,223(1-2):1-8.
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