IR@PKUHSC  > 北京大学第三临床医学院
学科主题临床医学
Ginsenoside Rb1 Protects Neonatal Rat Cardiomyocytes from Hypoxia/Ischemia Induced Apoptosis and Inhibits Activation of the Mitochondrial Apoptotic Pathway
Yan, Xu1,2; Tian, Jinwen3; Wu, Hongjin1; Liu, Yuna4; Ren, Jianxun4; Zheng, Sidao4; Zhang, Chengying4; Yang, Cui4; Li, Yang3; Wang, Shengqi5
刊名EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE
2014
DOI10.1155/2014/149195
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Integrative & Complementary Medicine
研究领域[WOS]Integrative & Complementary Medicine
关键词[WOS]INTESTINAL ISCHEMIA-REPERFUSION ; PERMEABILITY TRANSITION ; HEART-DISEASE ; CELL-DEATH ; INJURY ; EXTRACT ; UPDATE ; LIVER ; MICE
英文摘要

Aim. To investigate the effect of Ginsenoside Rb1 (GS-Rb1) on hypoxia/ischemia (H/I) injury in cardiomyocytes in vitro and the mitochondrial apoptotic pathway mediated mechanism. Methods. Neonatal rat cardiomyocytes (NRCMs) for the H/I groups were kept in DMEM without glucose and serum, and were placed into a hypoxic jar for 24 h. GS-Rb1 at concentrations from 2.5 to 40 mu M was given during hypoxic period for 24 h. NRCMs injury was determined by MTT and lactate dehydrogenase (LDH) leakage assay. Cell apoptosis, ROS accumulation, and mitochondrial membrane potential (MMP) were assessed by flow cytometry. Cytosolic translocation of mitochondrial cytochrome c and Bcl-2 family proteins were determined by Western blot. Caspase-3 and caspase-9 activities were determined by the assay kit. Results. GS-Rb1 significantly reduced cell death and LDH leakage induced by H/I. It also reduced H/I induced NRCMs apoptosis induced by H/I, in accordance with a minimal reactive oxygen species (ROS) burst. Moreover, GS-Rb1 markedly decreased the translocation of cytochrome c from the mitochondria to the cytosol, increased the Bcl-2/Bax ratio, and preserved mitochondrial transmembrane potential (Delta Psi m). Its administration also inhibited activities of caspase-9 and caspase-3. Conclusion. Administration of GS-Rb1 during H/I in vitro is involved in cardioprotection by inhibiting apoptosis, which may be due to inhibition of the mitochondrial apoptotic pathway.

语种英语
WOS记录号WOS:000339199600001
项目编号81273890 ; 81030002/H02 ; 81173367 ; 2013ZZ-57
资助机构National Natural Science Foundation of China ; Beijing Postdoctoral Research Foundation
引用统计
被引频次:6[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/53104
专题北京大学第三临床医学院
作者单位1.Peking Univ, Hosp 3, Haidian Sect, Beijing Haidian Hosp, Beijing 100080, Peoples R China
2.Postdoctoral Workstn Zhongguancun Haidian Sci Pk, Beijing 100195, Peoples R China
3.Gen Hosp Peoples Liberat Army, Inst Geriatr Cardiol, Beijing 100853, Peoples R China
4.Beijing Hosp Integrated Tradit Chinese & Western, Beijing 100039, Peoples R China
5.Beijing Inst Radiat Med, Beijing 100850, Peoples R China
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GB/T 7714
Yan, Xu,Tian, Jinwen,Wu, Hongjin,et al. Ginsenoside Rb1 Protects Neonatal Rat Cardiomyocytes from Hypoxia/Ischemia Induced Apoptosis and Inhibits Activation of the Mitochondrial Apoptotic Pathway[J]. EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE,2014.
APA Yan, Xu.,Tian, Jinwen.,Wu, Hongjin.,Liu, Yuna.,Ren, Jianxun.,...&Wang, Shengqi.(2014).Ginsenoside Rb1 Protects Neonatal Rat Cardiomyocytes from Hypoxia/Ischemia Induced Apoptosis and Inhibits Activation of the Mitochondrial Apoptotic Pathway.EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE.
MLA Yan, Xu,et al."Ginsenoside Rb1 Protects Neonatal Rat Cardiomyocytes from Hypoxia/Ischemia Induced Apoptosis and Inhibits Activation of the Mitochondrial Apoptotic Pathway".EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE (2014).
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