|Cytotoxic effects of T cells induced by fusion protein 6B11-pulsed dendritic cells on ovarian carcinoma cells|
|Yang, Wenlan1; Feng, Jie1; Chang, Xiaohong1; Fu, Tianyun1; Ye, Xue1; Zhang, Hong1; Li, Xiaoping1; Wen, Hongwu1; Feng, Limin1; Tong, Chunrong1; Cui, Heng1|
|关键词||t Cells Fusion Protein 6b11 Dendritic Cells Ovarian Carcinorna Cells Anti-tumor Immunotherapy|
|WOS标题词||Science & Technology|
|类目[WOS]||Oncology ; Obstetrics & Gynecology|
|研究领域[WOS]||Oncology ; Obstetrics & Gynecology|
|关键词[WOS]||CARCINOEMBRYONIC ANTIGEN CEA ; CANCER-IMMUNOTHERAPY ; ANTITUMOR IMMUNITY ; PEPTIDE ; VACCINATION ; SYSTEM|
Introduction. 6B11 anti-idiotype minibody, a fusion protein, has been shown to mimic ovarian carcinoma associated antigen OC166-9. This study was designed to determine whether 6B11 anti-idiotype minibody-pulsed dendritic cells (DCs) can induce cytotoxic T cells against ovarian cancer cells.
Methods. Monocytes were isolated from peripheral blood mononuclear cells collected from patients with epithelial ovarian carcinoma (n=10). The monocytes-derived immature DCs were stimulated by cytokines, and mature DCs were pulsed with 6B11 anti-idiotype-minibody or murine F(ab)′(2) fragments. The proliferation of autologous T cells induced by DCs was determined by H-3-thymidine uptake. The cytotoxicity of DC-activated T cells against autologous carcinoma cells was determined by Cr-51-release assay.
Results. Purified T cells demonstrated strong proliferation following incubation with 6B11 anti-idiotype minibody-pulsed DCs in 4 of 10 patients. The specific cytotoxicity of purified T cells against autologous carcinoma cells was induced after stimulation with 6B11 anti-idiotype minibody-pulsed DCs in 5 of 10 patients with cytotoxic effects ranging from 25 to 95%. In contrast, isotype murine F(ab)′(2) fragments-pulsed DCs did not induce T cell proliferation and cytotoxicity against the targets. Additionally, the cytotoxic effect was partially inhibited by anti-MHC class-I antibody indicating that the cytotoxic effects are antigen-specific.
Conclusion. 6B11 anti-idiotype-antibody-pulsed DCs can induce T cell proliferation and T cell-mediated cytotoxicity against autologous ovarian tumor cells in vitro. The cytotoxic effects of T cells against autologous tumor cells are antigen-specific. These data implicate the rationale for the use of 6B11 anti-idiotype minibody as immunotherapy against ovarian carcinoma. (c) 2007 Elsevier Inc. All rights reserved.
|作者单位||1.Tian Tan Hosp, Dept Gynecol, Beijing 100050, Peoples R China|
2.Peking Univ, Peoples Hosp, Gynecol Oncol Ctr, Beijing 100044, Peoples R China
3.Peking Univ, Affiliated Hosp 1, Dept Gynecol, Beijing 100044, Peoples R China
4.Peking Univ, Peoples Hosp, Dept Hematol, Beijing 100044, Peoples R China
|Yang, Wenlan,Feng, Jie,Chang, Xiaohong,et al. Cytotoxic effects of T cells induced by fusion protein 6B11-pulsed dendritic cells on ovarian carcinoma cells[J]. GYNECOLOGIC ONCOLOGY,2007,105(1):238-243.|
|APA||Yang, Wenlan.,Feng, Jie.,Chang, Xiaohong.,Fu, Tianyun.,Ye, Xue.,...&Cui, Heng.(2007).Cytotoxic effects of T cells induced by fusion protein 6B11-pulsed dendritic cells on ovarian carcinoma cells.GYNECOLOGIC ONCOLOGY,105(1),238-243.|
|MLA||Yang, Wenlan,et al."Cytotoxic effects of T cells induced by fusion protein 6B11-pulsed dendritic cells on ovarian carcinoma cells".GYNECOLOGIC ONCOLOGY 105.1(2007):238-243.|