北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学口腔医学院  > 口腔病理科  > 期刊论文
学科主题: 口腔医学
题名:
Sialoadenitis progression in nonobese diabetic mice and its correlation with expression of apoptosis-associated proteins in salivary glands and serum IgG levels
作者: Qi Ge1; Hua Hong1; Gao Yan1; Lin Qin1; Yu Guang-yan1
关键词: nonobese diabetic (NOD) mice ; sialoadenitis ; apoptosis ; immunoglobulin G
刊名: CHINESE MEDICAL JOURNAL
发表日期: 2007-08-20
卷: 120, 期:16, 页:1426-1431
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medicine, General & Internal
研究领域[WOS]: General & Internal Medicine
关键词[WOS]: PRIMARY SJOGRENS-SYNDROME ; PRONE NOD MICE ; MOUSE MODEL ; AUTOANTIBODIES ; EPITHELIUM ; DISEASE ; CELLS ; FAS
英文摘要:

Background Sjogren syndrome (SS) is an autoimmune disorder characterized by chronic lymphocytic infiltration and decreased secretion in salivary glands. Apoptosis is one of the possible mechanisms involved in acinar epithelial destruction in SS. The role of apoptosis in the initiation and effect phase of sialoadenitis is still controversial. The aim of this study was to observe the roles of apoptosis-associated proteins and serum IgG levels in sialoadenitis progression in nonobese diabetic (NOD) mice.

Methods 2-, 5-, 10-, 15-, 20-week female NOD and matched BALB/c control mice were selected. Saliva and tear flow rate were measured. Serum IgG level was tested by enzyme-linked immunosorbent assay (ELISA). Number of lymphocyte foci (NLF) in submandibular glands (SMGs) was counted under routine hematoxylin/eosin-stained sections. Expression of Fas, Bcl-2 and procaspase3 proteins as well as apoptotic cells in the SMGs were detected by immunohistochemical staining and by terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) assay respectively.

Results Decreased stimulated total flow rate (STFR) and lymphocyte foci in SMGs were first observed in the 10-week NOD group. STFR was negatively correlated with NLF (P<0.05). Serum IgG in NOD mice was significantly higher than that of the control group (P<0.05) and showed a positive correlation with NLF (P<0.05). Fas expression in SMGs acinar cells in NOD mice increased with age and was significantly higher compared with that in the control group. Bcl-2 expression and procaspase3 expression in SMG acinar cells in each NOD group were lower compared with those of the age-matched control mice.

Conclusion Abnormal expression of Fas and Bcl-2 in the SMGs and higher level of serum IgG may contribute to the initiation of sialoadenitis and cause the glandular destruction in NOD mice.

语种: 英语
WOS记录号: WOS:000249134600009
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/53096
Appears in Collections:北京大学口腔医学院_口腔病理科_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Peking Univ, Sch & Hosp Stomatol, Dept Oral & Maxillofacial Surg, Beijing 100081, Peoples R China
2.Peking Univ, Sch & Hosp Stomatol, Dept Tradit Chinese Med & Oral Med, Beijing 100081, Peoples R China
3.Peking Univ, Sch & Hosp Stomatol, Dept Pathol, Beijing 100081, Peoples R China
4.Peking Univ, Sch & Hosp Stomatol, Dept Lab Examinat, Beijing 100081, Peoples R China

Recommended Citation:
Qi Ge,Hua Hong,Gao Yan,et al. Sialoadenitis progression in nonobese diabetic mice and its correlation with expression of apoptosis-associated proteins in salivary glands and serum IgG levels[J]. CHINESE MEDICAL JOURNAL,2007,120(16):1426-1431.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Qi Ge]'s Articles
[Hua Hong]'s Articles
[Gao Yan]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Qi Ge]‘s Articles
[Hua Hong]‘s Articles
[Gao Yan]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace