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学科主题临床医学
The vasorelaxing effect of hydrogen sulfide on isolated rat aortic rings versus pulmonary artery rings
Sun, Yan1; Tang, Chao-shu2,3; Jin, Hong-fang1; Du, Jun-bao1,2
关键词Hydrogen Sulfide Aortic Rings Pulmonary Rings Vasorelaxation Cystathinonine Gamma-lyase Cystathionine Beta Synthase 3-mercapto Pyruvate Sulfurtransferase Glibenclamide 5-hydroxydecanoate
刊名ACTA PHARMACOLOGICA SINICA
2011-04-01
DOI10.1038/aps.2011.9
32期:4页:456-464
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Chemistry, Multidisciplinary ; Pharmacology & Pharmacy
资助者Foundation of the Ministry of Education, People&prime ; s Republic of China ; National Natural Science Foundation of China ; Major Basic Research Development Program of the People&prime ; s Republic of China ; Beijing Natural Science Foundation ; Foundation of the Ministry of Education, People&prime ; s Republic of China ; National Natural Science Foundation of China ; Major Basic Research Development Program of the People&prime ; s Republic of China ; Beijing Natural Science Foundation
研究领域[WOS]Chemistry ; Pharmacology & Pharmacy
关键词[WOS]K-ATP CHANNELS ; SMOOTH-MUSCLE ; CARDIOVASCULAR-SYSTEM ; CARDIAC-FUNCTION ; NITRIC-OXIDE ; GAMMA-LYASE ; H2S ; HYPERTENSION ; CELLS ; VASODILATION
英文摘要

Aim: To compare the vasorelaxing effects of hydrogen sulfide (H(2)S) on isolated aortic and pulmonary artery rings and to determine their action mechanisms.

Methods: H(2)S-induced vasorelaxation of isolated rat aortic versus pulmonary artery rings under 95% O(2) and 5% CO(2) was analyzed. The expression of cystathinonine gamma-lyase (CSE), cystathionine beta synthase (CBS), 3-mercaptopyruvate sulfurtransferase (3MST), SUR2B and Kir6.1 was examined.

Results: NaHS caused vasorelaxation of rat aortic and pulmonary artery rings in a dose-dependent manner. NaHS dilated aortic rings to a greater extent (16.4%, 38.4%, 64.1%, 84.3%, and 95.9% at concentrations of 50, 100, 200, 500, and 1000 mu mol/L, respectively) than pulmonary artery rings (10.1%, 22.2%, 50.6%, 73.6%, and 84.6% at concentrations of 50, 100, 200, 500 and 1000 mu mol/L, respectively). The EC(50) of the vasorelaxant effect for aortic rings was 152.17 mu mol/L, whereas the EC50 for pulmonary artery rings was 233.65 mu mol/L. The vasorelaxing effect of H(2)S was markedly blocked by cellular and mitochondrial membrane K(ATP) channel blockers in aortic rings (P < 0.01). In contrast, only the cellular membrane K(ATP) channel blocker inhibited H(2)S-induced vasorelaxation in pulmonary artery rings. SUR2B mRNA and protein expression was higher in aortic rings than in pulmonary artery rings. Cystathinonine gamma-lyase (CSE) but not cystathionine beta synthase (CBS) expression in aortic rings was higher than in pulmonary artery rings. 3-Mercapto pyruvate sulfurtransferase (3MST) mRNA was lower in aortic rings than in pulmonary artery rings.

Conclusion: The vasorelaxing effect of H(2)S on isolated aortic rings was more pronounced than the effect on pulmonary artery rings at specific concentrations, which might be associated with increased expression of the K(ATP) channel subunit SUR2B.

语种英语
所属项目编号20070001702 ; 20070001770 ; 81070212 ; 30821001 ; 30801251 ; 2011CB503904 ; 7082095
资助者Foundation of the Ministry of Education, People&prime ; s Republic of China ; National Natural Science Foundation of China ; Major Basic Research Development Program of the People&prime ; s Republic of China ; Beijing Natural Science Foundation ; Foundation of the Ministry of Education, People&prime ; s Republic of China ; National Natural Science Foundation of China ; Major Basic Research Development Program of the People&prime ; s Republic of China ; Beijing Natural Science Foundation
WOS记录号WOS:000289194200007
引用统计
被引频次:15[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/53038
专题北京大学第一临床医学院_儿科
作者单位1.Minist Educ, Key Lab Mol Cardiol, Beijing 100083, Peoples R China
2.Peking Univ, Hosp 1, Dept Pediat, Beijing 100034, Peoples R China
3.Peking Univ, Hosp 1, Inst Cardiovasc Res, Beijing 100034, Peoples R China
推荐引用方式
GB/T 7714
Sun, Yan,Tang, Chao-shu,Jin, Hong-fang,et al. The vasorelaxing effect of hydrogen sulfide on isolated rat aortic rings versus pulmonary artery rings[J]. ACTA PHARMACOLOGICA SINICA,2011,32(4):456-464.
APA Sun, Yan,Tang, Chao-shu,Jin, Hong-fang,&Du, Jun-bao.(2011).The vasorelaxing effect of hydrogen sulfide on isolated rat aortic rings versus pulmonary artery rings.ACTA PHARMACOLOGICA SINICA,32(4),456-464.
MLA Sun, Yan,et al."The vasorelaxing effect of hydrogen sulfide on isolated rat aortic rings versus pulmonary artery rings".ACTA PHARMACOLOGICA SINICA 32.4(2011):456-464.
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