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Gd-EDDA/HYNIC-RGD as an MR molecular probe imaging integrin alpha nu beta 3 receptor-expressed tumor-MR molecular imaging of angiogenesis
Huo, Tianlong; Du, Xiangke; Zhang, Sen; Liu, Xia; Li, Xubing
关键词Integrin Alphavbeta3/metabolism Arg-gly-asp Rgd Peptides Nude Mice Magnetic Resonance Imaging/methods Hepatocellular Carcinoma/neoplasms
刊名EUROPEAN JOURNAL OF RADIOLOGY
2010-02-01
DOI10.1016/j.ejrad.2008.11.025
73期:2页:420-427
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Radiology, Nuclear Medicine & Medical Imaging
资助者Peking University People&prime ; s Hospital ; Department of Radiology, Peking University People&prime ; s Hospital ; Peking University People&prime ; s Hospital ; Department of Radiology, Peking University People&prime ; s Hospital
研究领域[WOS]Radiology, Nuclear Medicine & Medical Imaging
关键词[WOS]MAGNETIC-RESONANCE ; MOUSE MODEL ; IN-VITRO ; ALPHA(V)BETA(3) ; PEPTIDES ; VASCULATURE ; CELL ; NANOPARTICLE ; DELIVERY ; MICE
英文摘要

Rationale and objective: The aim of this study is to develop a novel MR probe containing arginine-glycine-aspartic acid (RGD) motif for imaging integrin on alpha nu beta 3 receptor-expressed tumor.

Materials and methods: Commercially available HYNIC-RGD conjugated with co-ligand EDDA was labeled with Gd(3+), and the mixture was isolated and purified by solid phase extract (SPE) to get the entire probe Gd-EDDA/HYNIC-RGD. Human hepatocellular carcinoma (HHCC) cell line BEL-7402 was cultured and the cells harvested and suspended in serum-free Dulbecco′s modified Eagle medium (DMEM) were subcutaneously inoculated into athymic nude mice for tumor growth. In vitro cell binding assay to integrin alpha nu beta 3 receptor and cell viability experiments were conducted. The in vivo imaging of the three arms of xenografts were performed by MR scan with a dedicated animal coil at time points of 0, 30, 60, 90 min and 24-h post-intravenous injection (p.i.). Three arms of nude mice then were sacrificed for histological examination to confirm the imaging results.

Results: Gd-EDDA/HYNIC-RGD was successfully isolated by SPE and validity was verified on signal enhancement through in vitro and in vivo experiments. The nude mice model bearing HHCC was well established. There was approx. 30% signal enhancement on T1WI FSE images at 90 min post-intravenous injection of the Gd-EDDA/HYNIC-RGD compared with baseline, and the signal to time curve is straightforward over time in the span of 0-90 min p.i., while the control arms do not show this tendency.

Conclusion: Gd-EDDA/HYNIC-RGD has the potential to serve as an MR probe detecting integrin alpha nu beta 3 receptor-expressed tumor. (C) 2008 Elsevier Ireland Ltd. All rights reserved.

语种英语
资助者Peking University People&prime ; s Hospital ; Department of Radiology, Peking University People&prime ; s Hospital ; Peking University People&prime ; s Hospital ; Department of Radiology, Peking University People&prime ; s Hospital
WOS记录号WOS:000275987600036
引用统计
被引频次:14[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/53195
专题北京大学第二临床医学院_放射科
作者单位Peking Univ, Peoples Hosp, Dept Radiol, Beijing 100044, Peoples R China
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Huo, Tianlong,Du, Xiangke,Zhang, Sen,et al. Gd-EDDA/HYNIC-RGD as an MR molecular probe imaging integrin alpha nu beta 3 receptor-expressed tumor-MR molecular imaging of angiogenesis[J]. EUROPEAN JOURNAL OF RADIOLOGY,2010,73(2):420-427.
APA Huo, Tianlong,Du, Xiangke,Zhang, Sen,Liu, Xia,&Li, Xubing.(2010).Gd-EDDA/HYNIC-RGD as an MR molecular probe imaging integrin alpha nu beta 3 receptor-expressed tumor-MR molecular imaging of angiogenesis.EUROPEAN JOURNAL OF RADIOLOGY,73(2),420-427.
MLA Huo, Tianlong,et al."Gd-EDDA/HYNIC-RGD as an MR molecular probe imaging integrin alpha nu beta 3 receptor-expressed tumor-MR molecular imaging of angiogenesis".EUROPEAN JOURNAL OF RADIOLOGY 73.2(2010):420-427.
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