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学科主题: 药学
题名:
Identification of the Botanical Source of Stemonae Radix Based on Polymerase Chain Reaction with Specific Primers and Polymerase Chain Reaction-Restriction Fragment Length Polymorphism
作者: Fan, Lan-Lan1; Zhu, Shu2; Chen, Hu-Biao3; Yang, Dong-Hui1; Cai, Shao-Qing1; Komatsu, Katsuko2
关键词: Stemona ; trnL-trnF ; petB-petD ; molecular identification ; polymerase chain reaction-restriction fragment length polymorphism ; Stemonae Radix
刊名: BIOLOGICAL & PHARMACEUTICAL BULLETIN
发表日期: 2009-09-01
卷: 32, 期:9, 页:1624-1627
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Pharmacology & Pharmacy
研究领域[WOS]: Pharmacology & Pharmacy
关键词[WOS]: TUBEROSA ; ALKALOIDS
英文摘要:

Stemona sessilifolia, S. japonica and S. tuberosa are the three genuine sources of Stemonae Radix specified in the Chinese Pharmacopoeia (CP) for antitussive and insecticidal remedy. Significant variations in alkaloids composition and content, as well as different degree of antitussive activity Were found among them. In order to accurately identify the genuine sources of Stemonae Radix in the genetic level, two polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) methods were developed based on the sequence differences in chloroplast DNA trnL-trnF and petB-petD regions of the species recorded in CP, as well as S. parviflora and a counterfeit of Stemonae Radix, Asparagus cochinchinensis. By using the restriction enzymes MwoI, AciI and XmnI which were able to recognize specific sequence sites in the trnL-trnF region, and BclI, HincII and BslI which can recognize those in the petB-petD region to digest the corresponding PCR products, the specific digestion pattern enabled the discrimination of the botanical sources of Stemonae Radix effectively and efficiently.

语种: 英语
所属项目编号: 30425018
项目资助者: National Science Fund for Distinguished Young Scholars of NSFC ; Ministry of Education, Culture, Sports, Science and Technology of Japan
WOS记录号: WOS:000269349000026
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/53089
Appears in Collections:北京大学药学院_期刊论文

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作者单位: 1.Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China
2.Toyama Univ, Div Parmacognosy, Dept Med Resources, Inst Nat Med, Toyama 9300194, Japan
3.Hong Kong Baptist Univ, Sch Chinese Med, Hong Kong, Hong Kong, Peoples R China

Recommended Citation:
Fan, Lan-Lan,Zhu, Shu,Chen, Hu-Biao,et al. Identification of the Botanical Source of Stemonae Radix Based on Polymerase Chain Reaction with Specific Primers and Polymerase Chain Reaction-Restriction Fragment Length Polymorphism[J]. BIOLOGICAL & PHARMACEUTICAL BULLETIN,2009,32(9):1624-1627.
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