|Expression of midkine and its clinical significance in esophageal squamous cell carcinoma|
|Ren, Ying-Jia1; Zhang, Qing-Yun1|
|关键词||Midkine Esophageal Squamous Cell Carcinoma Expression|
|刊名||WORLD JOURNAL OF GASTROENTEROLOGY|
|WOS标题词||Science & Technology|
|类目[WOS]||Gastroenterology & Hepatology|
|研究领域[WOS]||Gastroenterology & Hepatology|
|关键词[WOS]||SITU HYBRIDIZATION ANALYSES ; ACID RESPONSIVE GENE ; MOUSE EMBRYOGENESIS ; BLADDER-CANCER ; THERAPY ; OVEREXPRESSION ; PROGRESSION ; MARKER ; TUMORS ; MK|
Aim: To investigate the expression of midkine in esophageal squamous cell carcinoma (ESCC) and analyze its relationship with clinicopathological features.
Methods: RT-PCR and immunocytochemical staining were used to detect the expression of midkine mRNA and protein in EC109 cells, respectively. Then the expression of midkine in 66 cases of ESCC samples were detected by immunohistochemistry using monoclonal antibodies against human midkine.
Results: Midkine was expressed in EC109 cell by RTPCR and immunocytochemistry. The immunoreactivity was detected in 56.1% (37/66) of the ESCC samples. The expression of midkine was found in cytoplasm of tumor cells. Notably, the intensity of midkine was stronger at the area abundant in vessels and the invading border of the tumors. Midkine was more intensely expressed in well differentiated tumors (76.9%) than in moderately and poorly differentiated tumors (43.1% and 41.2%, respectively) (P<0.05). There was no statistically significant correlation between midkine expression and gender, age, clinical stage, lymph node metastasis or survival in ESCC.
Conclusion: Midkine is overexpressed in ESCC. It may play a role in tumor angiogenesis and invasion. The expression of midkine is correlated with tumor cell differentiation in ESCC. The more poorly tumor cells differentiate, the weaker midkine expresses. (C) 2006 The WJG Press. All rights reserved.
|作者单位||1.Beijing Canc Hosp, Dept Lab Med, Beijing 100036, Peoples R China|
2.Peking Univ, Sch Oncol, Dept Immunol, Beijing Inst Canc Res, Beijing 100036, Peoples R China
|Ren, Ying-Jia,Zhang, Qing-Yun. Expression of midkine and its clinical significance in esophageal squamous cell carcinoma[J]. WORLD JOURNAL OF GASTROENTEROLOGY,2006,12(13):2006-2010.|
|APA||Ren, Ying-Jia,&Zhang, Qing-Yun.(2006).Expression of midkine and its clinical significance in esophageal squamous cell carcinoma.WORLD JOURNAL OF GASTROENTEROLOGY,12(13),2006-2010.|
|MLA||Ren, Ying-Jia,et al."Expression of midkine and its clinical significance in esophageal squamous cell carcinoma".WORLD JOURNAL OF GASTROENTEROLOGY 12.13(2006):2006-2010.|