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学科主题口腔医学
Sialoadenitis progression in nonobese diabetic mice and its correlation with expression of apoptosis-associated proteins in salivary glands and serum IgG levels
Qi Ge1; Hua Hong1; Gao Yan1; Lin Qin1; Yu Guang-yan1
关键词Nonobese Diabetic (Nod) Mice Sialoadenitis Apoptosis Immunoglobulin g
刊名CHINESE MEDICAL JOURNAL
2007-08-20
120期:16页:1426-1431
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Medicine, General & Internal
研究领域[WOS]General & Internal Medicine
关键词[WOS]PRIMARY SJOGRENS-SYNDROME ; PRONE NOD MICE ; MOUSE MODEL ; AUTOANTIBODIES ; EPITHELIUM ; DISEASE ; CELLS ; FAS
英文摘要

Background Sjogren syndrome (SS) is an autoimmune disorder characterized by chronic lymphocytic infiltration and decreased secretion in salivary glands. Apoptosis is one of the possible mechanisms involved in acinar epithelial destruction in SS. The role of apoptosis in the initiation and effect phase of sialoadenitis is still controversial. The aim of this study was to observe the roles of apoptosis-associated proteins and serum IgG levels in sialoadenitis progression in nonobese diabetic (NOD) mice.

Methods 2-, 5-, 10-, 15-, 20-week female NOD and matched BALB/c control mice were selected. Saliva and tear flow rate were measured. Serum IgG level was tested by enzyme-linked immunosorbent assay (ELISA). Number of lymphocyte foci (NLF) in submandibular glands (SMGs) was counted under routine hematoxylin/eosin-stained sections. Expression of Fas, Bcl-2 and procaspase3 proteins as well as apoptotic cells in the SMGs were detected by immunohistochemical staining and by terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) assay respectively.

Results Decreased stimulated total flow rate (STFR) and lymphocyte foci in SMGs were first observed in the 10-week NOD group. STFR was negatively correlated with NLF (P<0.05). Serum IgG in NOD mice was significantly higher than that of the control group (P<0.05) and showed a positive correlation with NLF (P<0.05). Fas expression in SMGs acinar cells in NOD mice increased with age and was significantly higher compared with that in the control group. Bcl-2 expression and procaspase3 expression in SMG acinar cells in each NOD group were lower compared with those of the age-matched control mice.

Conclusion Abnormal expression of Fas and Bcl-2 in the SMGs and higher level of serum IgG may contribute to the initiation of sialoadenitis and cause the glandular destruction in NOD mice.

语种英语
WOS记录号WOS:000249134600009
Citation statistics
Cited Times:3[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/53096
Collection北京大学口腔医学院_口腔病理科
北京大学第二临床医学院_感染科
北京大学口腔医学院_口腔黏膜科
北京大学口腔医学院_检验科
北京大学口腔医学院_外科
作者单位1.Peking Univ, Sch & Hosp Stomatol, Dept Oral & Maxillofacial Surg, Beijing 100081, Peoples R China
2.Peking Univ, Sch & Hosp Stomatol, Dept Tradit Chinese Med & Oral Med, Beijing 100081, Peoples R China
3.Peking Univ, Sch & Hosp Stomatol, Dept Pathol, Beijing 100081, Peoples R China
4.Peking Univ, Sch & Hosp Stomatol, Dept Lab Examinat, Beijing 100081, Peoples R China
Recommended Citation
GB/T 7714
Qi Ge,Hua Hong,Gao Yan,et al. Sialoadenitis progression in nonobese diabetic mice and its correlation with expression of apoptosis-associated proteins in salivary glands and serum IgG levels[J]. CHINESE MEDICAL JOURNAL,2007,120(16):1426-1431.
APA Qi Ge,Hua Hong,Gao Yan,Lin Qin,&Yu Guang-yan.(2007).Sialoadenitis progression in nonobese diabetic mice and its correlation with expression of apoptosis-associated proteins in salivary glands and serum IgG levels.CHINESE MEDICAL JOURNAL,120(16),1426-1431.
MLA Qi Ge,et al."Sialoadenitis progression in nonobese diabetic mice and its correlation with expression of apoptosis-associated proteins in salivary glands and serum IgG levels".CHINESE MEDICAL JOURNAL 120.16(2007):1426-1431.
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