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学科主题临床医学
Dominant expression of 85-kDa form of cortactin in colorectal cancer
Zhang, LH1; Tian, B1; Diao, LR1; Xiong, YY1; Tian, SF1; Zhang, BH1; Li, WM1; Ren, H1; Li, Y1; Ji, JF1
关键词Colorectal Neoplasms Cortactin Gene Expression Cell Movement
刊名JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
2006-02-01
DOI10.1007/s00432-005-0046-8
132期:2页:113-120
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology
研究领域[WOS]Oncology
关键词[WOS]CHROMOSOME 11Q13 AMPLIFICATION ; SQUAMOUS-CELL CARCINOMA ; BREAST-CANCER ; TYROSINE PHOSPHORYLATION ; POOR-PROGNOSIS ; ACTIN ; OVEREXPRESSION ; CYTOSKELETON ; COMPLEX ; SITES
英文摘要

Purpose: Cortactin is commonly expressed in several human cancers, which may alter their invasive or metastatic properties. Eighty five kilodalton form (p85) and 80-kDa form (p80) of cortactin are two separate bands in SDS-PAGE representing different conformational states. The objective of this study was to investigate cortactin expression in colorectal cancer (CRC). Experimental Design: Cortactin expression was studied in an eight paired laser capture microdissection (LCM) CRC tissues and matched non-cancerous epithelia by immunoblotting. The expression in 58 CRC and two cell lines, HCT8 and HCT116, was studied respectively by immunohistochemistry and confocal laser scanning immunofluorescence. Results: Dominant expression of p85 was identified in LCM-procured CRC tissues compared with equal intensity of p85 and p80 forms in non-cancerous tissues, while the amount of total cortactin was approximate. Immunohistochemistry analysis demonstrated that cortactin located in the cytoplasm of tumor cells and adjacent non-cancerous cells, and its expression was negatively correlated with TNM staging and lymphatic invasion status. However, the invasion fronts in 3 of 58 primary tumors and 28 of 39 available lymph node metastases were intensively stained. Further, immunofluorescence analysis showed that cortactin was distributed in cytoplasm and enriched in the front of the extending lamellipodia at adhering side of cultured cancer cells. Conclusions: Our results demonstrated the dominant expression of p85 form of cortactin in CRC for the first time. The enrichment of cortactin in the invasion front of some tumor cells and in the extending lamellipodia of cultured cancer cells suggests that cortactin may help cancer cell movement.

语种英语
WOS记录号WOS:000233737400007
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被引频次:17[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/53108
专题北京大学临床肿瘤学院
北京大学第二临床医学院_血液科
北京大学临床肿瘤学院_胃肠肿瘤中心一病区
北京大学临床肿瘤学院_高技术实验室
作者单位1.Peking Univ, Sch Oncol, Beijing Inst Canc Res, Beijing Canc Hosp, Beijing 100034, Peoples R China
2.Fudan Univ, Zhongshan Hosp, Liver Canc Inst, Shanghai 200433, Peoples R China
3.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Shanghai, Peoples R China
4.Wuhan Univ, Zhongnan Hosp, Inst Oncol, Wuhan 430072, Peoples R China
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Zhang, LH,Tian, B,Diao, LR,et al. Dominant expression of 85-kDa form of cortactin in colorectal cancer[J]. JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY,2006,132(2):113-120.
APA Zhang, LH.,Tian, B.,Diao, LR.,Xiong, YY.,Tian, SF.,...&Ji, JF.(2006).Dominant expression of 85-kDa form of cortactin in colorectal cancer.JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY,132(2),113-120.
MLA Zhang, LH,et al."Dominant expression of 85-kDa form of cortactin in colorectal cancer".JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY 132.2(2006):113-120.
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