北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学临床肿瘤学院  > 期刊论文
学科主题: 临床医学
题名:
Dominant expression of 85-kDa form of cortactin in colorectal cancer
作者: Zhang, LH1; Tian, B1; Diao, LR1; Xiong, YY1; Tian, SF1; Zhang, BH1; Li, WM1; Ren, H1; Li, Y1; Ji, JF1
关键词: colorectal neoplasms ; cortactin ; gene expression ; cell movement
刊名: JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
发表日期: 2006-02-01
DOI: 10.1007/s00432-005-0046-8
卷: 132, 期:2, 页:113-120
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology
研究领域[WOS]: Oncology
关键词[WOS]: CHROMOSOME 11Q13 AMPLIFICATION ; SQUAMOUS-CELL CARCINOMA ; BREAST-CANCER ; TYROSINE PHOSPHORYLATION ; POOR-PROGNOSIS ; ACTIN ; OVEREXPRESSION ; CYTOSKELETON ; COMPLEX ; SITES
英文摘要:

Purpose: Cortactin is commonly expressed in several human cancers, which may alter their invasive or metastatic properties. Eighty five kilodalton form (p85) and 80-kDa form (p80) of cortactin are two separate bands in SDS-PAGE representing different conformational states. The objective of this study was to investigate cortactin expression in colorectal cancer (CRC). Experimental Design: Cortactin expression was studied in an eight paired laser capture microdissection (LCM) CRC tissues and matched non-cancerous epithelia by immunoblotting. The expression in 58 CRC and two cell lines, HCT8 and HCT116, was studied respectively by immunohistochemistry and confocal laser scanning immunofluorescence. Results: Dominant expression of p85 was identified in LCM-procured CRC tissues compared with equal intensity of p85 and p80 forms in non-cancerous tissues, while the amount of total cortactin was approximate. Immunohistochemistry analysis demonstrated that cortactin located in the cytoplasm of tumor cells and adjacent non-cancerous cells, and its expression was negatively correlated with TNM staging and lymphatic invasion status. However, the invasion fronts in 3 of 58 primary tumors and 28 of 39 available lymph node metastases were intensively stained. Further, immunofluorescence analysis showed that cortactin was distributed in cytoplasm and enriched in the front of the extending lamellipodia at adhering side of cultured cancer cells. Conclusions: Our results demonstrated the dominant expression of p85 form of cortactin in CRC for the first time. The enrichment of cortactin in the invasion front of some tumor cells and in the extending lamellipodia of cultured cancer cells suggests that cortactin may help cancer cell movement.

语种: 英语
WOS记录号: WOS:000233737400007
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/53108
Appears in Collections:北京大学临床肿瘤学院_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Peking Univ, Sch Oncol, Beijing Inst Canc Res, Beijing Canc Hosp, Beijing 100034, Peoples R China
2.Fudan Univ, Zhongshan Hosp, Liver Canc Inst, Shanghai 200433, Peoples R China
3.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Shanghai, Peoples R China
4.Wuhan Univ, Zhongnan Hosp, Inst Oncol, Wuhan 430072, Peoples R China

Recommended Citation:
Zhang, LH,Tian, B,Diao, LR,et al. Dominant expression of 85-kDa form of cortactin in colorectal cancer[J]. JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY,2006,132(2):113-120.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Zhang, LH]'s Articles
[Tian, B]'s Articles
[Diao, LR]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Zhang, LH]‘s Articles
[Tian, B]‘s Articles
[Diao, LR]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace