IR@PKUHSC  > 北京大学基础医学院  > 免疫学系
学科主题基础医学
Characterization of OP9 as authentic mesenchymal stem cell line
Gao, Jiao2,3; Yan, Xin-Long2,3; Li, Ren3; Liu, Yi3; He, Wenyan3; Sun, Shengkun4; Zhang, Yu5; Liu, Bing3; Xiong, Jiaxiang1; Mao, Ning2
关键词OP9 mesenchymal stem cells mouse differentiation immunoregulation
刊名JOURNAL OF GENETICS AND GENOMICS
2010-07-01
DOI10.1016/S1673-8527(09)60067-9
37期:7页:475-482
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Genetics & Heredity
研究领域[WOS]Biochemistry & Molecular Biology ; Genetics & Heredity
关键词[WOS]IN-VITRO DEVELOPMENT ; BONE-MARROW ; ADIPOSE-TISSUE ; STROMAL CELLS ; MOUSE ; ORIGIN ; MICE
英文摘要

Mesenchymal stem cells (MSCs) are multipotent stem cells capable of differentiating into various cell types, including osteocytes, chondrocytes, adipocytes, myocytes, and tenocytes. However, the difficulty or failure in expanding the mouse MSCs in vitro greatly hampered important research in animal models. The OP9, a stromal cell line from mouse bone marrow, has hematopoietic supportive capacity. Here, we report that the OP9 has the immunophenotype (CD45(-), CD11b(-), FLK-1(-), CD31(-), CD34(-), CD44(+), CD29(+), Sca-1(+), CD86(-), and MHCII(-)) identical to canonical mouse MSCs. The expression of CD140a(+), CD140b(+), alpha-SMA(+) and Calponin(+) suggested the perivascular origin of OP9. Functionally, the OP9 had strong clonogenic ability and could be induced into osteocytes, chondrocytes and adipocytes. The lymphocyte transformation test (LTT) and mixed leukocyte reaction (MLR) showed that the OP9 could suppress T lymphocyte proliferation stimulated by nonspecific mitogens (PHA) or allogeneic lymphocytes (BALB/c T cells). Finally, the migration of OP9 could be efficiently induced by bFGF, IGF-1, IL-3, PDGF-BB, TGF-beta 1 and TGF-beta 3. In conclusion, the OP9 were bona fide MSCs, and such homogenous cell line will be helpful to delineate biological features of MSCs at the stem cell level.

语种英语
WOS记录号WOS:000280313700009
项目编号30871410 ; 30600613
资助机构National Natural Science Foundation
引用统计
被引频次:31[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/53192
专题北京大学基础医学院_免疫学系
作者单位1.Inst Basic Med Sci, Dept Cell Biol, Beijing 100850, Peoples R China
2.Third Mil Med Univ, Dept Physiol, Chongqing 400038, Peoples R China
3.Acad Mil Med Sci, Affiliated Hosp, Lab Oncol, Beijing 100071, Peoples R China
4.Peoples Liberat Army Gen Hosp, Dept Urol, Beijing 100853, Peoples R China
5.Peking Univ Hlth Sci Ctr, Dept Immunol, Beijing 100191, Peoples R China
推荐引用方式
GB/T 7714
Gao, Jiao,Yan, Xin-Long,Li, Ren,et al. Characterization of OP9 as authentic mesenchymal stem cell line[J]. JOURNAL OF GENETICS AND GENOMICS,2010,37(7):475-482.
APA Gao, Jiao.,Yan, Xin-Long.,Li, Ren.,Liu, Yi.,He, Wenyan.,...&Mao, Ning.(2010).Characterization of OP9 as authentic mesenchymal stem cell line.JOURNAL OF GENETICS AND GENOMICS,37(7),475-482.
MLA Gao, Jiao,et al."Characterization of OP9 as authentic mesenchymal stem cell line".JOURNAL OF GENETICS AND GENOMICS 37.7(2010):475-482.
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