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学科主题: 基础医学
题名:
Characterization of OP9 as authentic mesenchymal stem cell line
作者: Gao, Jiao2,3; Yan, Xin-Long2,3; Li, Ren3; Liu, Yi3; He, Wenyan3; Sun, Shengkun4; Zhang, Yu5; Liu, Bing3; Xiong, Jiaxiang1; Mao, Ning2
关键词: OP9 ; mesenchymal stem cells ; mouse ; differentiation ; immunoregulation
刊名: JOURNAL OF GENETICS AND GENOMICS
发表日期: 2010-07-01
DOI: 10.1016/S1673-8527(09)60067-9
卷: 37, 期:7, 页:475-482
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Genetics & Heredity
研究领域[WOS]: Biochemistry & Molecular Biology ; Genetics & Heredity
关键词[WOS]: IN-VITRO DEVELOPMENT ; BONE-MARROW ; ADIPOSE-TISSUE ; STROMAL CELLS ; MOUSE ; ORIGIN ; MICE
英文摘要:

Mesenchymal stem cells (MSCs) are multipotent stem cells capable of differentiating into various cell types, including osteocytes, chondrocytes, adipocytes, myocytes, and tenocytes. However, the difficulty or failure in expanding the mouse MSCs in vitro greatly hampered important research in animal models. The OP9, a stromal cell line from mouse bone marrow, has hematopoietic supportive capacity. Here, we report that the OP9 has the immunophenotype (CD45(-), CD11b(-), FLK-1(-), CD31(-), CD34(-), CD44(+), CD29(+), Sca-1(+), CD86(-), and MHCII(-)) identical to canonical mouse MSCs. The expression of CD140a(+), CD140b(+), alpha-SMA(+) and Calponin(+) suggested the perivascular origin of OP9. Functionally, the OP9 had strong clonogenic ability and could be induced into osteocytes, chondrocytes and adipocytes. The lymphocyte transformation test (LTT) and mixed leukocyte reaction (MLR) showed that the OP9 could suppress T lymphocyte proliferation stimulated by nonspecific mitogens (PHA) or allogeneic lymphocytes (BALB/c T cells). Finally, the migration of OP9 could be efficiently induced by bFGF, IGF-1, IL-3, PDGF-BB, TGF-beta 1 and TGF-beta 3. In conclusion, the OP9 were bona fide MSCs, and such homogenous cell line will be helpful to delineate biological features of MSCs at the stem cell level.

语种: 英语
所属项目编号: 30871410 ; 30600613
项目资助者: National Natural Science Foundation
WOS记录号: WOS:000280313700009
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/53192
Appears in Collections:基础医学院_免疫学系_期刊论文

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作者单位: 1.Inst Basic Med Sci, Dept Cell Biol, Beijing 100850, Peoples R China
2.Third Mil Med Univ, Dept Physiol, Chongqing 400038, Peoples R China
3.Acad Mil Med Sci, Affiliated Hosp, Lab Oncol, Beijing 100071, Peoples R China
4.Peoples Liberat Army Gen Hosp, Dept Urol, Beijing 100853, Peoples R China
5.Peking Univ Hlth Sci Ctr, Dept Immunol, Beijing 100191, Peoples R China

Recommended Citation:
Gao, Jiao,Yan, Xin-Long,Li, Ren,et al. Characterization of OP9 as authentic mesenchymal stem cell line[J]. JOURNAL OF GENETICS AND GENOMICS,2010,37(7):475-482.
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