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Metal ions induced heat shock protein response by elevating superoxide anion level in HeLa cells transformed by HSE-SEAP reporter gene
Yu, ZJ1; Yang, XD1; Wang, K1
关键词Heat Shock Protein (Hsp) Reporter Gene Secreted Alkaline Phosphatase (Seap) Reactive Oxygen Species (Ros) Metal Ions
刊名TOXICOLOGY
2006-06-01
DOI10.1016/j.tox.2006.02.021
223期:1-2页:1-8
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Pharmacology & Pharmacy ; Toxicology
研究领域[WOS]Pharmacology & Pharmacy ; Toxicology
关键词[WOS]OXIDATIVE STRESS ; SODIUM ARSENITE ; FREE-RADICALS ; EXPRESSION ; INDUCTION ; ACTIVATION ; APOPTOSIS ; CADMIUM ; HEAT-SHOCK-PROTEIN-70 ; DROSOPHILA
英文摘要

The aim of this work is to define the relationship between heat shock protein (HSP) and reactive oxygen species (ROS) in the cells exposed to different concentrations of metal ions, and to evaluate a new method for tracing the dynamic levels of cellular reactive oxygen species using a HSE-SEAP reporter gene. The expression of heat shock protein was measured using a secreted alkaline phosphatase (SEAP) reporter gene transformed into HeLa cell strain, the levels of superoxide anion (O-center dot(2)-) and hydrogen peroxide (H2O2) were determined by NBT reduction assay and DCFH staining flow cytometry (FCM), respectively. The experimental results demonstrated that the expression of heat shock protein induced by metal ions was linearly related to the cellular superoxide anion level before cytotoxic effects were observed, but not related to the cellular hydrogen peroxide level. The experimental results suggested that metal ions might induce heat shock protein by elevating cellular superoxide anion level, and thus the expression of heat shock protein indicated by the HSE-SEAP reporter gene can be an effective model for monitoring the dynamic level of superoxide anion and early metal-induced oxidative stress/cytotoxicity. (c) 2006 Elsevier Ireland Ltd. All rights reserved.

语种英语
WOS记录号WOS:000238346300001
引用统计
被引频次:11[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/53031
专题北京大学药学院_化学生物学系
作者单位1.Peking Univ, Hlth Sci Ctr, Dept Biol Chem, Sch Pharmaceut Sci, Beijing 100083, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Natl Lab Nat & Biomimetr Drugs, Sch Pharmaceut Sci, Beijing 100083, Peoples R China
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Yu, ZJ,Yang, XD,Wang, K. Metal ions induced heat shock protein response by elevating superoxide anion level in HeLa cells transformed by HSE-SEAP reporter gene[J]. TOXICOLOGY,2006,223(1-2):1-8.
APA Yu, ZJ,Yang, XD,&Wang, K.(2006).Metal ions induced heat shock protein response by elevating superoxide anion level in HeLa cells transformed by HSE-SEAP reporter gene.TOXICOLOGY,223(1-2),1-8.
MLA Yu, ZJ,et al."Metal ions induced heat shock protein response by elevating superoxide anion level in HeLa cells transformed by HSE-SEAP reporter gene".TOXICOLOGY 223.1-2(2006):1-8.
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