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学科主题: 药学
题名:
Chemical analysis of Eriocaulon buergerianum and adulterating species by high-performance liquid chromatography with diode array detection and electrospray ionization tandem mass spectrometry
作者: Qiao, Xue1; Ye, Guan2; Liu, Chun-Fang1; Zhang, Zhi-Xiang3; Tu, Qiang3; Dong, Jing4; Li, Yue-Qi4; Guo, De-An1; Ye, Min1
关键词: Eriocaulon buergerianum ; HPLC-DAD-ESI-MS(n) ; Flavonoids ; Naphthopyranones ; Chinese herbal medicine
刊名: JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
发表日期: 2012-01-05
DOI: 10.1016/j.jpba.2011.08.033
卷: 57, 页:133-142
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Chemistry, Analytical ; Pharmacology & Pharmacy
研究领域[WOS]: Chemistry ; Pharmacology & Pharmacy
关键词[WOS]: PHENOLIC-COMPOUNDS ; FLAVONOIDS ; PATULETIN ; LIGULATUM
英文摘要:

Eriocaulon buergerianum Koern. is the botanical source for the Chinese herbal medicine Gu-Jing-Cao. Other Eriocaulon species are also used as the same herb in local areas and are difficult to be differentiated. In order to improve the quality control of Gu-Jing-Cao, chemical constituents of E. buergerianum and adulterating species were analyzed by high-performance liquid chromatography with diode array detection and electrospray ionization tandem mass spectrometry (HPLC-DAD-ESI-MS(n)). The 70% methanol extracts were separated on a Zorbax SB-C(18) column and eluted with acetonitrile-water (each containing 0.1% formic acid). The compounds were identified by ion-trap mass spectrometry in both positive and negative ion modes. From E. buergerianum, E. faberi, E. sexangulare and E. cinereum, a total of 72 compounds were characterized, including 37 flavonols, 6 flavones, 4 isoflavones, 6 xanthones, 14 naphthopyranones, 3 phenolic acids, and 2 other flavonoids. Chemical variation of these four species were studied at three tiers, HPLC fingerprinting, quantitation of six major flavonoids, and semi-quantitative analysis of all characterized compounds, in combination with principal component analysis. E. buergerianum contained abundant flavonols and naphthopyranones, with minor flavones and xanthones; E. cinereum contained abundant isoflavones and flavones, together with few naphthopyranones: E. sexangulare was rich in flavones; and E. faberi contained abundant xanthones. Based on the above chemical analysis, E. buergerianum could be explicitly differentiated from the adulterating species, and the botanical species of 13 commercial batches of Gu-Jing-Cao were identified correctly. This is the first report on comprehensive chemical analysis of Eriocaulon species. (C) 2011 Elsevier B.V. All rights reserved.

语种: 英语
所属项目编号: 985-2-119-121
项目资助者: Peking University Health Science Center
WOS记录号: WOS:000297539500020
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/53136
Appears in Collections:北京大学药学院_期刊论文

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作者单位: 1.Shanghai Pharmaceut Grp, Cent Res Inst, Shanghai 201203, Peoples R China
2.Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China
3.Beijing Forestry Univ, Coll Biol Sci & Biotechnol, Beijing 100083, Peoples R China
4.Shimadzu Int Trading Shanghai Co Ltd, Beijing Off, Beijing 100020, Peoples R China

Recommended Citation:
Qiao, Xue,Ye, Guan,Liu, Chun-Fang,et al. Chemical analysis of Eriocaulon buergerianum and adulterating species by high-performance liquid chromatography with diode array detection and electrospray ionization tandem mass spectrometry[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2012,57:133-142.
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