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Identification of the Botanical Source of Stemonae Radix Based on Polymerase Chain Reaction with Specific Primers and Polymerase Chain Reaction-Restriction Fragment Length Polymorphism
Fan, Lan-Lan1; Zhu, Shu2; Chen, Hu-Biao3; Yang, Dong-Hui1; Cai, Shao-Qing1; Komatsu, Katsuko2
关键词Stemona Trnl-trnf Petb-petd Molecular Identification Polymerase Chain Reaction-restriction Fragment Length Polymorphism Stemonae Radix
刊名BIOLOGICAL & PHARMACEUTICAL BULLETIN
2009-09-01
32期:9页:1624-1627
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Pharmacology & Pharmacy
研究领域[WOS]Pharmacology & Pharmacy
关键词[WOS]TUBEROSA ; ALKALOIDS
英文摘要

Stemona sessilifolia, S. japonica and S. tuberosa are the three genuine sources of Stemonae Radix specified in the Chinese Pharmacopoeia (CP) for antitussive and insecticidal remedy. Significant variations in alkaloids composition and content, as well as different degree of antitussive activity Were found among them. In order to accurately identify the genuine sources of Stemonae Radix in the genetic level, two polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) methods were developed based on the sequence differences in chloroplast DNA trnL-trnF and petB-petD regions of the species recorded in CP, as well as S. parviflora and a counterfeit of Stemonae Radix, Asparagus cochinchinensis. By using the restriction enzymes MwoI, AciI and XmnI which were able to recognize specific sequence sites in the trnL-trnF region, and BclI, HincII and BslI which can recognize those in the petB-petD region to digest the corresponding PCR products, the specific digestion pattern enabled the discrimination of the botanical sources of Stemonae Radix effectively and efficiently.

语种英语
WOS记录号WOS:000269349000026
项目编号30425018
资助机构National Science Fund for Distinguished Young Scholars of NSFC ; Ministry of Education, Culture, Sports, Science and Technology of Japan
引用统计
被引频次:8[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/53089
专题北京大学药学院
北京大学药学院_天然药物学系
通讯作者Cai, Shao-Qing
作者单位1.Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China
2.Toyama Univ, Div Parmacognosy, Dept Med Resources, Inst Nat Med, Toyama 9300194, Japan
3.Hong Kong Baptist Univ, Sch Chinese Med, Hong Kong, Hong Kong, Peoples R China
推荐引用方式
GB/T 7714
Fan, Lan-Lan,Zhu, Shu,Chen, Hu-Biao,et al. Identification of the Botanical Source of Stemonae Radix Based on Polymerase Chain Reaction with Specific Primers and Polymerase Chain Reaction-Restriction Fragment Length Polymorphism[J]. BIOLOGICAL & PHARMACEUTICAL BULLETIN,2009,32(9):1624-1627.
APA Fan, Lan-Lan,Zhu, Shu,Chen, Hu-Biao,Yang, Dong-Hui,Cai, Shao-Qing,&Komatsu, Katsuko.(2009).Identification of the Botanical Source of Stemonae Radix Based on Polymerase Chain Reaction with Specific Primers and Polymerase Chain Reaction-Restriction Fragment Length Polymorphism.BIOLOGICAL & PHARMACEUTICAL BULLETIN,32(9),1624-1627.
MLA Fan, Lan-Lan,et al."Identification of the Botanical Source of Stemonae Radix Based on Polymerase Chain Reaction with Specific Primers and Polymerase Chain Reaction-Restriction Fragment Length Polymorphism".BIOLOGICAL & PHARMACEUTICAL BULLETIN 32.9(2009):1624-1627.
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