|The vasorelaxing effect of hydrogen sulfide on isolated rat aortic rings versus pulmonary artery rings|
|Sun, Yan1; Tang, Chao-shu2,3; Jin, Hong-fang1; Du, Jun-bao1,2|
|关键词||Hydrogen Sulfide Aortic Rings Pulmonary Rings Vasorelaxation Cystathinonine Gamma-lyase Cystathionine Beta Synthase 3-mercapto Pyruvate Sulfurtransferase Glibenclamide 5-hydroxydecanoate|
|刊名||ACTA PHARMACOLOGICA SINICA|
|WOS标题词||Science & Technology|
|类目[WOS]||Chemistry, Multidisciplinary ; Pharmacology & Pharmacy|
|研究领域[WOS]||Chemistry ; Pharmacology & Pharmacy|
|关键词[WOS]||K-ATP CHANNELS ; SMOOTH-MUSCLE ; CARDIOVASCULAR-SYSTEM ; CARDIAC-FUNCTION ; NITRIC-OXIDE ; GAMMA-LYASE ; H2S ; HYPERTENSION ; CELLS ; VASODILATION|
Aim: To compare the vasorelaxing effects of hydrogen sulfide (H(2)S) on isolated aortic and pulmonary artery rings and to determine their action mechanisms.
Methods: H(2)S-induced vasorelaxation of isolated rat aortic versus pulmonary artery rings under 95% O(2) and 5% CO(2) was analyzed. The expression of cystathinonine gamma-lyase (CSE), cystathionine beta synthase (CBS), 3-mercaptopyruvate sulfurtransferase (3MST), SUR2B and Kir6.1 was examined.
Results: NaHS caused vasorelaxation of rat aortic and pulmonary artery rings in a dose-dependent manner. NaHS dilated aortic rings to a greater extent (16.4%, 38.4%, 64.1%, 84.3%, and 95.9% at concentrations of 50, 100, 200, 500, and 1000 mu mol/L, respectively) than pulmonary artery rings (10.1%, 22.2%, 50.6%, 73.6%, and 84.6% at concentrations of 50, 100, 200, 500 and 1000 mu mol/L, respectively). The EC(50) of the vasorelaxant effect for aortic rings was 152.17 mu mol/L, whereas the EC50 for pulmonary artery rings was 233.65 mu mol/L. The vasorelaxing effect of H(2)S was markedly blocked by cellular and mitochondrial membrane K(ATP) channel blockers in aortic rings (P < 0.01). In contrast, only the cellular membrane K(ATP) channel blocker inhibited H(2)S-induced vasorelaxation in pulmonary artery rings. SUR2B mRNA and protein expression was higher in aortic rings than in pulmonary artery rings. Cystathinonine gamma-lyase (CSE) but not cystathionine beta synthase (CBS) expression in aortic rings was higher than in pulmonary artery rings. 3-Mercapto pyruvate sulfurtransferase (3MST) mRNA was lower in aortic rings than in pulmonary artery rings.
Conclusion: The vasorelaxing effect of H(2)S on isolated aortic rings was more pronounced than the effect on pulmonary artery rings at specific concentrations, which might be associated with increased expression of the K(ATP) channel subunit SUR2B.
|项目编号||20070001702 ; 20070001770 ; 81070212 ; 30821001 ; 30801251 ; 2011CB503904 ; 7082095|
|资助机构||Foundation of the Ministry of Education, People&prime ; s Republic of China ; National Natural Science Foundation of China ; Major Basic Research Development Program of the People&prime ; s Republic of China ; Beijing Natural Science Foundation|
|作者单位||1.Minist Educ, Key Lab Mol Cardiol, Beijing 100083, Peoples R China|
2.Peking Univ, Hosp 1, Dept Pediat, Beijing 100034, Peoples R China
3.Peking Univ, Hosp 1, Inst Cardiovasc Res, Beijing 100034, Peoples R China
|Sun, Yan,Tang, Chao-shu,Jin, Hong-fang,et al. The vasorelaxing effect of hydrogen sulfide on isolated rat aortic rings versus pulmonary artery rings[J]. ACTA PHARMACOLOGICA SINICA,2011,32(4):456-464.|
|APA||Sun, Yan,Tang, Chao-shu,Jin, Hong-fang,&Du, Jun-bao.(2011).The vasorelaxing effect of hydrogen sulfide on isolated rat aortic rings versus pulmonary artery rings.ACTA PHARMACOLOGICA SINICA,32(4),456-464.|
|MLA||Sun, Yan,et al."The vasorelaxing effect of hydrogen sulfide on isolated rat aortic rings versus pulmonary artery rings".ACTA PHARMACOLOGICA SINICA 32.4(2011):456-464.|