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Immunohistochemical detection of the BRAF V600E mutation in melanoma patients with monoclonal antibody VE1
Liu, Hui1; Li, Zhongwu1; Wang, Yan1; Feng, Qin1; Si, Lu2; Cui, Chuanliang2; Guo, Jun2; Xue, Weicheng1
关键词Braf V600e Mutation Immunohistochemistry Melanoma
刊名PATHOLOGY INTERNATIONAL
2014-12-01
DOI10.1111/pin.12215
64期:12页:601-606
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Pathology
资助者Beijing Municipal Health Bureau ; Beijing Municipal Health Bureau
研究领域[WOS]Pathology
关键词[WOS]MALIGNANT-MELANOMA ; THYROID-CARCINOMA ; MELANOCYTIC NEVI ; NRAS MUTATIONS ; VEMURAFENIB ; CANCER ; ADENOCARCINOMA ; SURVIVAL ; LESIONS ; GENE
英文摘要

A novel mutation-specific monoclonal antibody VE1 was generated to detect BRAF V600E mutation with immunohistochemistry. This study aims to investigate the sensitivity and specificity of immunohistochemistry compared with conventional Sanger sequencing and to evaluate whether IHC would become the routine screening method of BRAF V600E mutation. A total of 84 cases of melanoma lesion specimens were selected to make the tissue microarray and to perform IHC with VE1 antibody. Simultaneously Sanger sequencing was applied to test and verify. VE1 has a high specificity (100%) and sensitivity (72.2%), and the concordance between the two techniques is excellent (93.8% cases coherent and kappa = 0.801). As a rapid, cost-effective method, IHC may become the routine diagnostic means for the detection of BRAF V600E mutation of malignant melanomas in the near future, and the recommended detection process is initial immunohistochemical staining for positive cases, followed by molecular techniques for negative or ambiguous cases.

语种英语
所属项目编号2010B033 ; 2011-3-088
资助者Beijing Municipal Health Bureau ; Beijing Municipal Health Bureau
WOS记录号WOS:000345842600002
引用统计
被引频次:5[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/53181
专题北京大学临床肿瘤学院_病理科
作者单位1.Peking Univ Canc Hosp & Inst, Dept Pathol, Minist Educ, Key Lab Carcinogenesis & Translat Res, Beijing 100142, Peoples R China
2.Peking Univ Canc Hosp & Inst, Dept Renal Canc & Melanoma, Minist Educ, Key Lab Carcinogenesis & Translat Res, Beijing 100142, Peoples R China
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Liu, Hui,Li, Zhongwu,Wang, Yan,et al. Immunohistochemical detection of the BRAF V600E mutation in melanoma patients with monoclonal antibody VE1[J]. PATHOLOGY INTERNATIONAL,2014,64(12):601-606.
APA Liu, Hui.,Li, Zhongwu.,Wang, Yan.,Feng, Qin.,Si, Lu.,...&Xue, Weicheng.(2014).Immunohistochemical detection of the BRAF V600E mutation in melanoma patients with monoclonal antibody VE1.PATHOLOGY INTERNATIONAL,64(12),601-606.
MLA Liu, Hui,et al."Immunohistochemical detection of the BRAF V600E mutation in melanoma patients with monoclonal antibody VE1".PATHOLOGY INTERNATIONAL 64.12(2014):601-606.
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