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学科主题: 临床医学
题名:
Simultaneous quantitation of dexamethasone palmitate and dexamethasone in human plasma by liquid chromatography/tandem mass spectrometry
作者: Yang, Yan1; Li, Hao1; Gao, Kan1; Liu, Mingyuan1,2; Sun, Yantong1; Yan, Tingting1; Fawcett, J. Paul3; Cui, Yimin4; Gu, Jingkai1
关键词: dexamethasone ; dexamethasone palmitate ; LC/MS/MS ; pharmacokinetics ; human
刊名: JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
发表日期: 2008-02-01
DOI: 10.1016/j.jchromb.2007.11.033
卷: 862, 期:1-2, 页:119-124
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]: GAS-CHROMATOGRAPHY ; QUANTIFICATION ; BETAMETHASONE ; VALIDATION ; PHOSPHATE ; URINE
英文摘要:

A rapid and sensitive liquid chromatography/tandem mass spectrometry (LC/MS/MS) method for simultaneous quantitation of dexamethasone palmitate and dexamethasone in human plasma was developed. After sample preparation by protein precipitation and liquid-liquid extraction, the analytes and internal standard (IS) were separated on a Venusil XBP-C-8 column using gradient elution. Multiple reaction monitoring of dexamethasone palmitate, dexamethasone and IS used the precursor to product ion transitions at m/z 631.8 -> 373.1, m/z 393.2 -> 147.1 and m/z 264.2 -> 58.1, respectively. The method was linear over the ranges 1.5-1000 ng/mL for dexamethasone palmitate and 2.5-250 ng/mL for dexamethasone with intra- and inter-day precisions of <10% and accuracies of 100 +/- 7%. The assay was applied to a clinical pharmacokinetic study involving the injection of dexamethasone palmitate to healthy volunteers. (c) 2007 Elsevier B.V. All rights reserved.

语种: 英语
WOS记录号: WOS:000253354200013
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/53055
Appears in Collections:北京大学第一临床医学院_药剂科_期刊论文

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作者单位: 1.Jilin Univ, Coll Life Sci, Res Ctr Drug Metab, Changchun 130021, Peoples R China
2.Jiamusi Univ, Dept Pharmacol, Sch Basic Med Sci, Jiamusi 154007, Peoples R China
3.Univ Otago, Sch Pharm, Dunedin, New Zealand
4.Peking Univ, Dept Pharm, First Hosp, Beijing 100034, Peoples R China

Recommended Citation:
Yang, Yan,Li, Hao,Gao, Kan,et al. Simultaneous quantitation of dexamethasone palmitate and dexamethasone in human plasma by liquid chromatography/tandem mass spectrometry[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2008,862(1-2):119-124.
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