IR@PKUHSC  > 北京大学第二临床医学院  > 北京大学肝病研究所
学科主题临床医学
Performance evaluation and comparison of the newly developed Elecsys anti-HCV II assay with other widely used assays
Yang, Ruifeng; Guan, Wenli; Wang, Qian; Liu, Yan; Wei, Lai
关键词Hepatitis c Virus Anti-hcv Elecsys Anti-hcv Ii Architect Anti-hcv Vitros Anti-hcv Genotype
刊名CLINICA CHIMICA ACTA
2013-11-15
DOI10.1016/j.cca.2013.09.010
426页:95-101
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Medical Laboratory Technology
资助者National Science and Technology Major Project for Infectious Diseases Control ; Peking University People&prime ; s Hospital Research and Development Funds ; National Science and Technology Major Project for Infectious Diseases Control ; Peking University People&prime ; s Hospital Research and Development Funds
研究领域[WOS]Medical Laboratory Technology
关键词[WOS]HEPATITIS-C VIRUS ; RECOMBINANT IMMUNOBLOT ASSAY ; HUMAN-IMMUNODEFICIENCY-VIRUS ; INFECTION ; GENOTYPES ; 2ND-GENERATION ; PREVALENCE ; CHINA
英文摘要

Background: Anti-HCV assays remain the first choice for screening HCV infection in most clinical laboratories. The Elecsys anti-HCV II assay has been recently launched and we aimed to evaluate its performance compared with other widely used methods.

Methods: Four seroconversion panels, 861 consecutive sera under routine clinical conditions, 100 preselected sera with low positive anti-HCV results and 178 samples from patients infected with HIV were tested using Elecsys anti-HCV II, Architect anti-HCV and Vitros anti-HCV assays. Confirmatory testing was performed using RIBA and HCV RNA tests. Moreover, 203 samples with different HCV genotypes were assessed using Elecsys anti-HCV H.

Results: Elecsys anti-HCV II detected seroconversion 7-14 days earlier than the Architect and Vitros assays. Furthermore, it had 100% sensitivity and superior specificity in screening routine clinical samples, including those with low positive anti-HCV, and detected 97.6% (122/125) anti-HCV-positive samples from HIV-infected patients with HCV viremia. However, the anti-HCV levels in the genotype 3b samples were slightly underestimated.

Conclusions: Elecsys anti-HCV II shortens the seroconversion window. It is suitable for screening HCV infection in clinical samples, including those from immunocompromised patients, due to the excellent sensitivity and specificity. Further investigation of the subtype inclusivity in a larger sample number might be warranted. (C) 2013 Elsevier B.V. All rights reserved.

语种英语
所属项目编号2008ZX10002-012 ; 2008ZX10002-013 ; 2012ZX10002003 ; RDC2012-06
资助者National Science and Technology Major Project for Infectious Diseases Control ; Peking University People&prime ; s Hospital Research and Development Funds ; National Science and Technology Major Project for Infectious Diseases Control ; Peking University People&prime ; s Hospital Research and Development Funds
WOS记录号WOS:000328809000018
Citation statistics
Cited Times:14[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/53131
Collection北京大学第二临床医学院_北京大学肝病研究所
作者单位Peking Univ, Peoples Hosp, Inst Hepatol, Beijing Key Lab Hepatitis & Immunotherapy Liver D, Beijing 100044, Peoples R China
Recommended Citation
GB/T 7714
Yang, Ruifeng,Guan, Wenli,Wang, Qian,et al. Performance evaluation and comparison of the newly developed Elecsys anti-HCV II assay with other widely used assays[J]. CLINICA CHIMICA ACTA,2013,426:95-101.
APA Yang, Ruifeng,Guan, Wenli,Wang, Qian,Liu, Yan,&Wei, Lai.(2013).Performance evaluation and comparison of the newly developed Elecsys anti-HCV II assay with other widely used assays.CLINICA CHIMICA ACTA,426,95-101.
MLA Yang, Ruifeng,et al."Performance evaluation and comparison of the newly developed Elecsys anti-HCV II assay with other widely used assays".CLINICA CHIMICA ACTA 426(2013):95-101.
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