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学科主题: 临床医学
题名:
Ginsenoside Rb1 Protects Neonatal Rat Cardiomyocytes from Hypoxia/Ischemia Induced Apoptosis and Inhibits Activation of the Mitochondrial Apoptotic Pathway
作者: Yan, Xu1,2; Tian, Jinwen3; Wu, Hongjin1; Liu, Yuna4; Ren, Jianxun4; Zheng, Sidao4; Zhang, Chengying4; Yang, Cui4; Li, Yang3; Wang, Shengqi5
刊名: EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE
发表日期: 2014
DOI: 10.1155/2014/149195
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Integrative & Complementary Medicine
研究领域[WOS]: Integrative & Complementary Medicine
关键词[WOS]: INTESTINAL ISCHEMIA-REPERFUSION ; PERMEABILITY TRANSITION ; HEART-DISEASE ; CELL-DEATH ; INJURY ; EXTRACT ; UPDATE ; LIVER ; MICE
英文摘要:

Aim. To investigate the effect of Ginsenoside Rb1 (GS-Rb1) on hypoxia/ischemia (H/I) injury in cardiomyocytes in vitro and the mitochondrial apoptotic pathway mediated mechanism. Methods. Neonatal rat cardiomyocytes (NRCMs) for the H/I groups were kept in DMEM without glucose and serum, and were placed into a hypoxic jar for 24 h. GS-Rb1 at concentrations from 2.5 to 40 mu M was given during hypoxic period for 24 h. NRCMs injury was determined by MTT and lactate dehydrogenase (LDH) leakage assay. Cell apoptosis, ROS accumulation, and mitochondrial membrane potential (MMP) were assessed by flow cytometry. Cytosolic translocation of mitochondrial cytochrome c and Bcl-2 family proteins were determined by Western blot. Caspase-3 and caspase-9 activities were determined by the assay kit. Results. GS-Rb1 significantly reduced cell death and LDH leakage induced by H/I. It also reduced H/I induced NRCMs apoptosis induced by H/I, in accordance with a minimal reactive oxygen species (ROS) burst. Moreover, GS-Rb1 markedly decreased the translocation of cytochrome c from the mitochondria to the cytosol, increased the Bcl-2/Bax ratio, and preserved mitochondrial transmembrane potential (Delta Psi m). Its administration also inhibited activities of caspase-9 and caspase-3. Conclusion. Administration of GS-Rb1 during H/I in vitro is involved in cardioprotection by inhibiting apoptosis, which may be due to inhibition of the mitochondrial apoptotic pathway.

语种: 英语
所属项目编号: 81273890 ; 81030002/H02 ; 81173367 ; 2013ZZ-57
项目资助者: National Natural Science Foundation of China ; Beijing Postdoctoral Research Foundation
WOS记录号: WOS:000339199600001
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/53104
Appears in Collections:北京大学第三临床医学院_期刊论文

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作者单位: 1.Peking Univ, Hosp 3, Haidian Sect, Beijing Haidian Hosp, Beijing 100080, Peoples R China
2.Postdoctoral Workstn Zhongguancun Haidian Sci Pk, Beijing 100195, Peoples R China
3.Gen Hosp Peoples Liberat Army, Inst Geriatr Cardiol, Beijing 100853, Peoples R China
4.Beijing Hosp Integrated Tradit Chinese & Western, Beijing 100039, Peoples R China
5.Beijing Inst Radiat Med, Beijing 100850, Peoples R China

Recommended Citation:
Yan, Xu,Tian, Jinwen,Wu, Hongjin,et al. Ginsenoside Rb1 Protects Neonatal Rat Cardiomyocytes from Hypoxia/Ischemia Induced Apoptosis and Inhibits Activation of the Mitochondrial Apoptotic Pathway[J]. EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE,2014.
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