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Comprehensive two-dimensional gas chromatography, retention indices and time-of-flight mass spectra of flavonoids and chalcones
Gao, Xin1,2; Williams, Spencer J.3,4; Woodman, Owen L.5; Marriott, Philip J.1
关键词Flavonoids Gc/ms Comprehensive Two-dimensional Gas Chromatography (Gc x Gc) Time-of-flight Mass Spectrometry (Tofms) Retention Indices Mass Spectrometry Database
刊名JOURNAL OF CHROMATOGRAPHY A
2010-12-24
DOI10.1016/j.chroma.2010.10.093
1217期:52页:8317-8326
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]TRIMETHYLSILYL DERIVATIVES ; LC-DAD ; LIQUID-CHROMATOGRAPHY ; FLAVANONE AGLYCONES ; PHENOLIC-COMPOUNDS ; AMINO-ACID ; SPECTROMETRY ; SAMPLES ; IDENTIFICATION ; QUANTIFICATION
英文摘要

The applicability of comprehensive two-dimensional gas chromatography (GC x GC) for flavonoids analysis was investigated by separation and identification of flavonoids in standards, and a complex matrix natural sample. The modulation temperature was optimized to achieve the best separation and signal enhancement. The separation pattern of trimethylsilyl (TMS) derivatives of flavonoids was compared on two complementary column sets. Whilst the BPX5/BPX50 (NP/P) column set offers better overall separation, BPX50/BPX5 (P/NP) provides better peak shape and sensitivity. Comparison of the identification power of GC x GC-TOFMS against both the NIST05 MS library and a laboratory (created in-house) TOFMS library was carried out on a flavonoid mixture. The basic retention index information on high-performance capillary columns with a non-polar stationary phase was established and database of mass spectra of trimethylsilyl derivatives of flavonoids was compiled. TOFMS coupled to GC x GC enabled satisfactory identification of flavonoids in complex matrix samples at their LOD over a range of 0.5-10 mu g/mL.. Detection of all compounds was based on full-scan mass spectra and for each compound a characteristic ion was chosen for further quantification. This study shows that GC x GC-TOFMS yields high specificity for flavonoids derived from real natural samples, dark chocolate, propolis, and chrysanthemum. (C) 2010 Elsevier B.V. All rights reserved.

语种英语
WOS记录号WOS:000285899400028
引用统计
被引频次:17[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/53097
专题北京大学药学院
作者单位1.Peking Univ, Dept Pharm, Beijing 100191, Peoples R China
2.Univ Melbourne, Sch Chem, Melbourne, Vic 3010, Australia
3.RMIT Univ, Sch Appl Sci, Australian Ctr Res Separat Sci, Melbourne, Vic 3001, Australia
4.Univ Melbourne, Mol Sci & Biotechnol Inst Bio21, Melbourne, Vic 3010, Australia
5.RMIT Univ, Sch Med Sci, Bundoora, Vic 3083, Australia
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GB/T 7714
Gao, Xin,Williams, Spencer J.,Woodman, Owen L.,et al. Comprehensive two-dimensional gas chromatography, retention indices and time-of-flight mass spectra of flavonoids and chalcones[J]. JOURNAL OF CHROMATOGRAPHY A,2010,1217(52):8317-8326.
APA Gao, Xin,Williams, Spencer J.,Woodman, Owen L.,&Marriott, Philip J..(2010).Comprehensive two-dimensional gas chromatography, retention indices and time-of-flight mass spectra of flavonoids and chalcones.JOURNAL OF CHROMATOGRAPHY A,1217(52),8317-8326.
MLA Gao, Xin,et al."Comprehensive two-dimensional gas chromatography, retention indices and time-of-flight mass spectra of flavonoids and chalcones".JOURNAL OF CHROMATOGRAPHY A 1217.52(2010):8317-8326.
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